Structure-Function Analysis of  Toxin-like Protein of Haliclona molitba  Associated with Symbiotic Bacteria from Indonesian Islands

Authors(3) :-Vivitri D. Prasasty, Ritchie Rahardja, Rory A. Hutagalung

Tropical marine sponge, Haliclona molitba is collected from Indonesian archipelago and investigated for toxin-like protein activities. The aim of this research is to determine the structure-function relationships of the toxin-like protein, especially for hemolytic and hem agglutination activities which have wide range potentials as anticancer, antitumor, and antimicrobial, from two distinguished type of sponges: cultured (A03k) and uncultured (A03) Haliclona molitba. The crude toxin-like proteins were extracted from A03k and A03 at concentration 0.26 mg/mL and 0.23 mg/mL in aqueous extracts, respectively. On SDS-PAGE, the crude protein yielded five well-defined bands around 17, 24, 36, 96, and 120 kDa from A03 and four well-defined bands around 17, 37, 44 and 96 kDa from A03k. Hemolytic and hemagglunation assays were conducted by utilizing 8 weeks old of male Sprague Dawley rat erythrocyte. The results showed that hemolytic activity was found on protein extracted from A03k while hemagglutinating activities were not found in both cultured (A03k) and unculterd (A03) of Haliclona molitba.  Symbiotic bacteria associated with Haliclona molitba are predicted as Enterobacter sp. This finding revealed that the marine sponge is a potential source of novel bioactive proteins.

Authors and Affiliations

Vivitri D. Prasasty
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Indonesia
Ritchie Rahardja
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Indonesia
Rory A. Hutagalung
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Indonesia

Toxin-Like Protein, Haliclona Molitba, Hemolysis, Hemagglutination, Symbiotic Bacteria

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Publication Details

Published in : Volume 3 | Issue 1 | January-February 2017
Date of Publication : 2017-02-28
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 01-05
Manuscript Number : IJSRSET1626156
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Vivitri D. Prasasty, Ritchie Rahardja, Rory A. Hutagalung, " Structure-Function Analysis of  Toxin-like Protein of Haliclona molitba  Associated with Symbiotic Bacteria from Indonesian Islands, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 1, pp.01-05, January-February-2017.
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