Structure-Function Analysis of  Toxin-like Protein of Haliclona molitba  Associated with Symbiotic Bacteria from Indonesian Islands

Authors

  • Vivitri D. Prasasty  Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Indonesia
  • Ritchie Rahardja  Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Indonesia
  • Rory A. Hutagalung  Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Indonesia

Keywords:

Toxin-Like Protein, Haliclona Molitba, Hemolysis, Hemagglutination, Symbiotic Bacteria

Abstract

Tropical marine sponge, Haliclona molitba is collected from Indonesian archipelago and investigated for toxin-like protein activities. The aim of this research is to determine the structure-function relationships of the toxin-like protein, especially for hemolytic and hem agglutination activities which have wide range potentials as anticancer, antitumor, and antimicrobial, from two distinguished type of sponges: cultured (A03k) and uncultured (A03) Haliclona molitba. The crude toxin-like proteins were extracted from A03k and A03 at concentration 0.26 mg/mL and 0.23 mg/mL in aqueous extracts, respectively. On SDS-PAGE, the crude protein yielded five well-defined bands around 17, 24, 36, 96, and 120 kDa from A03 and four well-defined bands around 17, 37, 44 and 96 kDa from A03k. Hemolytic and hemagglunation assays were conducted by utilizing 8 weeks old of male Sprague Dawley rat erythrocyte. The results showed that hemolytic activity was found on protein extracted from A03k while hemagglutinating activities were not found in both cultured (A03k) and unculterd (A03) of Haliclona molitba.  Symbiotic bacteria associated with Haliclona molitba are predicted as Enterobacter sp. This finding revealed that the marine sponge is a potential source of novel bioactive proteins.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-02-28

Issue

Volume 3, Issue 1, January-February-2017

Section

Research Articles

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How to Cite

[1]
Vivitri D. Prasasty, Ritchie Rahardja, Rory A. Hutagalung, " Structure-Function Analysis of  Toxin-like Protein of Haliclona molitba  Associated with Symbiotic Bacteria from Indonesian Islands, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 1, pp.01-05, January-February-2017.