Propolis an Antibacterial Agent Against Clinical Isolates of Wound Infection

Authors

  • Dr. Farida Iftikhar  PSO, Honeybee Research Institute, NARC, Islamabad, Pakistan
  • Dr. Rashid Mahmood  PSO, Honeybee Research Institute, NARC, Islamabad, Pakistan

Keywords:

Propolis, Compositions, Antimicrobial, Effect, MIC

Abstract

Propolis is a resinous mixture that is collected by the honeybees from the plants. The physical character of propolis generally has been used by honeybees to protect their hive but it has several beneficial properties for human beings. In the present study four different propolis samples were collected and subjected for extraction using ethanol (95%) as solvent. Their antimicrobial effect was evaluated against different bacterial strains isolated from wound infected patients at a local hospital in Islamabad including S. aures,  E. coli,  P. aeruginosa and K. pneumonia by agar well diffusion technique.Two strains i.e. S. aures (ATCC No. 25923) and E. coli (ATCC No. 25922) were used as control. Minimum inhibitory concentration (MIC) was also determined by agar well diffusion technique.  In addition, the bioactive compounds and functional groups of the extracts were determined by paper chromatography and Spray methods. The results obtained indicated that ethanol extracts of the propolis showed antimicrobial Effect. Our finding concerning to the chemical analysis of the propolis exhibited the presence of flavonoid, tannin, steroid, alcohol and alkaloid in extracts. Overall, propolis has antimicrobial effect with different spectrum and therefore, it might consider a potent candidate for treatment of several clinical scenarios.

References

  1. Anskota A.,Y .Tezka, S. Kdota. Recent progress in pharmacological research of propolis. Phytotherres . 2011. pp.561-571.
  2. Atec U, S. Kadriye, O. Bekir S .Chemical composition and antimicrobial activities of four Anatolian propolis samples. Microbial research pp. 186-195.
  3. Ayhan T.,S. Ayla, O. T. Asli,S. Kadriye, S. Bekir .Antibacterial activity of bee propolis sample from different geographical regions of turkey against two food borne pathogens, salmonella Enteritidis and listeria monocytogenes Turk J Biol. 2011. p.35305-511.
  4. Bankova V. S., S. L. De Castro, and M. C. Marcucci, “Propolis: recent advances in chemistry and plant origin,” Apidologie, vol. 31, no. 1, pp. 3–15, 2000.
  5. Boyanova L, Kolarov R, Gergova G, Mitov I. In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. Anaerobe 2006;12(4):173–7.
  6. Burdock G. A., “Review of the biological properties and toxicity of bee propolis (propolis),” Food and Chemical Toxicology, vol. 36, no. 4, pp. 347–363, 1998.
  7. Castaldo S., F. Capasso.Propolis, an old remedy used in modern medicine Fitoterapia 2002. pp. 1–6.
  8. Castro S. L. De, “Propolis: biological and pharmacological activities.Therapeutic uses of this bee-product,” Annual Reviewof Biomedical Sciences, vol. 3, pp. 49–83, 2001.
  9. Forcing S., J. Bankova. Propolis:is there a potential for the development of new drug. J Ethnopharmacaol . 2011. pp. 253-240.
  10. Ghisalberti E. Propolis : a review. World 1979. pp. 59-84.
  11. Hausen B. M., E.Wollenweber,H. Senff, and B. Post, “Propolis allergy. (II). The sensitizing properties of 1,1-dimethylallyl caffeic acid ester,” ContactDermatitis, vol. 17,no. 3, pp. 171–177, 1987.
  12. Helfenberg K. D., “The analysis of beeswax and propolis,” ChemikerZeitungm, vol. 31, pp. 987–998, 1908.
  13. Jork  H., W. Funk, H. Wimmer, N. Wiley. TLC Reagents & Detection methods-physical & chemical detection methods: Activation Reactions, Sequences, Reagents II, Vol 1b, illuminatiy 1994
  14. Journal of Venomous Animals and Toxins Including Tropical Diseases 2008. pp. 357–365.
  15. Mantovani R., V. Rall , J. Batalha , A. Fernandes, Anticoagulase- negative Staphylococcus activity of ethanolic extracts of propolis from two Brazilian regions and synergism with antimicrobial drugs by the E-test method.
  16. Marcucci M., “Propolis: chemical composition, biological properties and therapeutic activity,” Apidologie, vol. 26, no. 2, pp. 83–99, 1995.
  17. Markham K., K. Mitchell, A. Wilkins, J. Daldy , Y. lu. HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry 1996. pp. 205-211.
  18. Milena P., D. Rosa, T. Hassan, T. Iva, N. Hristo, B.Vassya. Omani propolis: Chemical profiling, antibacterial activity and new propolis plant sources. Chemistry Journal. 2013. p.158
  19. Monti M., E. Berti, G. Carminati, andM. Cusini, “Occupational and cosmetic dermatitis frompropolis,” ContactDermatitis, vol. 9, no. 2, p. 163, 1983.
  20. Nallahalli S.,B. Musaiah, R. Hemagirigowda. Antimicrobial activity of propolis of trigona Sp. and Apismellifera of Karnataka, India, Prime Journal of Microbiology Research. 2008. pp. 80-85.
  21. Park Y. K., S. M. Alencar, and C. L. Aguiar, “Botanical origin and chemical composition of Brazilian propolis,” Journal ofAgricultural and Food Chemistry, vol. 50, no. 9, pp. 2502–2506, 2002.
  22. Park Y., J. Paredes-Guzman, C. Aguiar, S. Alencar, F. Fujiwara. Chemical constituents in Baccharisdracunculifoliaas the main botanical origin of Southeastern Brazilian propolis Journal of Agricultural and Food Chemistry. 2004. pp. 1100–1103.
  23. Park Y., J. Paredes-Guzman, C. Aguiar, S. Alencar, F. Fujiwara. Chemical constituents in Baccharisdracunculifoliaas the main botanical origin of Southeastern Brazilian propolis Journal of Agricultural and Food Chemistry. 2004. pp. 1100–1103.
  24. Pietta P. G., C. Gardana, and A. M. Pietta, “Analytical methods for quality control of propolis,” Fitoterapia, vol. 73, no. 1, pp. S7– S20, 2002.
  25. Silvia H, J. Marcos, W. Evandro, Y. Izabel, C. Dioneia. antimicrobial activity of flavonoids and sreroids isolated from two Chromolaena species. Brazilian journal of pharmaceutical scinces. 2013. P. 4. out./dez.
  26. Trease G.,W.Evans.Pharmacognosy(15thEd),Saunders company, London.20 Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M,UskudarGuclu A, et al. In vitro antimicrobial activity of propolissamples from different geographical origins against certain oralpathogens. Anaerobe 2007;13(3-4):140–5.
  27. Vassya S., D. Solangel , R. Cast, C. Mari. Review article. Propolis: recent advances in chemistry and plant origin. Apidologie. 2000. pp. 313-15.
  28. Vijay D.WaghPropolis: A Wonder Bees Product and Its Pharmacological Potentials Advances in Pharmacological Sciences Volume 2013, Article ID 308249, 11 pages
  29. Wollenweber E., B. M. Hausen, and W. Greenaway, “Phenolic constituents and sensitizing properties of propolis, poplar balsam and balsam of Peru,” Bulletin de Liaison—GroupePolyphenols, vol. 15, pp. 112–120, 1990.
  30. Yaghoubi  S., G. Ghorbani, R. SoleimanianZad,sattari . antimicrobial activity of Iranian propolis and its chemical composition. DARU. 2007. pp. 45-48.

International Journal of Scientific Research in Science, Engineering and Technology

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Published

2015-12-25

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Volume 1, Issue 6, November-December-2015

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Research Articles

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How to Cite

[1]
Dr. Farida Iftikhar, Dr. Rashid Mahmood, " Propolis an Antibacterial Agent Against Clinical Isolates of Wound Infection, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 6, pp.47-52, November-December-2015.