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Preparation and SPE applications of silica-based monoliths


Eman Alzahrani
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The monolithic column is a single piece of a continuous rigid porous polymer that possess an interconnected skeletal structure and contains pores. According to their components, monolithic materials can be divided into organic polymer-based monoliths and inorganic silica-based monoliths. This review focuses on silica-based monolith with special emphasis on the preparation. Moreover, this review attempts to give an overview covering the modification of the surface of the inorganic monolithic materials. The applications of the silica-based monoliths as a sorbent in different applications are included in this review.

Eman Alzahrani

Silica-based monolith; Modification the surface; Applications; Review.


 [1] Frantisek, S., Less common applications of monoliths: Preconcentration and solid-phase extraction. Journal of Chromatography B, 2006. 841(1–2): p. 52-64.

[2] Lion, N., et al., Why the move to microfluidics for protein analysis? Current Opinion in Biotechnology, 2004. 15(1): p. 31-37.

[3] Lin, Z., et al., Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography. Journal of Chromatography A, 2009. 1216(49): p. 8612-8622.

[4] Gambero, A., et al., Use of Chemically Modified Silica with β-Diketoamine Groups for Separation of α-Lactoalbumin from Bovine Milk Whey by Affinity Chromatography. Journal of Colloid and Interface Science, 1997. 185(2): p. 313-316.

[5] Cohen, S.L. and B.T. Chait, Influence of Matrix Solution Conditions on the MALDI-MS Analysis of Peptides and Proteins. Analytical Chemistry, 1996. 68(1): p. 31-37.

[6] Siouffi, A.M., Silica gel-based monoliths prepared by the sol–gel method: facts and figures. Journal of Chromatography A, 2003. 1000(1–2): p. 801-818.

[7] Fields, S.M., Silica Xerogel as a Continuous Column Support for High-Performance Liquid Chromatography. Analytical Chemistry, 1996. 68(15): p. 2709-2712.

[8] Zou, H., et al., Monolithic stationary phases for liquid chromatography and capillary electrochromatography. Journal of Chromatography A, 2002. 954(1–2): p. 5-32.

[9] Alzahrani, E., Fabrication of Monolithic Silica Microchip for Efficient DNA Purification. International Journal of Advanced Engineering and Nano Technology, 2014. 2(13-18).

[10] 10. Minakuchi, H., et al., Octadecylsilylated Porous Silica Rods as Separation Media for Reversed-Phase Liquid Chromatography. Analytical Chemistry, 1996. 68(19): p. 3498-3501.

[11] Lee, S.H., et al., Influence of ionic liquids as additives on sol–gel immobilized lipase. Journal of Molecular Catalysis B: Enzymatic, 2007. 47(3–4): p. 129-134.

[12] Kato, M., K. Sakai-Kato, and T. Toyo'oka, Silica sol-gel monolithic materials and their use in a variety of applications. Journal of Separation Science, 2005. 28(15): p. 1893-1908.

[13] Ishizuka, N., et al., Monolithic silica columns for high-efficiency separations by high-performance liquid chromatography. Journal of Chromatography A, 2002. 960(1-2): p. 85-96.

[14] Frolova, A.M., et al., Producing of Monolithic Layers of Silica for Thin-Layer Chromatography by Sol-Gel Synthesis. The Open Surface Science Journal 2009. 1: p. 40-45.

[15] Adam, T., et al., Towards the column bed stabilization of columns in capillary electroendosmotic chromatography: Immobilization of microparticulate silica columns to a continuous bed. Journal of Chromatography A, 2000. 887(1-2): p. 327-337.

[16] Xu, L., Z. Shi, and Y. Feng, Porous monoliths: sorbents for miniaturized extraction in biological analysis. Analytical and  Bioanalytical Chemistry, 2010. 399(10): p. 3345-3357.

[17] Allen, D. and Z. El Rassi, Capillary electrochromatography with monolithic silica column: I. Preparation of silica monoliths having surface-bound octadecyl moieties and their chromatographic characterization and applications to the separation of neutral and charged species. Electrophoresis 2003. 24: p. 408–420.

[18] Keeling-Tucker, T., et al., Controlling the Material Properties and Biological Activity of Lipase within Sol−Gel Derived Bioglasses via Organosilane and Polymer Doping. Chemistry of Materials, 2000. 12(12): p. 3695-3704.

[19] Siouffi, A., Silica gel-based monoliths prepared by the sol-gel method: facts and figures. Journal of Chromatography A, 2003. 1000(1-2): p. 801-818.

[20] Rieux, L., et al., Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules. Journal of Separation Science, 2005. 28(14): p. 1628-1641.

[21] Simpson, N., Solid-Phase Extraction: Principles, Techniques, and Applications 2000, New York: Marcel Dekker.

[22] Sandoval, J.E. and J.J. Pesek, Hydrolytically stable bonded chromatographic phases prepared through hydrosilylation of olefins on a hydride-modified silica intermediate. Analytical Chemistry, 1991. 63(22): p. 2634-2641.

[23] Herrero-Martı́nez, J.M., et al., Characterization of the acidity of residual silanol groups in microparticulate and monolithic reversed-phase columns. Journal of Chromatography A, 2004. 1060(1–2): p. 135-145.

[24] Sanagi, M., et al., Preparation and Application of Octadecylsilyl-Silica Adsorbents for Chemical Analysis. Malaysian Journal of Analytical Sciences, 2001. 7: p. 337-343.

[25] Thurman, E.M. and M.S. Mills, Solid-Phase Extraction: Principles and Practice. 1998, New York: Wiley.

[26] Larrivee, M.L. and C.F. Poole, Solvation parameter model for the prediction of breakthrough volumes in solid-phase extraction with particle-loaded membranes. Analytical Chemistry, 1994. 66(1): p. 139-146.

[27] Girault, H., et al., Why the move to microfluidics for protein analysis. Current Opinion in Chemical Biology, 2004. 15( 1): p. 31-37.

[28] Kelea, M. and G. Guiochon, Repeatability and reproducibility of retention data and band profiles on six batches of monolithic columns. J. Chromatogr., A, 2002. 960: p. 19–49.

[29] Hennion, M.C., Solid-phase extraction: method development, sorbents, and coupling with liquid chromatography. J. Chromatogr.,  A, 1999. 856: p. 3–54.

[30] Jal, P.K., S. Patel, and B.K. Mishra, Chemical modification of silica surface by immobilization of functional groups for extractive concentration of metal ions. Talanta, 2004. 62(5): p. 1005-1028.

[31] Guiochon, G., Monolithic columns in high-performance liquid chromatography. Journal of Chromatog. A, 2007. 1168: p. 101-168.

[32] Alzahrani, E. and K. Welham, Fabrication of an octadecylated silica monolith inside a glass microchip for protein enrichment. Analyst, 2012. 137(20): p. 4751-4759.

[33] Poole, C.F., New trends in solid-phase extraction. TrAC Trends in Analytical Chemistry, 2003. 22(6): p. 362-373.

[34] Luo, Q., et al., Preparation of 20-μm-i.d. Silica-Based Monolithic Columns and Their Performance for Proteomics Analyses. Analytical Chemistry, 2005. 77(15): p. 5028-5035.

[35] Ota, S., et al., High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula. Journal of Biochemical and Biophysical Methods, 2007. 70(1): p. 57-62.

[36] Nema, T., E. Chan, and P. Ho, Application of silica-based monolith as solid phase extraction cartridge for extracting polar compounds from urine. Talanta, 2010. 82(2): p. 488-494.

[37] Wolfe, K.A., et al., Toward a microchip-based solid-phase extraction method for isolation of nucleic acids. Electrophoresis      2002. 23(5): p. 727-733.

[38] Miyazaki, S., et al., Development of a monolithic silica extraction tip for the analysis of proteins. Journal of Chromatography A, 2004. 1043(1): p. 19-25.

[39] Wu, Q., et al., Microchip-Based Macroporous Silica Sol-Gel Monolith for Efficient Isolation of DNA from Clinical Samples. Anal. Chem. , 2006. 78: p. 5704-5710.

[40] Xu, L. and H.K. Lee, Preparation, characterization and analytical application of a hybrid organic–inorganic silica-based monolith. Journal of Chromatography A, 2008. 1195(1–2): p. 78-84.


Publication Details

Published in : Volume 1 | Issue 2 | March-April - 2015
Date of Publication Print ISSN Online ISSN
2015-04-25 2395-1990 2394-4099
Page(s) Manuscript Number   Publisher
138-143 IJSRSET152218   Technoscience Academy

Cite This Article

Eman Alzahrani, "Preparation and SPE applications of silica-based monoliths ", International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue 2, pp.138-143, March-April-2015.
URL : http://ijsrset.com/IJSRSET152218.php