Anthelmintic Efficacy of Ethanol Extract of Albizia lebbeck and Trachyspermum ammi on the Glutathione-s-transferase of Cotylophoron cotylophorum

Authors(3) :-C. Manigandan, Dr. N. Shettu, Dr. L. Veerakumari

Sheep is an economically important livestock species, mostly reared for meat and wool production. Infection with gastrointestinal helminths has been identified as one of the causes for the production loss in sheep rearing, which arise primarily through severe weight loss, poor meat, milk and wool production, impaired reproductive performance, mortality, carcass and offal condemnation. Paramphistomosis caused by amphistomes constitutes a major group of diseases, causing considerable economic loss to livestock industry in India. Chemical control of helminths coupled with improved management has important parasitic control strategy throughout the world. However increasing problems of development of resistance in helminths against anthelmintics led to the proposal of screening medicinal plants for their anthelmintic activity. Glutathione-s-transferase (GST) is multifunctional enzyme that participates in the detoxification of endogenous and exogenous toxic metabolites which is fatal to the parasites. In the present investigation, effect of ethanol extracts of Albizia lebbeck (AlEE) and Trachyspermum ammi (TaEE) on GST activity against the paramphistome Cotylophoron cotylophorum was studied in vitro. The parasites were exposed to five sub-lethal concentrations of AlEE and TaEE for 2, 4 and 8h. Maximum inhibition of GST activity was observed in 0.5 mg/ml concentration after 8h of exposure. Inhibition of GST activity was dose and time dependent. Decrease in GST lead to accumulation of toxic metabolites which is lethal to the parasites.

Authors and Affiliations

C. Manigandan
Ph.D., Research scholar, PG and Research, Department of Zoology, Pachaiyappa’s college, Chennai, Tamil Nadu,India
Dr. N. Shettu
Associate Professor and Head, PG and Research Department of Zoology, Pachaiyappa’s college, Chennai, TamilNadu, India
Dr. L. Veerakumari
Associate Professor and Head (retd), PG and Research Department of Zoology, Pachaiyappa’s college, Chennai,Tamil Nadu, India

Cotylophoron cotylophorum, Albizia lebbeck, Trachyspermum ammi, Glutathione-s-transferase.

  1. Anand, L., Dhanachand, C., Mohilal, N. and Sharatkumar, S., 2000. Preva-lence of intestinal helminths in dairy cattle of Manipur, Uttar Pradesh J. Zoo., 20: 93-95.
  2. Brophy, P. M. and Pritchard, D. I., 1994. Parasitic helminth glutathione-Stransferases: an update on their potential as targets for immuno and chemotherapy. Exptl. Parasitol., 79: 89-96.
  3. Chulet Rahul, Joseph Lincy, Mathew George and Pradhan pankaj, 2010. Pharmacognostic standardization and phytochemical sscreening of Albizia lebbeck. J. chem, pharm. Res., 2(1): pp.432-443.
  4. Coles, B. F. and Kadlubar, F. F., 2003. Detoxification of electrophilic compounds by glutathione-S-transferase catalysis: Determinants of individual response to chemical carcinogens and chemotherapeutic drugs. BioFactors., 17(1-4):115-130.
  5. Farahnak, A., Anvar-Seyyedi, M., Jalali, M. and Esharghian, M. R., 2006. Study on Glutathione-S-Transferase inhibition assay by triclabendazole on Fasciola spp. Acta. Medica. Iranica., 44 (6): 371-376.
  6. Ganguli, N. B. and Bhatt, R.M., 1993. Mode of action of active principles from stem bark of Albizia lebbeck. Ind. J. Exp. Biol., 31: pp.125-129.
  7. Gupta, S. and Rathaur, S., 2005. Filarial Glutathione-S-Transferase: its induction by xenobiotics and potential as drug target. Acta. Biochim. Pol., 52 (2): 493-500.
  8. Habig, W. H., Pabst, M. J. and Jakoby, W. B., 1974. Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249: 7130-7139.
  9. Harborne, J. B., 1998. Phytochemical Methods A guide to modern techniques of plant analysis. 3rd Edition, published by Chapman & Hall, London, pp. 302.
  10. Hein, W. R. and Harrison, G. B. L., 2005. Vaccines against veterinary helminths. Vet. Parasitol., 132: 217-222.
  11. Joshi, S. G., 2000. Medicinal Plants, Ist ed. Delhi (INDIA): Oxford and IBH Publisher.
  12. Kamal Jeet1, Nisha Devi, Thakur Narender, Tomar Sunil, Shalta Lalit and Thakur Raneev, 2012. Trachyspermum ammi (ajwain): a comprehensive review. Int. Res. J. Pharm., 3(5). Pp. 133-138.
  13. Klassen, C.D., 1996. Casarett and dowll’s Toxicology. The basic science of poisons, 5th edition M.C. Grawhill New York.
  14. Krishnamoorthy, V. and Madalageri, M. B., 1999. Bishop weeds (Trachyspermum ammi): an essential crop for north Karnatka. J. Medi. and Aromatic Plant Sci., 1999; 21(4): Pp. 996-998.
  15. Little, J., Sharp, L. and Masson, L. F., 2006. Colorectal cancer and genetic polymorphisms of CYPIAL, GSTM1 and GSTT1: a case-control study in the Grampian region of Scotland. Int. J. cancer, 119: 2155-64.
  16. Manoj Dhanraj, K. and Veerakumari, L., 2015. Effect of ethanol extract of Areca catechu and Syzygium aromaticum on GST of Cotylophoron cotylophorum. World J. of Pharma. and Parmaceut. Sci., 4 (10): 1117-1125.
  17. Millar, M., Colloff, A. and Scholes, S., 2012. Disease associated with imma-ture paramphistome infection, Vet. Record., 171: 509-510.
  18. Mohammed Faisal, Singh, P. P. and Irchhaiya, R., 2012. Review of Albizia lebbeck a potent herbal drug. Int. Res. J. Pharmacy., 3(5): pp. 63-68.
  19. Mohammed Farag, Ali El Gamal, Ashraf Kalil, Adnan Al- Rehaily, Omar El Mirghany and Kamal El Tahil. 2013. Evaluationof same biological activites of Albizia lebbeck flowers. Pharmacol. and Pharm., 4: 473-477.
  20. Rao, U. R., Salinas, G., Mehta, K. and Klei, T. R., 2000. Identification and localization of glutathione-S-transferase as a potential target enzyme in Brugia species. Parasitol. Res., 86: 908-915.
  21. Rashid, R. B., Chowdhury, R., Jabbar, A., Hasan, C. M. and Rashid, M. A., 2003. Constituents of Albizia lebbeck and antibacterial activity of an isolated flavone derivative. Saudi. Pharm. J., 11(1-2): 52-6.
  22. Resmi, C. R., Venukumar, M. R. and Latha, M. S., 2006. Anti-oxidant activity of Albizia lebbeck (Linn.) Bent. In Al-loxan diabetic rats. Ind. J. of Phy. Pharmcol., Vol. 50(3): pp. 297-302.
  23. Sadiq, U., Asif, M., Sajad Mir, Meraj, A. Md., Hussain, U. and Wasim, A., 2012. Anti- inflammatory and antioxidant activity of Trachyspermum ammi seeds in collagen induced arthritis in rats. Int. J. Drug Dev. & Res., 4 (1): 210-219.
  24. Shamina, A., Rosana, O. B, Shaji, A., Riju, A., Reena, N. and Eapen, S. J., 2010. http://220.227.138.213/gstleadbase/index.php?centre=overview.php.
  25. Singh, I. and Singh, V. P., 2000. Antifungal properties of aqueous and organic extracts of seed plants against Aspergillus flavus and A. niger. Phytomorphol., 20: 151-157.
  26. Singh, S. and Irshadullah, M., 2003. Kinetic studies and in vitro effect of anthelmintics on glutathione S-transferase of Fasciola gigantica. J. Vet. Parasitol., 17 (1): 27-31.
  27. Singh, Y. N., Bisht, H. and Panday, 1991. “Effect of dry seed extract of a medicinal plant Albizia lebbeck on Testicular and Epididymal protein profiles of rat”. Him. J. of Environ. And Zoo., Vol. 5(1): pp.94-108.
  28. Sivropoulou, A., Papanikolaou, E., Nilolaou, C., Kokkini, S., Lanaras, T. and Arsenakis, M., 1996. Antimicrobial and cytotoxic activities of origanum essential oils. J. Agric. Food Cheml., 44: 1202-1205.
  29. Soulsby, E. J. L., 2006. Helminths, arthropods and protozoa of domesticated animals, 7th Ed. Bailliere Tindall, London., pp. 69-71.
  30. Srivastava, K. C., 1988. Extract of a spice Omum (Trachyspermum ammi) shows antiaggregatory effects and alters arachidonic acid metabolism in human platelets. Prostaglandins Leukot Essent Fatty Acids. 33: 1-6.
  31. Vasanthi padanabhan, Manimekalai, Ganapathy and Vasanthi Kennedy Evanjelene, 2013. Preliminary phytochemical and Anti-bacterial studies on flowers and pods of Albizia lebbeck (Benth). Int. J. Emerging Tech. and Advanced Engineering, Vol. 3(9): pp.541-544.
  32. Veerakumari, L., 1996. In vitro studies on the effect of some anthelmintics on Cotylophoron cotylophorum (Fischoeder, 1901) (Digenea: Paramphistomidae). A structural and biochemical analysis. Ph.D. thesis submitted to the University of Madras, Chennai.
  33. Wolstenholme, A. J., Fairweather, I., Prichard, R., Von Samson-Himmelstjerna, G. and Sangster, N. C., 2004. Drug resistance in veterinary helminths. Trends Parasitol., 20: Pp.469-76.

Publication Details

Published in : Volume 3 | Issue 8 | November-December 2017
Date of Publication : 2017-12-31
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 754-758
Manuscript Number : IJSRSET1738207
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

C. Manigandan, Dr. N. Shettu, Dr. L. Veerakumari, " Anthelmintic Efficacy of Ethanol Extract of Albizia lebbeck and Trachyspermum ammi on the Glutathione-s-transferase of Cotylophoron cotylophorum, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.754-758, November-December-2017. Citation Detection and Elimination     |     
Journal URL : https://ijsrset.com/IJSRSET1738207

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