Serological and Molecular Detection of Chikungunya Virus among Arthritis Patient in Khartoum State, Sudan

Authors(5) :-Alafra A Awad, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan

Background: Chikungunya virus is an arthropod-borne virus belonging to the Togaviridae family and the genus Alphavirus. Its symptoms imitate rheumatoid arthritis (RA) and should be suspected in patients with RA-like features in endemic areas.
Method: This is a descriptive, cross sectional study performed to detect IgM antibody and genome of CHIKV in patients with arthritis symptoms who attended the Military Hospital in Khartoum, Sudan during June 2017 to December 2017. CHIKV IgM antibodies and CHIKV RNA were estimated in 90 patients using ELISA and Reverse transcriptase Real time PCR respectively.
Results: out of 90 patients with arthritis symptoms; Rheumatoid factor and anti Cyclic Citrullinated Peptide were negative, 66.3% were complaining of fever 52.6 % have high Erythrocyte sedimentation rate (ESR), all patients were complaining of arthralgia, 22.9% in Fingers, 24.3% in Wrists 16.7% in Knees, 26% in Ankles, and 10.1% in Elbows, three samples were positive for CHIKV IgM (3.3%)., and six were positive for CHIKV RNA (6.7%).

Conclusions: CHIKV IgM antibodies and CHIKV RNA were detected among arthritis patients in Khartoum, Sudan. Further investigations are required to delineate the distribution and the importance of CHIKV in the etiology of arthritis and other disease syndromes in Sudan.

Authors and Affiliations

Alafra A Awad
Department of Medical microbiology, Faculty of Medical Laboratory Science, El Nellin University, Khartoum, Sudan
Abdel Rahim M El Hussein
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
Mohamed O. Mustafa
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
Isam M Elkhidir
Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
Khalid A Enan
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan

Chikungunya virus, ELISA , anti ccp, Reverse transcriptase Real time PCR .

  1. Robinson MC. An epidemic of virus disease in Southern Province,Tanganyika Territory in 1952–53—1: clinical features. Trans R Soc Trop Med Hyg. 1955;49:28.
  2. Tsetsarkin KA, Vanlandingham DL, McGee CE, Higgs S: A single mutation in chikungunya virus affects vector specificity and epidemic potential. PLoSPathog. 2007, 3:e201.
  3. Thiberville SD, Moyen N, Dupuis-Maguiraga L, et al. Chikungunya fever: epidemiology, clinical syndrome, pathogenesis and therapy. Antivir Res. 2013;99:345–70.
  4. Schilte C, Staikowsky F, Couderc T, et al. Chikungunya virusassociated long-term arthralgia: a 36 month prospective longitudinal study. PLoS Negl Trop Dis. 2013;7, e2137. doi:10.1371/ journal.pntd.0002137.
  5. Mathew AJ, Goyal V, George E, Thekkemuriyil DV, Jayakumar B,Chopra A. Rheumatic-musculoskeletal pain and disorders in anaïve group of individuals 15 months following a Chikungunya viral epidemic in south India: a population based observational study. Int J Clin Pract. 2011;65:1306–12.
  6. Borgherini G, Poubeau P, Staikowsky F, et al. Outbreak of chikungunyaon Réunion Island: early clinical and laboratory features in 157 adult patients.ClinInfectDis.Jun1 2007;44(11):1401-1407.
  7. Taubitz W, Cramer JP, Kapaun A, et al. Chikungunya fever in travelers: clinical presentation and course. Clin Infect Dis. Jul 1 2007;45(1):e1-4.
  8. Jupp PG, McIntosh BM. Chikungunya virus disease. In: Monath TP, ed. The Arboviruses: Epidemiology and Ecology. Vol II. Boca Raton, FL: CDC Press, Inc.; 1988.
  9. Ryman KD, Klimstra WB. Closing the gap between viral and noninfectious arthritis. Proc Natl Acad Sci U S A. 2014;111:5767–8.
  10. Adam A, Seidahmed OM, Weber C, Schnierle B, Schmidt-Chanasit J, Reiche S, Jassoy C. Low Seroprevalence Indicates Vulnerability of Eastern and Central Sudan to Infection with Chikungunya Virus. Vector-Borne and Zoonotic Diseases. 2016 Apr 1;16(4):290-1.
  11. Mohamed HM Isa, Abbas B Mohammed,Wafa I Elhag : Serofrequency of Chikungunya Virus Among Blood Donors in Singa City Sinnar State, Sudan 2017; International Journal of Innovative Science and Research Technology Vol 2 (12). Dec 2017 . 165-169 .
  12. Sissoko D, Moendandze A, Malvy D, Giry C, Ezzedine K, Solet J-L,Pierre V: Seroprevalence and risk factors of chikungunya virusinfection in Mayotte, Indian Ocean, 2005-2006: a populationbased survey. PLoS ONE 2008, 3:e3066.
  13. Lokireddy S, Vemula S, Vadde R: Connective tissue metabolism in Chikungunya patients. Virol J 2008, 5:31.
  14. Adam A, Seidahmed OM, Weber C, Schnierle B, Schmidt-Chanasit J, Reiche S, Jassoy C. Low Seroprevalence Indicates Vulnerability of Eastern and Central Sudan to Infection with Chikungunya Virus. Vector-Borne and Zoonotic Diseases. 2016 Apr 1;16(4):290-1.
  15. Gould LH, Osman MS, Farnon EC, Griffith KS, Godsey MS, Karch S, Mulenda B, El Kholy A, Grandesso F, de Radiguès X, Brair ME. An outbreak of yellow fever with concurrent chikungunya virus transmission in South Kordofan, Sudan, 2005. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2008 Dec 1;102(12):1247-54.
  16. Ahmed Y Rodwan,Abbas Bm Rahama, Rania S Ahmed, Nazar M Abdalla, Amani A Osman, Mustafa E Yassin: Seroprevalence of Chikungunya virus within arthritis patients in Khartoum, Sudan 2016. European Academic Research Vol. IV, June 2016. 2210- 2220 .
  17. Moyen N,Thiberville S-D,Pastorino B,Nougairede A, Thirion L,Mombouli J-V et al .(2014) Frist Reported Chikungunya Fever Outbreak in the Republic of Congo,2011. PLoS ONE 9 (12):e 115938. Doi:10.1371.

Use the online HTML, CSS, JavaScript tool collection to make websites easily.

Publication Details

Published in : Volume 4 | Issue 9 | July-August 2018
Date of Publication : 2018-07-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 112-116
Manuscript Number : IJSRSET1184459
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Alafra A Awad, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Serological and Molecular Detection of Chikungunya Virus among Arthritis Patient in Khartoum State, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 9, pp.112-116, July-August.2018
URL : http://ijsrset.com/IJSRSET1184459

Follow Us

Contact Us