Anthelmintic Efficacy of Ethanol Extract of Albizia lebbeck and Trachyspermum ammi on the Glutathione-s-transferase of Cotylophoron cotylophorum

Authors(3) :-C. Manigandan, Dr. N. Shettu, Dr. L. Veerakumari

Sheep is an economically important livestock species, mostly reared for meat and wool production. Infection with gastrointestinal helminths has been identified as one of the causes for the production loss in sheep rearing, which arise primarily through severe weight loss, poor meat, milk and wool production, impaired reproductive performance, mortality, carcass and offal condemnation. Paramphistomosis caused by amphistomes constitutes a major group of diseases, causing considerable economic loss to livestock industry in India. Chemical control of helminths coupled with improved management has important parasitic control strategy throughout the world. However increasing problems of development of resistance in helminths against anthelmintics led to the proposal of screening medicinal plants for their anthelmintic activity. Glutathione-s-transferase (GST) is multifunctional enzyme that participates in the detoxification of endogenous and exogenous toxic metabolites which is fatal to the parasites. In the present investigation, effect of ethanol extracts of Albizia lebbeck (AlEE) and Trachyspermum ammi (TaEE) on GST activity against the paramphistome Cotylophoron cotylophorum was studied in vitro. The parasites were exposed to five sub-lethal concentrations of AlEE and TaEE for 2, 4 and 8h. Maximum inhibition of GST activity was observed in 0.5 mg/ml concentration after 8h of exposure. Inhibition of GST activity was dose and time dependent. Decrease in GST lead to accumulation of toxic metabolites which is lethal to the parasites.

Authors and Affiliations

C. Manigandan
Ph.D., Research scholar, PG and Research, Department of Zoology, Pachaiyappa’s college, Chennai, Tamil Nadu,India
Dr. N. Shettu
Associate Professor and Head, PG and Research Department of Zoology, Pachaiyappa’s college, Chennai, TamilNadu, India
Dr. L. Veerakumari
Associate Professor and Head (retd), PG and Research Department of Zoology, Pachaiyappa’s college, Chennai,Tamil Nadu, India

Cotylophoron cotylophorum, Albizia lebbeck, Trachyspermum ammi, Glutathione-s-transferase.

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Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 754-758
Manuscript Number : IJSRSET1738207
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

C. Manigandan, Dr. N. Shettu, Dr. L. Veerakumari, " Anthelmintic Efficacy of Ethanol Extract of Albizia lebbeck and Trachyspermum ammi on the Glutathione-s-transferase of Cotylophoron cotylophorum, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.754-758, November-December-2017.
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