Factors Effect on Antifungal Activity of Lactic Acid Bacteria against Fusarium Proliferatum Isolate from Rose Leaves

Authors

  • Akaram Husain  Food Biotechnology Department, Faculty of Science and Technology, University Sains Islam Malaysia, Bandar Baru Nilai, Negeri Sembilan, Malaysia
  • Zaiton Hassan  Food Biotechnology Department, Faculty of Science and Technology, University Sains Islam Malaysia, Bandar Baru Nilai, Negeri Sembilan, Malaysia
  • Mohd Nizam Lani  School of Food Science and Technology, University Malaysia Terengganu, Kuala Terengganu, Malaysia

Keywords:

Fusarium proliferatum-LR, Lactic Acid Bacteria, Antifungal Activity.

Abstract

Microorganism’s lactic acid bacteria (LAB) are known to have antifungal activity, but its utilisation, as protection agent against fungi in plant is limited. The present study aimed at evaluating lactic acid bacteria (LAB) as bio-control agent against fungi F. proliferatum isolated from rose leaves. Five LAB strains namely, Lactobacillus plantarum 1MSS and Pediococcus pentosaceus 1MSS, Lactobacillus plantarum 1FF, Lactobacillus acidophilus ATCC 314 and Lactobacillus plantarum ATCC 8014 were evaluated for antifungal activity against the F. proliferatum-LR by several methods. Cells free supernatants (LAB-CFS) of all isolates showed strong antifungal activity (3.26 mm to 3.76 mm) evaluated by well diffusion method (43.00 mm to 49.66 mm) within 48 h at 28oC.  LAB-CFS reduced fungi hyphal growth on potato dextrose agar (PDA) and in malt extracts broth (MEB) from 32.9% to 43.8% and 93.79% to 94.65%, respectively. Enzyme treatments using pepsin, papain and proteinase K reduced the antifungal activity from 63.85% to 87.21% depending on enzyme used and LAB-CFS.  Adjusting pH of LAB-CFS (pH 2 to 5) did not diminish the antifungal activity of Lb. plantarum 1MSS and P. pentosaceus 1MSS. The antifungal activity of cell supernatant of LAB was maintained after subjected to 80oC and 90oC and 121oC for 30 min. Growth of F. proliferatum was inhibited by both cells and supernatants of the LAB. The LAB-CFS contains heat stable compounds that effective to be used as bio-control against F. proliferatum-LR that commonly infect rose plants.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-08-31

Issue

Volume 3, Issue 5, July-August-2017

Section

Research Articles

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How to Cite

[1]
Akaram Husain, Zaiton Hassan, Mohd Nizam Lani, " Factors Effect on Antifungal Activity of Lactic Acid Bacteria against Fusarium Proliferatum Isolate from Rose Leaves, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 5, pp.449-455, July-August-2017.