Isolation of Antibiotic Producing Actinomycetes from Untapped Soils of Yarada Hills And Assessment of their Antimicrobial Activities

Authors

  • K. Sunanda Kumari  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India
  • P. Kalyani  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India
  • K. Chandana Vineela  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India
  • V. Hemalatha  Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India
  • K. P. J. Hemalatha  Advanced Analytical laboratory, Andhra University, Visakhapatnam, Andhra Pradesh, India

Keywords:

Actinomycetes, Antibiotics, Streptomyces, Submerged Fermentation.

Abstract

The purpose of this study was to isolate the antibiotic producing actinomycetes from soil from different parts of Yarada hills and to access their antimicrobial activity against different pathogenic bacteria like Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). This study also focuses on investigating the distribution of the antibiotic producing actinomycetes according to the texture and cultivation status of soil. Seventy four colonies of actinomycetes were isolated from the soil collected from five different parts of Yarad hills in the standard medium out of which 10 colonies produced antibiotics; all of them belonged to genus Streptomyces. One of the isolated colony largely inhibited the growth of E. coli and Staphylococcus aureus with zone of inhibition more than 20 mm for both. Antibiotic from this colony was produced by submerged fermentation and the minimum inhibitory concentration of the crude antibiotic was determined.

References

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-04-30

Issue

Volume 3, Issue 2, March-April-2017

Section

Research Articles

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How to Cite

[1]
K. Sunanda Kumari, P. Kalyani, K. Chandana Vineela, V. Hemalatha, K. P. J. Hemalatha, " Isolation of Antibiotic Producing Actinomycetes from Untapped Soils of Yarada Hills And Assessment of their Antimicrobial Activities, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 2, pp.290-293, March-April-2017.