Screening and Production of Lipase Producing Bacteria from Vegetable Oil Industry

Authors

  • Veerapagu. M  Department of Biotechnology, P. G. Extension Centre, Bharathidasan University, Kurumbalur, Perambalur, Tamil Nadu, India
  • Jeya K. R.  Department of Biotechnology, Bharathidasan University Constituent Model College (W), Veppur, Perambalur, Tamil Nadu, India
  • Sankaranarayanan. A  C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Tarsadi, Surat, Gujarat

Keywords:

Oil Spilled Soil, Lipase, Fermentation, Pseudomonas, Tween80 Agar

Abstract

Lipases are industrially important enzymes having applications in numerous industries. The present study aims to isolate different bacteria and screen them for lipase production. Oil spilled soil samples were collected from vegetable oil industry of Harur, Dharmapuri district. A total of thirteen morphologically distinct bacteria isolated were screened for lipase production in Tween 80 agar plate. Lipolytic activity was confirmed by zone of precipitation around the colony. Among thirteen isolates, six isolates exhibited lipolytic activity in plate assay. The bacterial isolate HLB7 produced high amount of lipase 2.6U/ml in lipase production medium by submerged fermentation was identified as Pseudomonas sp. The optimum incubation time, inoculum size, temperature and pH for lipase production were determined. The lipase production was maximum at incubation time 48 hr (3.26U/ml), Inoculum size 4% (4.37U/ml), temperature 40?C (4.65U/ml) and pH 7 ( 4.95U/ml) by the Pseudomonas sp

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2016-12-31

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Volume 2, Issue 6, November-December-2016

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Research Articles

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[1]
Veerapagu. M, Jeya K. R., Sankaranarayanan. A, " Screening and Production of Lipase Producing Bacteria from Vegetable Oil Industry, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 2, Issue 6, pp.726-732, November-December-2016.