Isolation, Screening and Production of Bacterial Protease Enzyme

Authors(2) :-Rajee T, Selvamaleeswaran P

The objective of present study was to isolate and screen proteolytic bacteria from poultry waste site at Rasipuram, Namakkal district, Tamilnadu, India. Soil samples were serially diluted and 0.1ml of sample was spreaded on nutrient agar plate at 37o C for 48 hrs. Four bacterial colonies isolated were screened for proteolytic activity by skim milk agar plate assay. The isolate RPB 2 showed maximum zone of hydrolysis (13mm) was selected and identified as Staphylococcus sp by morphological and biochemical test. Various factors influencing the protease production such as incubation time, initial pH, incubation temperature, inoculum level and casein concentration were studied. The results showed that maximum protease enzyme production occurred at incubation time 48hr (34 U/ml), pH 7.0 (42U/ml), 2% inoculum concentration (21.5U/ml), temperature 37o C (51U /ml) and 2% casein concentration (72U/ml).

Authors and Affiliations

Rajee T
Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram,Tamil Nadu, India
Selvamaleeswaran P
Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram,Tamil Nadu, India

Protease, Poultry Waste Soil, Skim Milk Agar, Zone Of Hydrolysis, Fermentation, Staphylococcus.

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Publication Details

Published in : Volume 3 | Issue 3 | May-June 2017
Date of Publication : 2017-06-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 447-452
Manuscript Number : IJSRSET1733113
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Rajee T, Selvamaleeswaran P, " Isolation, Screening and Production of Bacterial Protease Enzyme, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 3, pp.447-452, May-June-2017.
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