Comparative Proteomics of Lymphoid Tumour

Authors

  • Kavitha  Assistant Professor, PG and Research Department of Zoology, Quaid-E-Millath Government College for Women (Autonomous) , Chennai, Tamil Nadu, India
  • Dr. C. S. Arunaman  Professor, Department of Clinics and Jurisprudence Madras and Veterinary and Animal Science University Chennai, Tamil Nadu, India

Keywords:

BCL Protein, NCBI, SOPMA, PROTPARAM

Abstract

Comparative analysis of lymphoid tumor protein was conducted in seven protein sequences in the present investigation. The protein sequences were retrieved from NCBI data base for BCL-2, BCL-6, BCL-10, BCL-11. BCL-W, BCL-XL and CD-20. Protein sequence was analysed using Bioinformatics tools such as PROTPARAM for identification of physical and chemical parameters, SOPMA for secondary structure prediction, Clustal W for Multiple sequence analysis, MEGA for constructing Phylogenetic tree were used. Molecular weight, Isoelectric point, instability index, number of amino acids of the seven protein sequences were found and analyzed using PROTPARAM. The number of amino acids present in a protein sequence determines the molecular weight of proteins. Hence the protein BCL-6 weighed more than the other proteins (1040860.0 kDa). The instability Index showed that the protein BCL-W is the stable protein which has the value of less than 40 (33.17). All the other proteins are unstable. The secondary structure predication of the protein was analyzed for alpha helix, Beta sheet, coil region and sequence length to analyze seven protein sequences. An interesting finding of multiple sequence alignment in that the amino acid proline was present in all the proteins of lymphoid tumor and in the species taken for the study. From this it could be inferred that the amino acid proline might be responsible in causing lymphoid tumor. More research needs to be done to target proline amino acid to find out an effective and efficient drug candidates be cure lymphoid tumor. Phylogenetic analysis was carried out and the evolutionary relationship was analyzed. The Bootstrap value was used to predict the reliability of the phylogenetic tree constructed. The phylogenetic tree of the CD-20 showed that the species, Mouse and Monkey are closely related. Cattle and Rat are closely related. Horse and Dog are closely related. This node is somewhat closely related with human. The results of this research work could be utilized for further detailed investigations on the seven proteins involved in lymphoid tumor to formulate measures for the betterment of mankind.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-08-31

Issue

Volume 3, Issue 5, July-August-2017

Section

Research Articles

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How to Cite

[1]
Kavitha, Dr. C. S. Arunaman, " Comparative Proteomics of Lymphoid Tumour, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 5, pp.528-533, July-August-2017.