In Vitro, Optimization of Antibacterial Activity of Secondary Metabolites Produced By Aspergillus Flavus (Mtcc-3396)

Authors

  • Kalyani. P  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India
  • Geetha. S  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India
  • Hemalatha. K. P. J  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India

Keywords:

Aspergillus flavus (MTCC-3396), Metabolite production, Optimization.

Abstract

The purpose of the present study was to investigate the influence of cultural conditions and environmental parameters affecting the growth and bioactive metabolite production of the fungi Aspergillus flavus (MTTC-3396) which exhibits a broad spectrum of in vitro antibacterial activity against human infecting bacterial pathogens and the high bioactive metabolites production. The effect of various parameters viz., incubation time, temperature, pH, carbon and nitrogen sources, and sodium chloride concentration on antibacterial metabolite production were studied by varying single parameter at a time. It was found that metabolite production by this isolate was greatly influenced by various cultural conditions. The optimum carbon source starch, nitrogen source beef extract, pH 6.0, temperature at 40oC, 3 % NaCl concentration and incubation period of 144 h were found for the maximum metabolite production.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2016-10-31

Issue

Volume 2, Issue 5, September-October-2016

Section

Research Articles

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How to Cite

[1]
Kalyani. P, Geetha. S, Hemalatha. K. P. J, " In Vitro, Optimization of Antibacterial Activity of Secondary Metabolites Produced By Aspergillus Flavus (Mtcc-3396), International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 2, Issue 5, pp.487-491, September-October-2016.