Screening of Cultivable Protease-Producing Bacteria in Marshy Sediments of Mangrove Forests

Authors(2) :-Sarika Chhabria Talreja, Chandra B. Maurya

Mangroves, the coastal wetland forests are the natural inhabitants of variety of microbes especially halophilic bacteria. These halophilic bacteria are prevalent in ocean sediments and play crucial roles in degradation of sedimentary nitrogenous organic materials. The halophilic microorganisms are loaded with potential hydrolytic enzymes to be used in industrial and/or in many biotechnological processes. The bacterial diversity of mangroves in India and variety of their proteases inhabitants are largely unknown. This ambiguity is ascribed to enclosed ecosystem of mangrove region. This study is an attempt to unearth the expediency of mangrove forest for their variety of proteases. For this, bacterial samples were collected from Marshy Sediments of Mangrove Forests, Goa, India and were screened for proteases. Twenty isolates exhibited the proteolytic activity on Casein agar and Gelatin agar plates containing 3% (w/v) NaCl. All the isolates were identified by morphological and molecular characteristics. 16S rRNA sequence analysis showed their homology to Bacillus sp. Qualitative and quantitative enzyme essays revealed potential proteases from B2 and B3 bacterial strains. This study provided novel insights on the community structure of cultivable protease-producing bacteria in the marshy areas of Mangrove forests of Goa.

Authors and Affiliations

Sarika Chhabria Talreja
Assistant Professor, Smt. C.H.M. College, Ulhasnagar, Maharashtra, India
Chandra B. Maurya
Associate Professor, H.O.D., Department of Chemistry, G. N. Khalsa College, Matunga, Mumbai, Maharashtra, India

Proteases, Protease-Producing Bacteria, Marshy Land, Mangroves, 16S Rrna Sequencing.

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Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 61-73
Manuscript Number : IJSRSET117363
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Sarika Chhabria Talreja, Chandra B. Maurya, " Screening of Cultivable Protease-Producing Bacteria in Marshy Sediments of Mangrove Forests, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.61-73, November-December-2017.
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