In Vitro Apical Culture of Musa Paradisiaca cv. Gaja Bantal

Authors(2) :-Bandita Deo, Bikram Pradhan

Explants with apical domes have been reported to have higher rate of survival. In vitro propagation of banana (Musa spp.) from the apical explants isolated from the sword suckers is described here. Explants with apical domes were successfully cultured on Murashige and Skoog’s medium containing 6-Benzyl Amino Purine , Kinetin , Indole -3-acetic acid , Naphathalene acetic acid and Adenine Sulphate. Multiple shoot initial could be produced from shoot tip explants in presence or absence of apical domes but the survival rates of explants were high when the apical domes were retained. From the in vitro apical culture of banana it was found out that 3 mg/lt BAP + 3 mg/lt KN + 1 mg/lt IAA + 150 mg/lt ADS induction medium and 3 mg/lt BAP + 1 mg/lt IAA + 0.25 mg/lt NAA + 75 mg/lt ADS multiplication medium shows remarkable results during different phases of in vitro culture.

Authors and Affiliations

Bandita Deo
Plant Physiology and Biochemistry division, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, India
Bikram Pradhan
Plant Physiology and Biochemistry division, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, India

Explants with apical domes have been reported to have higher rate of survival. In vitro propagation of banana (Musa spp.) from the apical explants isolated from the sword suckers is described here. Explants with apical domes were successfully cultured on Murashige and Skoog’s medium containing 6-Benzyl Amino Purine , Kinetin , Indole -3-acetic acid , Naphathalene acetic acid and Adenine Sulphate. Multiple shoot initial could be produced from shoot tip explants in presence or absence of apical domes but the survival rates of explants were high when the apical domes were retained. From the in vitro apical culture of banana it was found out that 3 mg/lt BAP + 3 mg/lt KN + 1 mg/lt IAA + 150 mg/lt ADS induction medium and 3 mg/lt BAP + 1 mg/lt IAA + 0.25 mg/lt NAA + 75 mg/lt ADS multiplication medium shows remarkable results during different phases of in vitro culture.

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Publication Details

Published in : Volume 1 | Issue 5 | September-October 2015
Date of Publication : 2015-10-16
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 284-289
Manuscript Number : IJSRSET151561
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Bandita Deo, Bikram Pradhan, " In Vitro Apical Culture of Musa Paradisiaca cv. Gaja Bantal, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 5, pp.284-289, September-October-2015.
Journal URL : http://ijsrset.com/IJSRSET151561

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