Manuscript Number : IJSRSET151591

Developments of HPLC Method for Determination of Methyl Dopa

Authors(1) :-Hussein Hassan Kharnoob

This research  contains  the development of accurate and quick method for determination  methyldopa drug using high performance  liquid  chromatographic technique, injected 20 μl of the drug solution with concentration 20 μg/ml using a column type C18 , mobile phase consists of  Acetonitrile: 0.02M KH2PO4 (70 : 30) at flow rate of 1.6 m1/min using UV detector at 275nm, pH5.65 ,with retention time 2.89min at linear range 10 – 50μg/ml with good accuracy and precision . The recovery value is to 99.2%and detection limit of 0.05 μg/ ml. The method is applied successfully to determine methyl dopa in its pharmaceutical formulations.

Authors and Affiliations

Hussein Hassan Kharnoob
College Pharmacy, Tikrit University, Tikrit, Iraq

Chromatography, Methy Dopa, Acetonitrile, Drug

  1. The Merck Index , 12th Copyright by Merck Co. , Inc. Whiteho , 2000,  CD Rom
  2. Clarke' , "Analysis of Drugs and Poisons ", 3nd , p1252 – 1253, 2006.
  3. M. A. Ali, M. S. Ali and  A. M. K. M. Yar, International Journal of Chemical & Environmental Engineering, 4 (3) , 159, 2013.
  4. S. Ting ,"   J Assoc Off Anal Chem.,66(6), 1436 - 1442 ,  1983.                                                      
  5. M. Dolezalova and M. Tkacykova , J Pharm Biomed Anal. , 19(3-4) , 555 – 567, 1999.
  6. G.M. Kochak and W.D. Mason , Journal of Pharmaceutical Sciences , 69 (8), 897 – 900, 1980.
  7. G. Bahrami, A. Kiani and S. Mirzaeei , Journal of Chromatography B , 832(2), 197 – 201, 2006.
  8. A. Majidano, N. A. Sodho, G. N. Rajper, M. A. Alamgir and M. Y. Khuhawar, Sindh Univ. Res. Jour. (Sci. Ser.) , 46 (1) , 77 - 82  , 2014.
  9. M. V.L. Sahithi, V. Perumal, K. Sekhar, and P. Vijay Babu, journal of Advanced pharmacy Education & Researh, 3 (4), 464 – 470 , 2013.
  10. G.Aymard,M.Legrand,N.Trichereau and B. Diquet., J. of Chromatography B: Biomedical Sciences and Applications,744 (2) , 227-240 ,2000.
  11. A . A . AL-Daamy and  F. AL-Moswi,Kerbala Journal of Pharmaceutical Sciences , 6  , 70 – 77 , 2013.
  12. E. A. Gadkariem, K. E. E. Ibrahim,N. A. A. Kamil,M. E. M. Haga, and H. A.  El-Obeid, Saudi Pharm J, 17 , ( 4 ) , 289 – 293 , 2009.
  13. P. R. S. Ribeiro; L. Pezza and H. R. Pezza, Eclet . Quim , 30( 3 ) , 23 - 28 , 2005.
  14. R. O. Matos, C. F. Silva and R. R. Silva , Latin American Journal of Pharmacy , 31 ( 2 ), 190 –1 94, 2002 .
  15. M. A. Gotardo, L. S. Lima, R. Sequinel, J. L. Rufino, L. Pezza, and H. R. Pezza Ecl. Quim., Sao Paulo,  33(3), 7-12 , 2008.
  16. M. A.A. Daamy and R. F. AL- Moswi, Asian Journal of  Chemistry , 14(2) , 867 – 873, 2002.
  17. M. A. Gotardo, L. S. Lima, R. Sequined, J. L. Rufino, L. Pezza and  H. R. Pezza,"Ecl. Quim., Sao Paulo, 33(3): 7-12, 2008.
  18. Brynn Hibbert, D., New York , USA , 2007, p 254
  19. I. T. Haoon, University of Tikrit, College of Science, Thkrit J. for pharmacy,(38)  178, 2014.

Publication Details

Published in : Volume 1 | Issue 6 | November-December 2015
Date of Publication : 2015-12-25
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 22-28
Manuscript Number : IJSRSET151591
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Hussein Hassan Kharnoob, " Developments of HPLC Method for Determination of Methyl Dopa, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 6, pp.22-28, November-December-2015.
Journal URL : http://ijsrset.com/IJSRSET151591

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