Comparative Proteomics of Lymphoid Tumour

Authors(2) :-Kavitha, Dr. C. S. Arunaman

Comparative analysis of lymphoid tumor protein was conducted in seven protein sequences in the present investigation. The protein sequences were retrieved from NCBI data base for BCL-2, BCL-6, BCL-10, BCL-11. BCL-W, BCL-XL and CD-20. Protein sequence was analysed using Bioinformatics tools such as PROTPARAM for identification of physical and chemical parameters, SOPMA for secondary structure prediction, Clustal W for Multiple sequence analysis, MEGA for constructing Phylogenetic tree were used. Molecular weight, Isoelectric point, instability index, number of amino acids of the seven protein sequences were found and analyzed using PROTPARAM. The number of amino acids present in a protein sequence determines the molecular weight of proteins. Hence the protein BCL-6 weighed more than the other proteins (1040860.0 kDa). The instability Index showed that the protein BCL-W is the stable protein which has the value of less than 40 (33.17). All the other proteins are unstable. The secondary structure predication of the protein was analyzed for alpha helix, Beta sheet, coil region and sequence length to analyze seven protein sequences. An interesting finding of multiple sequence alignment in that the amino acid proline was present in all the proteins of lymphoid tumor and in the species taken for the study. From this it could be inferred that the amino acid proline might be responsible in causing lymphoid tumor. More research needs to be done to target proline amino acid to find out an effective and efficient drug candidates be cure lymphoid tumor. Phylogenetic analysis was carried out and the evolutionary relationship was analyzed. The Bootstrap value was used to predict the reliability of the phylogenetic tree constructed. The phylogenetic tree of the CD-20 showed that the species, Mouse and Monkey are closely related. Cattle and Rat are closely related. Horse and Dog are closely related. This node is somewhat closely related with human. The results of this research work could be utilized for further detailed investigations on the seven proteins involved in lymphoid tumor to formulate measures for the betterment of mankind.

Authors and Affiliations

Kavitha
Assistant Professor, PG and Research Department of Zoology, Quaid-E-Millath Government College for Women (Autonomous) , Chennai, Tamil Nadu, India
Dr. C. S. Arunaman
Professor, Department of Clinics and Jurisprudence Madras and Veterinary and Animal Science University Chennai, Tamil Nadu, India

BCL Protein, NCBI, SOPMA, PROTPARAM

  1. Roszkowski K.  2014. oxidative DNA damage - the possible use of Biomarkers as additional prognostic factors in oncology. Front biosci.  (Landmark ED.) 19, 808-817,
  2. Gorrini C., Harris I.S., and Mak T.W. 2013. Modulation of oxidative stress as an anticancer strategy. Nat Rev Drug Discov. 12 (12): 931-947.
  3. Jemal, A., Bray, F., Canter, M.M., Ferlay, J., Ward, E. and Forman, D. 2011. Global cancer statistics. CA cancer J clin. 61(2):134.
  4. Benaglia, M. 2009. Recoverable and Recyclable Catalysts, John Wiley and Sons, Chichester.
  5. Cushing, B.L., Kolesnichenko, V.L., Connor, C.J.O. 2004. Chem. Rev., 104, 3893.
  6. Astruc, D. 2008. Nanoparticles and Catalysis;, Ed.; Wiley-VCH: Weinheim.
  7. Vardiman, J.W., Thiele, J., Arber, D.A., Brunning, R.D., Borowitz, M.J., Porwit, A., Harris, N.L., Le Beau, M.M., Hellstrom-Lindberg, E., Tefferi, A. and Bloomfield, C.D. 2008. "The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes.". Blood. 114 (5): 937-51.
  8. Adams, J.M., Cory, S. 2007. The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene. 26(9):1324-1337.
  9. His, E.D. and Yegappan, S. 2001. Lymphoma immunophenotyping: a new era in paraffin-section immunohistochemistry. Adv Anat Pathol. 8:218-39.
  10. Bilalovic, N., Blystad, A.K. and Golouh, R. 2004. Expression of Bcl-6 and CD10 protein is associated with longer overall survival and time to treatment failure in follicular lymphoma. Am J Clin Pathol. 121:34-42.
  11. Meziane, M., Hesse, S., Chetaille, B., Bien -aimee, A., Grob, J.J., Richard, M.A. 2009. Cutaneous Lrge B-cell Lymphoid occurring on a leg burn. Ann Dermatol Venerol., 136(11): 791-794
  12. Weimin, C.I, Jose, M., and Ari Melnick. 2009. B- cell lymphoid 6 and the molecular pathoproteins of diffuse large B- CELL lymphoid. Curr opin Hematol., 15(4): 381-390.
  13. Thome, M. and Weil, R. 2007. Post translational modifications regulate distnict functions of CARMA1 and BCL10. Trends immunol., 28(6) :281-288.
  14. Jost, P., Peschel, C. and Ruland, J. 2006. The Bc110/Malt1 signaling pathway as a drug target in lymphoid. Curr Drug Targets., 7 (10): 1335-40
  15. Satterwhite, E.,  Sonoki, T., Willis, T.G., Harder, L., Nowak, R., Arriola, E.L., Liu, H., Price, H.P., Gesk, S., Steinemnn, D., Schlegelberger, B., Oscier, D.G., Siebert, R., Tucker, P.W. and Dyer, M.J. 2011. The BCL11 protein family: involvement of BCL11A in lymphoid malignancies. Blood., 98 (12):3413-3420.
  16. Uittenbogaaard, M., Baxter, K.K. and Chiaramello, A. 2009. Cloning and characterization of the 5UTR of the rat anti-apoptotic  BCL- w protein. Biochem Biophys Res Commun., 389 (4): 657-662.
  17. Terrano, D.T., Upreti, M. and Chambers, T.C. 2009. CDK1- mediate BCL-XL/BCL-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis. Mol Cell. Biol.
  18. Maeda, A., Iwai, K. and Ishibashi, T. 2009. Sucesssful treatment of T- Cell prolymphocytic leukemia (T-PLL) with fludarabine monophosphate. Rinsho Ketsueki. 50 (8): 658-662.

Publication Details

Published in : Volume 3 | Issue 5 | July-August 2017
Date of Publication : 2017-08-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 528-533
Manuscript Number : IJSRSET11734125
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Kavitha, Dr. C. S. Arunaman, " Comparative Proteomics of Lymphoid Tumour, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 5, pp.528-533, July-August-2017.
Journal URL : http://ijsrset.com/IJSRSET11734125

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