A Study on the Effect of Preservation on the Microbial Growth in Fruit Juices

Authors(2) :-Hajisha Nair, Yeturi Gayatri

Traditionally fruit juices and fruit products are considered as microbiologically safer than other unprocessed foods. Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play an important role in the prevention of heart diseases, cancer, and diabetes. The objective of this study is to evaluate microbiological safety and quality of freshly prepared juices and preserved juices. Thirty fruit juice samples were collected from different areas in and around Hyderabad .Some of the packed juice products were also collected for the study. The microorganisms isolated from both fresh street vended juices and packed liquid products were identified as Staphylococcus spp, Lactobacillus spp, Cornybacterium spp, Micrococcus spp, Pseudomonas spp by biochemical tests. Pathogens like Cornybacterium, Staphylococcus were also isolated. On preservation of fruit juices for 5 days the microbial load increased when compared to fresh juice samples. The viable count of sample increases from 4 x 100 to 610 x 100 CFU/ml. The Antimicrobial susceptibility testing is performed to check the sensitivity of the isolated pathogenic organisms and it was observed that Staphylococcus aureus was sensitive to all the antibiotics while cornybacterium spp was sensitive to few antibiotics like Gentamicin, Roxythromycin, Cefadroxil, and Azithromycin and resistant to Amikacin, Ampiclox, Ciprofloxacin, Clarithromicin, Cefotaxime, Sparfloxacin.

Authors and Affiliations

Hajisha Nair
Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad, Telangana, India
Yeturi Gayatri
Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad, Telangana, India

Fresh juices, Packed juices, Preservation

  1. M AbulBasar and SabitaRezwanaRahman "Assessment of Microbiological Quality of Processed Fruit Juice” Bangladesh J Microbiol, Volume 24, Number 2, December 2007, 166-168
  2. Kamal Rai Aneja, Romika Dhiman, Neeraj Kumar Aggarwal,Vikas Kumar, and Manpreeet Kaur ” Microbes Associated with Freshly Prepared Juices of Citrus and Carrots” International Journal of Food Science Volume 2014,International journel of food science, 2014; volume 2014
  3. Rashed, N,Md. Aftab, U, Mrityunjoy, A. and Md. Azizul, H., M.Majibur, R.Saurab, K. M. "Microbiological study of vendor and packed fruit juices locally available in Dhaka city, Bangladesh” International Food Research Journal 20(2): 1011-1015 (2013)
  4. Identification flow charts,Bergey’s Manual of Determinative Bacteriology.
  5. Durgesh P. MahaleRanjana G. Khade, Varsha K. Vaidya "Microbiological Analysis of Street Vended Fruit Juices from Mumbai City, India” Internel Journal of Food Safety, Vol.10, 2008, p31-34
  6. Lewis JE, Thompson P, Rao BVVBN, Kalavati C and Rajanna B. 2006.Human bacteria in street vended fruit juices: A case study of Visakhapatnam City, India.Internet Journal of Food Safety. 8:35-38.
  7. Al-Jedah JH and Robinson RK. 2002. Nutritional Value and Microbiological Safety of Fresh Fruit Juices sold through Retail Outlets in Qatar. Pakistan Journal of Nutrition. 1 (2): 79-81.
  8. Harrigan WF. 1998. Laboratory Methods in Food Microbiology. Academic Press London.
  9. Splittstosser DF. 1979. Fruits and Fruit Products. In: Food & Beverage Mycology. Ed. Beuchat, LR. Avi Publishing Co. Inc, Westport, Connecticut.
  10. Sharma "Bacteriological analysis of street vended fruit juicesavailable in Vidarbha" International Journel of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 2 Number 5 (2013) pp. 178-183
  11. Ashok Kumar, VarunBhushan, ShikhaVerma, GauravSrivastav and Sushil Kumar "Isolation and Characterization of Microorganisms Responsible for Different Types of Food Spoilages”International Journal of Research in Pure and Applied Microbiology 2011;1(2):22-31
  12. Santosham, Mathuram; Chan, Grace J.; Lee, Ann CC; Baqui, Abdullah H.; Tan Jingwen; Black, Robert E.(2013). "Risk of Early- Onset Neonatal Infection with Maternal Infection or Colonization: A Global Systemic Review and Meta Analysis”. PLoS Medicine 10(8) 1001502. Doi:10.1371/journel.pmed.1001502.ISSN 1549-1676, PMC 3747995, PMID 23976885
  13. Wang ZK, Yang YS, Stefka AT, Sun G, Peng LH (April 2014). "Review article: fungal microbiota and digestive diseases". Aliment. Pharmacol. Ther. 39 (8): 751–766. In addition, GI fungal infection is reported even among those patients with normal immune status. Digestive system-related fungal infections may be induced by both commensal opportunistic fungi and exogenous pathogenic fungi. In vitro, bacterial hydrogen peroxide or organic acids can inhibit C. albicans growth and virulence In vivo, Lactobacillus sp. can inhibit the GI colonisation and infection of C. albicans In vivo, C. albicans can suppress Lactobacillus sp. regeneration in the GI tract after antibiotic therapy
  14. Inglin, Raffael C. (2015). "High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species". Journal of Microbiological Methods. 114 (July 2015): 26–29.
  15. Abbott, S. L.; Janda, J. M. (2006). "The Genus Edwardsiella". Prokaryotes 6: 72–89
  16. E. Giddens, JR. K.K. Keahey, G.R Carter and C.K. Whitehair " Pneumonia in Rats Due to Infection with Corynebacterium kutscheri " Path. vet. 5: 227-237 (1968)
  17. Lortholory, A.Buu-Hoi, J.Y. Fagon, J. Pierre, M. Slamma, L. Gutmann, and J.F Acar " Mediastinitis due to multiply resistant Cornybacterium xerosis" Clinical infectious disease 1993;16:172

Publication Details

Published in : Volume 2 | Issue 4 | July-August 2016
Date of Publication : 2016-08-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 577-587
Manuscript Number : IJSRSET1624121
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Hajisha Nair, Yeturi Gayatri, " A Study on the Effect of Preservation on the Microbial Growth in Fruit Juices, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 2, Issue 4, pp.577-587, July-August-2016.
Journal URL : http://ijsrset.com/IJSRSET1624121

Article Preview