Partial Loss of Cytochrome C by Mitochondria of Pea Seedlings Under Conditions of Water Scarcity

Authors

  • Irina Zhigacheva  Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow
  • Vladimir Biryukov  Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow
  • Elena Mil  Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow

Keywords:

Water Deficiency, Mitochondria, Cardiolipin, Cytochrome C

Abstract

We investigated the effect of water deficiency and treatment of pea seedlings with 10-11M solution of a synthetic phenolic antioxidant - potassium phenosan on the functional state of mitochondria of pea seedlings. Water deficiency, triggering lipid peroxidation, leads to a decrease in the content of unsaturated fatty acids containing 18 and 20 carbon atoms in the mitochondrial membranes, decreases the maximum oxidation rates of NAD-dependent substrates and succinate also the electron transport rates at the terminal stage ( the cytochrome oxidase stage) of the mitochondrial respiratory chain. In addition, water deficiency causes mitochondrial swelling. Introduction into the incubation medium of mitochondria 5×10-6M of cytochrome C or 10-11M of potassium phenosan restores the electron transfer rates at this stage of the respiratory chain. The antioxidant prevents mitochondrial dysfunction. It is assumed that a breach of the bioenergetics characteristics of mitochondria in conditions of water deficiency apparently connected with the oxidation of unsaturated fatty acids mainly the linoleic acid, which are included in the composition of the cardiolipin .Potassium phenosan, preventing lipid peroxidation, restores the functional activity of mitochondria..

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2016-10-30

Issue

Volume 2, Issue 5, September-October-2016

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Research Articles

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How to Cite

[1]
Irina Zhigacheva, Vladimir Biryukov, Elena Mil, " Partial Loss of Cytochrome C by Mitochondria of Pea Seedlings Under Conditions of Water Scarcity , International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 2, Issue 5, pp.369-376, September-October-2016.