Molecular Detection of Extended Spectrum Beta Lactamase TEM Gene Produced Encoding Klebsiella Species in Khartoum State Sudan 2017

Authors

  • Nada Makeen Eldeen Mohamed Ahmed  MSc Student, Faculty of Medical Laboratory Sciences, AL Neelain University, Sudan
  • Mohamed Hussein Arbab  Assistant Professor, Omdurman Ahlia University, Microbiology Department, Sudan
  • Sahar Mohamed Seed Ahamed  Lectuere, Omdurman Ahlia University ,Microbiology Department, Sudan

DOI:

https://doi.org//10.32628/18410IJSRSET

Keywords:

ESBLs,Cephalosporin, Enterobacteriaceae, TEM Gene.

Abstract

Background: Resistance to variety of common antimicrobials has made the proliferation of extended spectrum β-lactmase (ESBL) producing strains a serious global health concern that complicated treatment strategies. Antimicrobial resistance to cephalosporin, penicillin and aztreonam are bmediated by Extended-Spectrum Beta-Lactamases (ESBL) via hydrolysis of antibiotics. The most common bacteria associated with ESBL among the Enterobacteriaceae are Klebsiella species. This study was focus on detection of TEM gene associated ESBL Klebsiella species in Khartoum stat.

Methods : A total of 100 ESBL klebsiella species were isolated, strains confirmation was take place using API detection protocol, and ESBL phenotype was determined by a double disk diffusion test (DDDT) and E – test, the qualified strains were examined for the existence of TEM-1 gene using convential PCR.

Results : Out of 100 ESBL Klebsiella species 20 (20%) were positive for TEM gene. A frequent of 18 out of 20 strains isolated was causing UTI and most of the samples were collected from females.

Conclusion : Study was revealed that the prevalence of TEM gen among TEM gene associated ESBL Klebsiella species in Khartoum stat represent 20%, which is require farther phenotypic analysis to sub genotyping the TEM gene.

References

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  1. Lai CC, Lee K, Xiao Y, Ahmad N, Veeraraghavan B, Thamlikitkul V, et al. High burden of antimicrobial drug resistance in Asia. Journal of Global Antimicrobial Resistance. 2014; 2: 141-147. http://dx.doi.org/10.1016/j.jgar.2014.02.007
  2. Li B, Yi Y, Wang Q, Woo PC, Tan L, Jing H, et al. Analysis of drug resistance
  3. determinants in Klebsiella pneumoniae isolates from a tertiary-care hospital in Beijing,
  4. China. PLoS One. 2012; 7: e42280. doi: 10.1371/journal.pone.0042280. pmid:2286010
  5. African Journal of Microbiology Research Vol. 4 (8), pp. 650-654, 18 April, 2010
  6. Available online http://www.academicjournals.org/ajmr ISSN 1996-0808
  7. Y. Harada, Y. Morinaga, K. Yamada et al., “Clinical and molecular epidemiology of extended-spectrum -lactamaseproducing Klebsiella pneumoniae and Escherichia coli in a Japanese Tertiary Hospita,” Journal of Medical Microbiology &Diagnosis, vol. 2, no. 3, pp. 2161-2170, 2013.
  8. J. Du, P. Li, H. Liu, D. L¨u, H. Liang, and Y. Dou, “Phenotypic andmolecular characterization ofmultidrug resistant Klebsiella pneumoniae isolated from a University Teaching Hospital, China,” PLoS ONE, vol. 9, no. 4,Article ID e95181, 2014.
  9. H. Amraie,P. Shakib, S. Rouhi, N. Bakhshandeh, andB.Zamanzad, “Prevalence assessment of magA gene and antimicrobial susceptibility of Klebsiella pneumoniae isolated from clinical specimens in Shahrekord, Iran,” Iranian Journal ofMicrobiology, vol. 6, no. 5, pp. 311-316, 2014.
  10. Extended-spectrum beta-lactamase genes of Klebsiella pneumoniae strains in Taiwan: recharacterization of shv-27, shv-41, and tem-11 .6Lin TL, et al .Microb Drug Resist.2006.
  11. Rice L.B., Willey S.H., Papanicolaou G.A., Medeiros A.A., Eliopoulos G.M., Moellering R.C.J., Jacoby G.A. Outbreak of ceftazidime resistance caused by extended- Spectrum beta-lactamases at a Massachusetts chronic-care facility. Antimicrob. Agents Chemother. 1990;34(11):2193-2199.
  12. Du Bois S.K., Marriott M.S., Amyes S.G. TEM- and SHV-derived extended- spectrum beta-lactamases: relationship between selection, structure and function. J. Antimicrob. Chemother. 1995;35:7-22
  13. Clinical Laboratory Standards Institute. Performance standards for antimicrobial disc susceptibility tests, IXX international Supplement (M100- S19). Wayne Pa, USA: National Committee for Clinical Laboratory Standards. 2009.
  14. Khorshidi A, Rohani M, Moniri R. The prevalence and molecular characterization of extended-spectrum β-lactamases-producing Klebsiella pneumoniae isolates recovered from Kashan hospital university, Iran. Jundishapur J Microbiol. 2012;4(4):289-94.
  15. Mendes C, Kiffer C, Segura A, Ribeiro J, Turner P. Klebsiella pneumoniae with multiple antimicrobial resistance. Braz J Infect Dis. 2004;8(1):109-11.
  16. Putman M, van Veen HW, Konings WN. Molecular properties of bacterial multidrug transporters. Microbiol Mol Biol Rev. 2000;64(4):672-93
  17. Karimi A, Rahbar M, Fallah F, Navidinia M, Malekan M. Detection of integron elements and gene groups encoding ESBLs and their prevalence in Escherichia coli and Klebsiella isolated from urine samples by PCR method. Afr J Microbiol Res. 2012;6(8):1806-9.16. Branger C, Lesimple AL, Bruneau B, Berry P, Lambert-Zechovsky
  18. Branger C, Lesimple AL, Bruneau B, Berry P, Lambert-Zechovsky N. Long-term investigation of the clonal dissemination of Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases in a university hospital. J Med Microbiol. 1998; 47(3):201-9
  19. Wang XR, Chen JC, Kang Y, Jiang N, An SC, Gao ZC. Prevalence and characterization of plasmid-mediated blaESBL with their genetic environment in Escherichia coli and Klebsiella pneumoniae in patients with pneumonia. Chin Med J (Engl). 2012;125(5):894-900
  20. Feizabadi MM, Mahamadi-Yeganeh S, Mirsalehian A, Mirafshar SM, Mahboobi M, Nili F, et al. Genetic characterization of ESBL producing strains of Klebsiella pneumoniae from Tehran hospitals. J Infect Dev Ctries. 2010;4(10):609-15.
  21. M.-F. Lartigue, N. Fortineau, P. Nordmann. Spread of novel expanded-spectrum β-lactamases in Enterobacteriaceae in a university hospital in the Paris area, France. 22 May 2005. 10.1111/j.1469-0691.2005.01172
  22. De Champs C, Chanal C, Sirot D et al. Frequency and diversity of Class A extended-spectrum β-lactamases in hospitals of the Auvergne, France: a 2 year prospective study. Antimicrob Cemother.

International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-10-30

Issue

Volume 4, Issue 10, September-October-2018

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Research Articles

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How to Cite

[1]
Nada Makeen Eldeen Mohamed Ahmed, Mohamed Hussein Arbab, Sahar Mohamed Seed Ahamed, " Molecular Detection of Extended Spectrum Beta Lactamase TEM Gene Produced Encoding Klebsiella Species in Khartoum State Sudan 2017, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 10, pp.360-366, September-October-2018. Available at doi : https://doi.org/10.32628/18410IJSRSET