Screening and Production of Lipase Producing Bacteria from Vegetable Oil Industry

Authors(3) :-Veerapagu. M, Jeya K. R., Sankaranarayanan. A

Lipases are industrially important enzymes having applications in numerous industries. The present study aims to isolate different bacteria and screen them for lipase production. Oil spilled soil samples were collected from vegetable oil industry of Harur, Dharmapuri district. A total of thirteen morphologically distinct bacteria isolated were screened for lipase production in Tween 80 agar plate. Lipolytic activity was confirmed by zone of precipitation around the colony. Among thirteen isolates, six isolates exhibited lipolytic activity in plate assay. The bacterial isolate HLB7 produced high amount of lipase 2.6U/ml in lipase production medium by submerged fermentation was identified as Pseudomonas sp. The optimum incubation time, inoculum size, temperature and pH for lipase production were determined. The lipase production was maximum at incubation time 48 hr (3.26U/ml), Inoculum size 4% (4.37U/ml), temperature 40?C (4.65U/ml) and pH 7 ( 4.95U/ml) by the Pseudomonas sp

Authors and Affiliations

Veerapagu. M
Department of Biotechnology, P. G. Extension Centre, Bharathidasan University, Kurumbalur, Perambalur, Tamil Nadu, India
Jeya K. R.
Department of Biotechnology, Bharathidasan University Constituent Model College (W), Veppur, Perambalur, Tamil Nadu, India
Sankaranarayanan. A
C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Tarsadi, Surat, Gujarat

Oil Spilled Soil, Lipase, Fermentation, Pseudomonas, Tween80 Agar

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Publication Details

Published in : Volume 2 | Issue 6 | November-December 2016
Date of Publication : 2016-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 726-732
Manuscript Number : IJSRSET1626172
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Veerapagu. M, Jeya K. R., Sankaranarayanan. A, " Screening and Production of Lipase Producing Bacteria from Vegetable Oil Industry, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 2, Issue 6, pp.726-732, November-December-2016.
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