IJSRSET calls volunteers interested to contribute towards the scientific development in the field of Science, Engineering and Technology

Home > IJSRSET151640                                                     


In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp.

Authors(3):

Meriem Djekoun, Houria Berrebbah, Mohammed Reda Djebar
  • Abstract
  • Authors
  • Keywords
  • References
  • Details

The freshwater protozoan ciliate Paramecium sp. was used in order to assess the potential cytotoxic effects of Thiram, a dithiocarbamates fungicide. To estimate the impact of this fungicide, cells were exposed to three concentrations (0.001, 0.002 and 0.003 mg/l) for 4 days. The respiratory metabolism and the antioxidant defence biomarkers (Catalase and reduced Glutathione) were assessed. Acute exposure caused marked decrease in number of cells with EC50 value estimated at 0.0028 mg/l. The obtained results concerning the cellular respiratory reveal a dose-dependent perturbation in exposed organisms. Also, the biomarkers assessed were sensitive to the presence of Thiram wich induced a strong antioxidative activities by stimulating the enzymatic activity of Catalase (CAT) and decreasing the rate of reduced Glutathione (GSH).

Meriem Djekoun, Houria Berrebbah, Mohammed Reda Djebar

Paramecium sp., Thiram, Cytotoxicity, EC50, Biomarkers

  1. Amamra R., Djebar M.R., Grara N., Moumeni O., Otmani H., Alayat A., and Berrebbah H. 2015. Cypermethrin-Induces Oxidative Stress to the Freshwater Ciliate Model: Paramecium tetraurelia. Ann. Res. & Rev. Biol. 5(5): 385-399.
  2. Amanchi N. and Bhagavathi M. 2009. Comparative study on cytotoxicity of delfin insecticide using two vital protozoan ciliates Paramecium caudatum and Oxytricha fallax. Asian J. Exp. Sci. 23: 55-60.
  3. Amanchi N.R. and Hussain M.M. 2010. Cytotoxicity assessment of monocrotophos in Paramecium caudatum and Oxytricha fallax. J. Environ. Biol. 31(5): 603-607.
  4. Apostol S., 1973. A Bioassay of Toxicity Using Protozoa in the Study of Aquatic Environment Pollution and Its Prevention. Environ. Res. 6: 365-372 .
  5. Azzouz Z., Berrebbah H., Djebar M.R. 2011. Optimization of Paramecium tetraurelia growth kinetics and its sensitivity to combined effects of azoxystrobin and cyproconazole. African J. Microbiol. Res. 5(20): 3243-3250.
  6. Bashandy T., Guilleminot J., Vernoux T., Caparros-Ruiz D., Ljung K., Meyer Y., Reichheld J.P. 2010. Interplay between the NADP-linked thioredoxin and glutathione systems in Arabidopsis auxin signaling. Plant Cell. 22: 376-391.
  7. Benbouzid H., Berrebbah H., Berredjem M., Djebar M.R. 2012. Toxic effects of phophoramidate on Paramecium sp. With special emphasis on respiratory metabolism, growth and generation time. Toxicol. Environ. Chem. 94 (3): 557-565.
  8. Benbouzid H., Berrebbah H., Djebar M.R. 2015. Toxicity of the Chlorfenapyr: Growth inhibition and induction of oxidative stress on a freshwater protozoan: Paramecium sp. Adv. Environ. Biol. 9(3): 281-285.
  9. Bluzat R., Jonot O., Seugi J. 1982. Toxicité aigue d'un fongicide, le thirame (dithiocarbamate), chez le crustacé amphipode d'eau douce Gammarus pulex. Environ. Pollut. 29, 225-233.
  10. Bonnemain H. and Dive D. 1990. Studies on Synergistic Toxic Effects of Copper and Dithiocarbamate Pesticides with the Ciliate Protozoan Colpidium campylurn (Stokes). Ecotox. Environ. Safe. 19: 320-326.
  11. Clasen B., Leitemperger J., Murussi C., Pretto A., Menezes C., Dalabona F., Marchezan E., Bohrer Adaime M., Zanella R., Loro V.L. 2014. Carbofuran promotes biochemical changes in carp exposed to ricefield and laboratory conditions. Ecotox. Environ. Safe. 101: 77-82.
  12. De Silva H.J., Samarawickrema N.A., Wickremasinghe A.R. 2006. Toxicity due to organophosphorus compounds: what about chronic exposure? Trans. R. Soc Trop Med Hyg. 100: 803-806.
  13. Dewez D., Geoffroy L., Vernet G. Popovic R. 2005. Determination of photosynthetic and enzymatic biomarkers sensitivity used to evaluate toxic effects of copper and fludioxonil in alga Scenedesmus obliquus. Aquat. Toxicol. 74: 150-159.
  14. Djebar H. and Djebar M.R. 2000. Bioénergétique, Les mitochondries végétales. Revue des sciences et technologie ; Synthèse 2000. Publication de l’Université de Annaba. Edition Végarol: 103.
  15. Djekoun M., Berrebah H. and Djebar M.R. 2015. Growth kinetics, monitoring of biomarkers and respiratory metabolism to evaluate the combined effects of fludioxonil and difenoconazole in a freshwater ciliated protist: Paramecium sp. Der Pharma Chemica. 7(11): 197-204.
  16. Finlay B.J. and Fenchel T. 2004 Cosmopolitan metapopulations of free-living eukaryotes. Protist. 155: 237-244.
  17. Fleeger, J.W., Carman, K.R., Nisbet, R.M., 2003. Indirect effects of contaminants in aquatic ecosystems. Sci. Total Environ. 317: 207-233.
  18. Foyer C. and Noctor G. 2005. Oxidant and antioxidant signalling in plants: A re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell. Environ. 28:1056-1071.
  19. Franekic J., Bratulic N., Pavlica M., Papes D. 1994. Genotoxicity of dithiocarbamates and their metabolites. Mutat. Res. 325: 65–74.
  20. Gimeno-Garcia E., Andreu V., Boluda R. 1996. Heavy metals inci-dence in the application of inorganic fertilizers and pesticides to rice farming soils. Environ. Pollut. 92 : 19-25.
  21. Ledin M., 2000. Accumulation of metals by microorganisms-processes and importance for soil systems. Earth-Sci. Rev. 51: 1-31.
  22. Lin L.L., Janz D.M. 2006. Effects of binary mixtures of xenoestrogens on gonadal developement and reroduction in zebrafish. Aquat. Toxicol. 80: 382-395.
  23. Liu L., Zhu B., Gong Y., Liu G., Wang G., 2015. Neurotoxic effect of triazophos on gold fish (Carassius auratus) and tissue specific antioxidant responses. Ecotoxicol. Environ. Saf. 116: 68-75.
  24. Maksymiec W., 1997. Effect of copper in higher plants. Photosynthetica. 34: 321-342.
  25. Mittler R., Vanderauwera S., Gollery M., Van Breusegem F. 2004. Reactive oxygen gene network of plants. Trends Plant. Sci. 9: 490-498.
  26. Miyoshi N., Kawano T., Tanaka M., Kadono T., Kosaka T., Kunimoto M., Takahashi T., Hosoya H. 2003. Use of paramecium species in bioassays for environmental risk management: determination of IC50 values for water pollutants, J. Health Sci. 49: 429-435.
  27. Mountassif D., Kabine M., Manar R., Bourhim N., Zaroual Z. Latruffe N., El Kebbaj M.S. 2007. Physiological, morphological and metabolic changes in Tetrahymena pyriformis for the in vivo cytotoxicity assessment of metallic pollution: Impact on D-b-hydroxybutyrate dehydrogenase. Ecol. Ind. 7: 882-894.
  28. Rao J.V., Srikanth K., Arepalli S.K., Gunda V.G., Toxic effects of acephate on Paramecium caudatum with special emphasis on morphology, behaviour, and generation time, Pest. Biochem. Physiol. 86: 131-137.
  29. Regoli F and Principato G. 1995. Glutathione, glutathione-dependant and antioxidant enzymes in mussel Mytilus galloprovincialis exposed to metals under field and laboratory conditions: implication for the biomarkers. Aquat. Toxicol. 31: 143-164.
  30. Regoli F., Gorbi S., Frenzilli G., Nigro M., Corsi I., Focardi S., Winston G.W., 2002a. Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach. Mar. Environ. Res. 54: 419–423.
  31. Regoli F., Pellegrini D., Winston G.W., Gorbi S., Giuliani S., Virno-Lamberti C., Bompadre S., 2002b. Application of biomarkers for assessing the biological impact of dredged materials in the Mediterranean: the relationship between antioxidant responses and susceptibility to oxidative stress in the red mullet (Mullus barbatus). Mar. Pollut. Bull. 44: 912-922.
  32. Rehman A., Shuja R. and Shakoori A.R. 2008. Tolerance of Two Ciliates, Stylonychia mytilusand, Paramecium caudatum, Isolated from Industrial Effluents to an Organophosphate, Endosulfan and their Potential use in Bioremediation of Insecticide-contaminated Wastewater. Pakistan J. Zool.40(4): 255-261.
  33. Robinson P.W. 1998. The toxicity of pesticides and organics to mysid shrimps can be predicted from Daphnia spp. Toxicity data. Wat. Res. 33 (6): 1545-1549.
  34. Rouabhi R, Berrebbah H, Djebar MR. 2006. Toxicity evaluation of flucycloxuron and diflubenzuron on the cellular model, Paramecium sp. Afr. J. Biotechnol. 5 (1): 45-48.
  35. Rouabhi R. 2011. Influence of Nickel on Growth, Movement Speed, Respiratory Metabolism, and Biochemical Parameters of Paramecium sp. WebMed Central. 2046-1690.
  36. Saib, A., Berrebbah H., Berredjem M., Djebar M. R. 2014. Cytotoxic study of three derivatives amidophosphonates on alternative cellular model: Paramecium tetraurelia. Toxicol. Res. 3: 395-399.
  37. Sancho E., Villarroel M.J., Andreu E., Ferrando M.D. 2009. Disturbances in energy metabolism of Daphnia magna after exposure to tebuconazole. Chemosphere. 74: 1171-1178.
  38. Sunil S., Rashi G., Shilpi S. 2015. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiate. J. Hazard. Mater. 291: 102-110.
  39. Vindimian E., Robaut C. and Fillion G. 1983. A method for cooperative and non cooperative binding studies using non linear regression analysis on a microcomputer. J. Appl. Biochem. 5: 261-268.
  40. Wan M.T., Watts R.G., Moul D.J. 1994. Impact of chemigation on selected non-target aquatic organisms in cranberry bogs of British Columbia, Bull. Environ. Contam. Toxicol. 53: 828-835.
  41. Wechbeker G, Cory J.G. 1988. Ribonucleotide reductase activity and growth of glutathione depleted mouse leukemia L1210 cells in vitro. Cancer let. 40: 257-264.
  42. Xiyan M., Sen P., Xingze S., Jiejun G., Jiayi C., Xiaofeng C., Xuefeng L., Chengju W. 2013. Evaluation of acute and developmental effects of difenoconazole via multiple stage zebrafish assays. Environ. Pollut. 175 : 147-157.
  43. Yonar S., Ural, M., Silici, S., Yonar, M., 2014. Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: protective role of propolis. Ecotoxicol. Environ. Saf. 102: 202-209.

Publication Details

Published in : Volume 1 | Issue 6 | November-December - 2015
Date of Publication Print ISSN Online ISSN
2015-12-25 2395-1990 2394-4099
Page(s) Manuscript Number   Publisher
320-326 IJSRSET151640   Technoscience Academy

Cite This Article

Meriem Djekoun, Houria Berrebbah, Mohammed Reda Djebar, "In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp.", International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 6, pp.320-326, November-December-2015.
URL : http://ijsrset.com/IJSRSET151640.php

IJSRSET Xplore

Subscribe

Conferences

National Conference on Advances in Mechanical Engineering 2017(NCAME 2017)

National Conference on Emerging Trends in Civil Engineering 2017( NCETCE 2017)