IJSRSET calls volunteers interested to contribute towards the scientific development in the field of Science, Engineering and Technology

Home > IJSRSET1173852                                                     


Molecular Detection of Torque Teno Virus (TTV) in Plasma of People in Contact with Domestic Village Chickens using Polymerase Chain Reaction (PCR)

Authors(4):

Ahmed Y Mohamed, Abdel Rahim M. El Hussein, Isam M Elkhidir, Khalid A Enan
  • Abstract
  • Authors
  • Keywords
  • References
  • Details
Background : Torque teno virus (TTV) was first detected in 1997 in Japan in a patient with post-transfusion hepatitis. The virus was also detected in the liver and blood of people with hepatic pathologies of unknown etiology. In addition to man,TTV was also found to infect domestic and wild animals including cattle, chickens, pigs, cats, and dogs, etc.
Objective : This study was designed to detect TTV DNA among people who have contact with domestic chickens in Khartoum State during the period December 2016toJune 2017.
Method : A total 100 blood samples were collected,out of these 60 blood samples were collected from people who had contact with domestic village chickens and 40 blood samples were collected from people who had no contact and did not eat chickens. The samples were tested for TTV DNA using nested Polymerase Chain Reaction (nested- PCR) .
Result : TTV DNA was detected in 3(5 %) out of 60 blood samples from people who have contact with domestic chickens and all the samples were negative in the group of people who had no contact and  did not eat chickens.
Conclusion : We observed low TTV prevalence and no association between contact with domestic chickens and TTV infection prevalence was  detected.

Ahmed Y Mohamed, Abdel Rahim M. El Hussein, Isam M Elkhidir, Khalid A Enan

West Nile Viruses (WNV), IgM .ELISA and Real-Time PCR.

  1. Nishizawa T, Okamoto H, Konishi K, Yoshizawa H, MiyakawaY, Mayumi M: A novel DNA virus (TTV) associated with elevated transaminase levels in post tranfusion hepatitis of unknown etiology. Biochem. Biophys Res Commun1997, 241(1):92-97.
  2. Okamoto H, Nishizawa T, Kato N, Ukita M, Ikeda H, Iizuka H: Molecular cloning and characterization of a novel DNA virus (TTV) associated with post transfusion hepatitis of unknown etiology. Hepatol Res1998,10(1):1-16.
  3. Watanabe MA, Miranda HC, Oliveira KB, Oliveira CE, Tristão FS, Duarte LM: Prevalence of Torque teno virus in healthy donors of Paraná State, southern Brazil. Rev Bras Hematol Hemoter2005,41(4):223-8.
  4. Nasser TF, Brajão de Oliveira K, Reiche EM, Amarante MK,Pelegrinelli Fungaro MH, Watanabe MA. Detection of TT virus in HIV-1 exposed but uninfected individuals and in HIV-1infected patients and its influence on CD4+ lymphocytes and viral load: Microb Pathog2009,47(1):33-7.
  5. Costa MR, Costa IP, Devalle S, Castro ARCM, Freitas SZ: Prevalence and genetic diversity of torque teno virus in patients with systemic lupus erythematosus in a reference service in Mato Grosso do Sul. Rev Bras Reumatol 2012,52(1):49-54.
  6. Maggi F, Pifferi M, Fornai C, Andreoli E, Tempestini E,Vatteroni M: TT virus in the nasal secretion of children with acute respiratory diseases: relations to viremia and disease severity. J Virol 2003,77(4):2418-25.
  7. Girard C, Ottomani L, Ducos J, Dereure O, Carles MJ, GuillotB: High prevalence of Torque Teno (TT) virus in classical Kaposi’s sarcoma. Acta Derm Venereol2007,87(1):14-7.
  8. Bendinelli M, Pistello M, Maggi F, Fornai C: Molecular properties,biology, and clinical implications of TT Virus, a recently Identified widespread infectious agent of humans. Clin Microbiol Rev 2001, 14:98-113.
  9. Nishizawa T, Okamoto H, Tsuda F, Aikawa T, Sugai Y, Konishi K, AkahaneY, Ukita M, Tanaka T, Miyakawa Y, Mayumi M: QuasispeciesofTT Virus (TTV) with sequence divergence in hypervariable regions of the capsid protein in chronic TTV infection. J Virol 1999, 73:9604-9608.
  10. Gallian P, Berland Y, Olmer M, Raccah D, de Micco P, Biagini P, SimonS, Bouchouareb D, Mourey C, Roubicek C, Touinssi M, CantaloubeJF,Dussol B, de Lamballerie X: TT virus infection in French hemodialysis patients: Study of prevalence and risk factors.J Clin Microbiol1999, 37:2538-2542.
  11. Hsieh S-Y, Wu Y-H, Ho Y-P, Tsao K-C, Yeh C-T, Liaw Y-F: High Prevalence of TT Virus infection in healthy children and adults and in Patients with Liver Disease in Taiwan. J Clin Microbiol 1999, 37:1829-1831.
  12. Hafez MM, Shaarawy SM, Hassan AA, Salim RF, El Salam FMA, Ali AE: Prevalence of transfusion transmitted virus (TTV) genotypes among HCC patients in Qalupbia governorate. Virol J 2007, 4:135.
  13. Abe K, Inami T, Ishikawa K, Nakamura S, Goto S: TT Virus infection in Non-human primates and characterization of the viral genome: identification of Simian TT virus isolates. J Virol 2000, 74:1549-1553.
  14. Maggi F, Andreoli E, Lanini L, Fornai C, Vatterloni M, Pistello M, Presciuttini S, Bendinelli M: Relationships between Total Plasma Load of Torquetenovirus (TTV) and TTV Genogroups Carried. J ClinMicrobiol 2005, 43:4807-4810.
  15. Krekulova L, Rehak V, Killoran P, Madrigal N, Riley LW: Genotypicdistribution of TT virus (TTV) in a Czech population: evidence for sexual transmission of the virus. J ClinVirol 2001,23:31-41.
  16. MacDonald DM, Scott GR, Clutterbruck D, Simmonds P: Infrequent detection of TT virus infection in intravenous drug users, prostitutes and homosexual men. J Infect Dis 1999,179:686-689.
  17. Gerner P, Oettinger R, Gerner W, Falbrede J, Wirth S: Mother-to infant transmission of TT virus: prevalence, extent and mechanism of vertical transmission. Pediatr Infect Dis J 2000,19:1074-1077.
  18. Iso K, Suzuki Y, Takayama M: Mother-to-infant transmission of TT virus in Japan. Int J GynaecolObstet 2001, 75:11-19.
  19. Griffin JS, Plummer JD, Long SC: Torque teno virus: an improved indicator for viral pathogens in drinking waters. Virol J 2008,5:112.
  20. Haramoto E, Katayama H, Oguma K, Ohgaki S: Application of cation-coated filter method to detection of noroviruses, enteroviruses, adenoviruses, and torque teno viruses in the Tamagawa river in Japan. Appl Environ Microbiol 2005,71:2403-2411.
  21. Irshad M, Joshi YK, Sharma Y, Dhar I: Transfusion transmitted virus: A review on its molecular characteristics and role in medicine. World J Gastroenterol 2006, 12:5122-5134.
  22. Shibayama T, Masuda G, Ajisawa A, Takahashi M, Nishizawa T, TsudaF, Okamoto H: Inverse relationshio between the titre of TTvirus DNA and the CD4 cell count in patients infected with HIV. AIDS 2001, 15:563-570.
  23. McLaughlin-Dubin ME, Munger K: Viruses associated with human cancer:Biochim Biophys Acta 2008, 1782:127-150.
  24. Blasek A, Sillo P, Ishii N, Gergely P, Poor G, Preisz K, Hashimoto T,Medvecz M, Karpati S: Searching for foreign antigens as possible triggering factors of autoimmunity: Torque Teno virus DNA prevalence is elevated in sera of patients with bullous pemphigoid.Exp Dermatol 2008, 17:446-454.
  25. Mushahwar IK. Recently discovered blood-borne viruses: are they hepatitis viruses or merely endosymbionts? J Med Virol 2000: 62 : 399-404.
  26. Bouzari M, ShaykhBaygloo N: Detection of torque teno virus (TTV) in domestic village chickens in Iran. Winter 2013,4(1):55-8
  27. CATROXO M.H.B, NISHIYA A, SABINO E., TEIXEIRA P.S, PETRELLA S, MILANELO L,VIEIRA J.C.F, DIAZ R.S: Research of Torque teno virus (TTV) in serum and total blood of non-human primates and in chicken plasma (Gallus gallus domesticus)by the PCR-N22 region. Int. J. Morphol 2008, 26(2):377-384.
  28. Campo N, Brizzolara R, Sinelli N, Torre F, Russo R, Deferrari G and Picciotto A:TT-virus infection in hemodialysis patients. Nephrol Dial Transplant2000,15: 1823-6.
  29. Gallian P, Berland Y, Olmer M, Raccah D, De Micco P,:TT-virus infection in French hemodialysis patients: study of prevalence and risk factors. J Clin Microbiol 1999, 37: 2538-42.
  30. Abe K, Inami T, Asano K : TT virus infection is wide spread in the general populations from different geographic regions. J ClinMicrobiol. 1999,37: 2703 - 2709.
  31. Pistello M, Morrica A, Maggi F:TTV levels in the plasma of infected individuals with different hepatic and extra-hepatic pathology. J Med Virol. 2001, 63:189-195.
  32. Hind Azhari, Isam M. Elkhidir, Abdel Rahim M. El Hussein, Ahamed M Alamin, Mohamed El-Fatih, Khalid A. Enan:Molecular Detection of Torque Teno Virus (TTV) Infection among Positive HBV Patients in Khartoum State, Sudan.EUROPEAN ACADEMIC RESEARCH 2015, 2286-4822.
  33. Alamin AM, Enan KA, Elkhidir IM:Molecular detection of Torque teno virus in healthy blood donors in Khartoum-state, Sudan. MSc.Thesis 2009,Alneelain University.

Publication Details

Published in : Volume 3 | Issue 8 | November-December - 2017
Date of Publication Print ISSN Online ISSN
2017-12-31 2395-1990 2394-4099
Page(s) Manuscript Number   Publisher
250-254 IJSRSET1173852   Technoscience Academy

Cite This Article

Ahmed Y Mohamed, Abdel Rahim M. El Hussein, Isam M Elkhidir, Khalid A Enan, "Molecular Detection of Torque Teno Virus (TTV) in Plasma of People in Contact with Domestic Village Chickens using Polymerase Chain Reaction (PCR)", International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.250-254, November-December-2017.
URL : http://ijsrset.com/IJSRSET1173852.php