The aim of this study was to investigate the protective and adverse effects of 10% of raw broccoli on lead acetate induced oxidative stress in rats. Fifty six male rats were divided into eight groups, seven rats in each. Control group injected with distilled water, Br group were fed on 10% broccoli for five weeks ,LE6 group were injected intraperitoneally with 6 mg/kg body weight lead acetate for five weeks ,LE10 group were injected intraperitoneally with 10 mg/kg body weight lead acetate for five weeks ,Br+LE6 group were injected intraperitoneally with 6 mg/kg body weight lead acetate and feed on 10% broccoli concomitantly for five weeks , and Br+LE10 group were injected intraperitoneally with 10 mg/kg body weight lead acetate and feed on 10%broccoli concomitantly for five weeks.While LE6+Br group was injected previously with 6mg/kg of body weight lead acetate for three weeks and then fed on 10% broccoli and injected with lead acetate concomitantly for two weeks and then fed on broccoli alone for the last three weeks. Last group was LE10+Br group was injected previously with 10mg/kg of body weight lead acetate for three weeks and then fed on 10% broccoli and injected with lead acetate concomitantly for two weeks and then fed on broccoli alone for the last three weeks. Our result revealed that the SOD% and total glutathione increased significantly (p<0.05)in group Br and decreased significantly (p<0.05) in group LE6and LE10 in dose-dependent manner compare with group C .while the levels increased significantly in all Broccoli treated groups.Moreover, the results revealed that the broccoli was more effective in the groups that injected with lead acetate and fed on broccoli concomitantly. In another hand, the level of MDA Âµmol/ml increased significantly (p<0.05) in group LE6 and LE10 compare with group C while decreased in all Broccoli treated groups. In conclusion, broccoli at a dose of 10% exhibited a protective effect on lead acetate induced oxidative stress in rats, also it had ability to alleviate and adverse the Pb toxicity by the improvement of the antioxidant system.
Sulaf Mustafa Mohammed , Mousa Jasim Mohammed , Ebtisam Al Aboosi
antioxidant, oxidative stress, lead acetate, broccoli
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Cite This Article
Sulaf Mustafa Mohammed , Mousa Jasim Mohammed , Ebtisam Al Aboosi , "Effects of Broccoli on Oxidative Stress Produced by Lead Acetate in Male Albino Rats Rattus Rattus", International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 4, pp.153-158, July-August-2015.
URL : http://ijsrset.com/IJSRSET151411.php