In Vitro Propagation of Phaius luridus Thwaites - A Terrestrial and Endemic Orchid of Western Ghats

Authors

  • Sr. Sagaya Mary  Plant Tissue Culture Division, Department of Botany, St. Joseph’s Post-Graduate Studies and Research Centre, Langford Road, Bangalore, Karnataka, India
  • Divakar K. M.  Plant Tissue Culture Division, Department of Botany, St. Joseph’s Post-Graduate Studies and Research Centre, Langford Road, Bangalore, Karnataka, India

Keywords:

Phaius luridus Thwaites, PLB’s,MS,VW, B5, BAP, NAA, IAA, CM, Ex Vitro Rooting.

Abstract

Family Orchidaceae constitutes one of the largest families of flowering plants, having around 20,000 species. They are unique in forms, colors and flower structure. The genus Phaius luridus Thwaites is the terrestrial orchid, endemic to the Western Ghats and is an endangered species. A rapid in vitro seed germination technique is described here. MS, VW, B5 and KC media supplemented with various concentrations of auxins and cytokinins were used in combination for asymbiotic seed germination and plantlet formation. In the evaluation of the media MS medium supplemented with 2 mg BAP/L-1+ 5mg NAA/L-1 was found to be suitable with both liquid and solid. Even B5 solid and liquid medium supplemented with 2 mg BAP/L-1+1mg IAA/L-1 was found to be suitable. Further, hormonal concentrations of auxins and cytokinins were evaluated for minimal and optimal levels in the medium. Hardened plants were transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2015-10-25

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Volume 1, Issue 5, September-October-2015

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Research Articles

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[1]
Sr. Sagaya Mary, Divakar K. M., " In Vitro Propagation of Phaius luridus Thwaites - A Terrestrial and Endemic Orchid of Western Ghats , International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 5, pp.356-360, September-October-2015.