Studies on Immobilized Spores of Aspergillus Fumigatus

Authors(2) :-Ratnasri P. V, Hemalatha K. P. J

In this present study, the effect of immobilized spores of Aspergillus fumigatus (MTCC NO. 1399) on amylase production was investigated on four different matrices like agar agar, agarose, sodium alginate and k-Carrageenan. Amylase activity was found to be increased by 11.4% with sodium alginate at 72hrs of incubation when compared with the free spores. The 3rd repeated cycle of immobilized spores of A.fumigatus showed (283±34U/ml) amylase activity and increase percent of amylase activity was 45% when compared to initial amylase activity. The amylase production was decreased for further usage.

Authors and Affiliations

Ratnasri P. V
Department of Microbiology, Andhra University, Visakhapatnam-530 003, Andhra Pradesh, India
Hemalatha K. P. J
Department of Microbiology, Andhra University, Visakhapatnam-530 003, Andhra Pradesh, India

Aspergillus Fumigatus, Immobilization of Spores, Sodium Alginate, Repeated Cycles, Amylase Activity.

  1. Margaritis A and Merchant FJA (1984). Advances in ethanol production using immobilized cell systems. Crit. Rev. Biotechnol., 2: 339–393.
  2. Stewart GG and Russel I (1986). One hundred years of yeast research and development in the brewing industry. J. Inst Brew., 92: 537–558.
  3. Shah ND, Chattoo BB, Kothari BRM and Hegde PVM (1993). Starch hydrolysis 45: 104-109.
  4. Hemachander C, Bose N, Puvanakrishnan R (2001) Whole cell immobilization of Ralstonia pickettii for lipase production. Process Biochem., 36: 629–633.
  5. Sun Y and Furusaki S (1990). Continuous production of acetic acid from Acetobacter aceti in a fluidized bed bioreactor. J. Ferment Bioeng., 69: 102-110.
  6. Wang Bao-e and HU Yong-you (2007). Comparison of four supports for adsorption of reactive dyes by immobilized Aspergillus fumigatus beads. Journal of Environmental Sciences. 19: 451–457.
  7. Angelova MB (2000). Comparison of antioxidant enzyme biosynthesis by free and immobilized Aspergillus niger cells. Enz. Microb. Technol., 26, 544-549.
  8. Ellaiah P (2004). Production of lipase by immobilized cells of Aspergillus niger. Process Biochem., 39,525-528.
  9. Nighojkar S (2006). Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel as inducer. Process Biochem .,.41,1136-1140.
  10. Efremenko EN (2006). L(+)-lactic acid production using PVA-cryogel entrapped Rhizopus oryzae fungus cells. J. C hem. Technol. Biotech., 81, 519-522.
  11. Koshcheyenko KA, Turkina MV and Skryabin GK (1983). Immobilization of living microbial cells and their application for steroid transformation. Enzyme and Microbial Technology. 5, 14-21.
  12. MacCabe AP, Orejas M, Tamayo EN, Villanueva A and Ramón D (2002) Improving extracellular production of food-use enzymes from Aspergillus nidulans: Review. J. Biotechnol., 96, 43-54.
  13. Elisashvili V, Penninckx M, Kachlishvili E, Tsiklauri N, Metreveli (2008). Lentinus edodes and Pleurotus species ligno cellulolytic enzymes activity in submerged and solid state fermentation of lignocellulosic wastes of different composition. J. Biores. Technol., 99, 457-462.
  14. Correa MG, Ludeña Y, Ramage G and Villena GK (2012). Recent advances on filamentous fungal biofilms for industrial uses. Appl. Biochem. Biotechnol., 167, 1235 - 1253.
  15. Selwal MK and Selwal KK (2012). High-level tannase production by Penicillium atramentosum KM using agro residues under submerged fermentation. Ann. Microbiol., 62, 139-148.
  16. Ratnasri PV, Lakshmi BKM, Ambica devi K and Hemalatha KPJ (2014a). Isolation,characterization of Aspergillus fumigatus and optimization of cultural conditions for amylase production. International journal of research in engineering and technology. 3(2): 457-463.
  17. Francis Borgio (2011). Immobilization of microbial (wild strains) amylase on Alginate matrix and Coconut fiber for enhanced activity. American Journal of Biochemistry and molecular biology. 1(3): 255-264.
  18. Nilson K, Birnbaum S and Flygare S (1983). A general method for the immobilization of cells with preserved viability. Eur J Appl Microbiol Biotechnol., 17: 319-326.
  19. Vassileva M, Azcon R, Barea J and Vassilev N (1998). Application of an encapsulated filamentous fungus in solubilization of inorganic phosphate. J. Biotechnol., 63, 67–72.
  20. Wada M, Kato J and Chibata I (1979). Immobilization of cells. Eur. J Appl Microbiol Biotechnol., 8: 241-252
  21. Eikmeirer H, Westmeiier and Rehm HJ (1984). Morphological development of Aspergillus niger immobilized in Ca- alginate and K-carrageenan. Appl. Microbiol. Biotechnol., 19: 53-57
  22. Van Elsas JD and Heijnen CE (1990). Methods for the introduction of bacteria into soil: a review. J. Biol Fertil Soils. 10: 127–133.
  23. Khare SK, Jha K and Gandhi AP (2004). Use of agarose- entrapped Aspergillus niger cells for production of citric acid from soy whey. Appl. Microbiol. Biotechnol., 41: 571-573.

Publication Details

Published in : Volume 1 | Issue 4 | July-August 2015
Date of Publication : 2015-08-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 396-400
Manuscript Number : IJSRSET151483
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Ratnasri P. V, Hemalatha K. P. J, " Studies on Immobilized Spores of Aspergillus Fumigatus, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 4, pp.396-400, July-August-2015.
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