In Vitro Apical Culture of Musa Paradisiaca cv. Gaja Bantal

Authors

  • Bandita Deo  Plant Physiology and Biochemistry division, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, India
  • Bikram Pradhan  Plant Physiology and Biochemistry division, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, India

Keywords:

Explants with apical domes have been reported to have higher rate of survival. In vitro propagation of banana (Musa spp.) from the apical explants isolated from the sword suckers is described here. Explants with apical domes were successfully cultured on Murashige and Skoog’s medium containing 6-Benzyl Amino Purine , Kinetin , Indole -3-acetic acid , Naphathalene acetic acid and Adenine Sulphate. Multiple shoot initial could be produced from shoot tip explants in presence or absence of apical domes but the survival rates of explants were high when the apical domes were retained. From the in vitro apical culture of banana it was found out that 3 mg/lt BAP + 3 mg/lt KN + 1 mg/lt IAA + 150 mg/lt ADS induction medium and 3 mg/lt BAP + 1 mg/lt IAA + 0.25 mg/lt NAA + 75 mg/lt ADS multiplication medium shows remarkable results during different phases of in vitro culture.

Abstract

Explants with apical domes have been reported to have higher rate of survival. In vitro propagation of banana (Musa spp.) from the apical explants isolated from the sword suckers is described here. Explants with apical domes were successfully cultured on Murashige and Skoog’s medium containing 6-Benzyl Amino Purine , Kinetin , Indole -3-acetic acid , Naphathalene acetic acid and Adenine Sulphate. Multiple shoot initial could be produced from shoot tip explants in presence or absence of apical domes but the survival rates of explants were high when the apical domes were retained. From the in vitro apical culture of banana it was found out that 3 mg/lt BAP + 3 mg/lt KN + 1 mg/lt IAA + 150 mg/lt ADS induction medium and 3 mg/lt BAP + 1 mg/lt IAA + 0.25 mg/lt NAA + 75 mg/lt ADS multiplication medium shows remarkable results during different phases of in vitro culture.

References

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2015-10-16

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Volume 1, Issue 5, September-October-2015

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Research Articles

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[1]
Bandita Deo, Bikram Pradhan, " In Vitro Apical Culture of Musa Paradisiaca cv. Gaja Bantal, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 5, pp.284-289, September-October-2015.