In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp.

Authors(3) :-Meriem Djekoun, Houria Berrebbah, Mohammed Reda Djebar

The freshwater protozoan ciliate Paramecium sp. was used in order to assess the potential cytotoxic effects of Thiram, a dithiocarbamates fungicide. To estimate the impact of this fungicide, cells were exposed to three concentrations (0.001, 0.002 and 0.003 mg/l) for 4 days. The respiratory metabolism and the antioxidant defence biomarkers (Catalase and reduced Glutathione) were assessed. Acute exposure caused marked decrease in number of cells with EC50 value estimated at 0.0028 mg/l. The obtained results concerning the cellular respiratory reveal a dose-dependent perturbation in exposed organisms. Also, the biomarkers assessed were sensitive to the presence of Thiram wich induced a strong antioxidative activities by stimulating the enzymatic activity of Catalase (CAT) and decreasing the rate of reduced Glutathione (GSH).

Authors and Affiliations

Meriem Djekoun
Department of Biology, Badji Mokhtar University, Laboratory of Cellular Toxicology, Annaba, Algeria
Houria Berrebbah
Department of Biology, Badji Mokhtar University, Laboratory of Cellular Toxicology, Annaba, Algeria
Mohammed Reda Djebar
Department of Biology, Badji Mokhtar University, Laboratory of Cellular Toxicology, Annaba, Algeria

Paramecium sp., Thiram, Cytotoxicity, EC50, Biomarkers

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Publication Details

Published in : Volume 1 | Issue 6 | November-December 2015
Date of Publication : 2015-12-25
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 320-326
Manuscript Number : IJSRSET151640
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Meriem Djekoun, Houria Berrebbah, Mohammed Reda Djebar, " In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp., International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 6, pp.320-326, November-December-2015.
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