In vitro Studies on the Effect of Ethanol Extract of Trachyspermum Ammi on Lactate Dehydrogenase of Haemonchus Contortus

Authors(2) :-S. Sathish Kumar, Dr. L. Veerakumari

Gastro intestinal helminth infections is a major threat to small ruminant production. Haemonchous contortus is a nematode that feeds on blood of small ruminants and causes anemia, anorexia and eventually leads to death. Anthelmintic drugs are used to treat helminth infection for control of the parasitic diseases. The problem of the development of resistance against the common chemical anthelmintics has renewed the interest in the study of medicinal plants for the development of herbal anthelmintics. Lactate Dehydrogenase (LDH) a glycolytic enzyme plays a key role in the carbohydrate metabolism of the parasites. It catalyzes the reduction of pyruvate to lactate and oxidation of lactate to pyruvate. In the present investigation anthelmintic efficacy of ethanol extract of Trachyspermum ammi (TaEE) on LDH of the nematode H. contortus was studied in vitro.  The worms were exposed to five different sub-lethal concentrations (0.01, 0.05, 0.1, 0.5 and 1%) of ethanol extract of T. ammi (TaEE), which significantly inhibited the LDH activity catalyzing both oxidation of lactate and reduction of pyruvate. Inhibition of LDH activity increased with an increase in the drug concentration and the period of exposure. Hindrance in LDH activity reduce the level of ATP production which affects the physiological functions of the parasite and ultimately leads to death of the worms.

Authors and Affiliations

S. Sathish Kumar
Ph.D Research scholar, PG and Research, Department of Zoology, Pachaiyappa’s College, Chennai, Tamil Nadu, India
Dr. L. Veerakumari
Associate Professor and Head (Retd.), PG and Research Department of Zoology, Pachaiyappa’s College, Chennai, Tamil Nadu, India

Haemonchous Contortus, Trachyspermum Ammi, Lactate Dehydrogenase.

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Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 750-753
Manuscript Number : IJSRSET1738206
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

S. Sathish Kumar, Dr. L. Veerakumari, " In vitro Studies on the Effect of Ethanol Extract of Trachyspermum Ammi on Lactate Dehydrogenase of Haemonchus Contortus , International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.750-753, November-December-2017.
Journal URL : http://ijsrset.com/IJSRSET1738206

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