In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp.

Authors

  • Meriem Djekoun  Department of Biology, Badji Mokhtar University, Laboratory of Cellular Toxicology, Annaba, Algeria
  • Houria Berrebbah  Department of Biology, Badji Mokhtar University, Laboratory of Cellular Toxicology, Annaba, Algeria
  • Mohammed Reda Djebar  Department of Biology, Badji Mokhtar University, Laboratory of Cellular Toxicology, Annaba, Algeria

Keywords:

Paramecium sp., Thiram, Cytotoxicity, EC50, Biomarkers

Abstract

The freshwater protozoan ciliate Paramecium sp. was used in order to assess the potential cytotoxic effects of Thiram, a dithiocarbamates fungicide. To estimate the impact of this fungicide, cells were exposed to three concentrations (0.001, 0.002 and 0.003 mg/l) for 4 days. The respiratory metabolism and the antioxidant defence biomarkers (Catalase and reduced Glutathione) were assessed. Acute exposure caused marked decrease in number of cells with EC50 value estimated at 0.0028 mg/l. The obtained results concerning the cellular respiratory reveal a dose-dependent perturbation in exposed organisms. Also, the biomarkers assessed were sensitive to the presence of Thiram wich induced a strong antioxidative activities by stimulating the enzymatic activity of Catalase (CAT) and decreasing the rate of reduced Glutathione (GSH).

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2015-12-25

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Volume 1, Issue 6, November-December-2015

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Research Articles

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[1]
Meriem Djekoun, Houria Berrebbah, Mohammed Reda Djebar, " In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp., International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 6, pp.320-326, November-December-2015.