IJSRSET calls volunteers interested to contribute towards the scientific development in the field of Science, Engineering and Technology

Home > IJSRSET151583                                                     


In Vitro Propagation of Phaius luridus Thwaites - A Terrestrial and Endemic Orchid of Western Ghats

Authors(2):

Sr. Sagaya Mary, Divakar K. M.
  • Abstract
  • Authors
  • Keywords
  • References
  • Details

Family Orchidaceae constitutes one of the largest families of flowering plants, having around 20,000 species. They are unique in forms, colors and flower structure. The genus Phaius luridus Thwaites is the terrestrial orchid, endemic to the Western Ghats and is an endangered species. A rapid in vitro seed germination technique is described here. MS, VW, B5 and KC media supplemented with various concentrations of auxins and cytokinins were used in combination for asymbiotic seed germination and plantlet formation. In the evaluation of the media MS medium supplemented with 2 mg BAP/L-1+ 5mg NAA/L-1 was found to be suitable with both liquid and solid. Even B5 solid and liquid medium supplemented with 2 mg BAP/L-1+1mg IAA/L-1 was found to be suitable. Further, hormonal concentrations of auxins and cytokinins were evaluated for minimal and optimal levels in the medium. Hardened plants were transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.

Sr. Sagaya Mary, Divakar K. M.

Phaius luridus Thwaites, PLB’s,MS,VW, B5, BAP, NAA, IAA, CM, Ex Vitro Rooting.

  1. Ananda Rao, 1998. Conservation of wild orchids of Kodagu in the Western Ghats. Navabharat Publishers, Seshadripuram, Bangalore.
  2. Arditti. J. 1979. Adv. Bot. Res., 7: 422-638.
  3. Ananda Rao and Sridhar S. 2007 Wild orchids of Karnataka. A Pictorial Compendium. Navabharat Publishers, Seshadripuram, Bangalore.
  4. Bhagabati A K, Kalita M C & Baruah S. 2006. Biodiversity of Assam. Assam Science Society, Guwahati, Assam, India.
  5. Chowdhery H J. 2009. Orchid Diversity in North-Eastern States. in: J Orchid Soc. India, 23 (1-2): 17-25.
  6. Chowdhery S. 2005. Assam’s Flora. Assam Science Technology and Environment Council, Guwahati, Assam, India.
  7. Chyuam-Yih Ng. and Norihan Mohd. S., In vitro propagation of Paphiopedilum orchid through formation of protocormlike bodies, Plant Cell. Tissue and Org. Cult., 105: 193-202, (2011).
  8. Deb C.R. and Temjensangba, In vitro propagation of threatened terrestrial orchid, Malaxis khasiana Soland ex. Swartz through immature seed culture.
  9. Dutra D., Johnson T.R., Kauth P.J., Stewart S.L., Kane M.E. and Richardson L., Asymbiotic seed germination, in vitro seedling development and greenhouse acclimatization of the threatened terrestrial, orchid Bletia purpurea, Plant Cell Tiss Organ Cult., 94: 11–21, (2008).
  10. Duncan D.B., Multiple range and multiple f- tests, Biometrics, 11: 1-42, (1955).
  11. Dix L. and Van Staden J., Auxin and gibberellins-like substances in coconut milk and malt extract, Plant Cell Tissue and Org. Cult., 1: 239–245, (1982).
  12. Gonçalves S., Martins N. and Romano A., Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis, Biol. Plantarum, 53: 774-778, (2009).
  13. Hosomi S.T., Santos R.B., Custodio C.C., Seaton P.T., Marks T.R. and Machado-Neto N.B., Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability, Seed Science and Technology, 39: 178-189, (2011).
  14. Kalyan Kumar De. 1992. Plant tissue Culture.
  15. Khyanjeet Gogoi, Raju Das, Rajendra Yonzone (2012). Phaius luridus Thwaites (Orchidaceae): a new record for Assam, India. Science Research Reporter 2(3):295-297, Oct. 2012. ISSN: 2249-2321
  16. Knudson L (1921). Bull. Real, Sa. Espan’ola Hist. Nut. 21:25C260.
  17. Kumar Sathish & Manilal K S. 1994. A Catalogue of India Orchids. Bishen Singh Mahendral Pal Singh, Dehra Dun, India.
  18. Lakshmanan P., Loh C.S. and Goh C.J., An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture, Plant Cell Rep., 14: 510–514, (1995).
  19. Misra S. 2007. Orchids of India. Bishen Singh Mahendra Pal Singh, Dehra Dun, India.
  20. Murashige T. and Skoog F., A revised medium for rapid growth and bioassays with tobacco tissues cultures, Physiol. Plant, 15: 473-497, (1962).
  21. Mauney J.R., Hillman W.S., Miller C.O. and Skoog M.F., Bioassay, purification and properties of a growth factor from coconut. Physiol. Plantarum, 5: 485–479, (1952).
  22. Paek K.Y., Hahn E.J. and Park S.Y., Micropropagation of Phalaenopsis Orchids via Protocorms and ProtocormLike Bodies, 71: 293-306. In: Thorpe, Trevor A.; Yeung, Edward C. (Eds.), Plant Embryo Culture: Methods and Protocols, Series- Methods in Molecular Biology: (2011).
  23. Rajput S.R. and Bora P.S., Quinazolin-4ones as antifungal agents, Intern. J. of Pharma and Biosciences, 3 (4): 119- 132, (2012).    
  24. Singh F., Differential staining of orchid seeds for viability testing, Am. Orchid Soc. Bull., 50: 416-418, (1981).
  25. Teo C.K.H., Kunisaki J.T. and Sagawa Y., Clonal propagation of strap - leafed Vanda by shoot - tip culture, Am. Orchid Soc. Bull., 42: 402-405, (1973).
  26. This article can be downloaded from www.ijpbs.net B - 486

Publication Details

Published in : Volume 1 | Issue 5 | September-October - 2015
Date of Publication Print ISSN Online ISSN
2015-10-25 2395-1990 2394-4099
Page(s) Manuscript Number   Publisher
356-360 IJSRSET151583   Technoscience Academy

Cite This Article

Sr. Sagaya Mary, Divakar K. M., "In Vitro Propagation of Phaius luridus Thwaites - A Terrestrial and Endemic Orchid of Western Ghats ", International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 5, pp.356-360, September-October-2015.
URL : http://ijsrset.com/IJSRSET151583.php

IJSRSET Xplore

Subscribe

Conferences

National Conference on Advances in Mechanical Engineering 2017(NCAME 2017)

National Conference on Emerging Trends in Civil Engineering 2017( NCETCE 2017)