Prevalence and Molecular Detection of West Nile Virus (WNV) Among Renal Transplant Patients in Khartoum State, Sudan

Authors(4) :-Alia H Abaallh, Abdel Rahim M El Hussein, Isam M Elkhidir, Khalid A Enan

Background:
West Nile virus (WNV) is an arbovirus from Flaviviridae family .West Nile now represents one of the most common arboviral diseases worldwide that causes febrile illness.Also, significant number of patients develop severe neurological disease including meningitis, encephalitis, and acute paralysis. This study was carried out to detect the frequency of West Nile virus IgM antibodies and virus nucleic acid among renal transplant patients in Khartoum state.
Method:
This was a descriptive  study, .in which serum specimens were collected from 93patient (68male,25female) and investigated for WNV specific immunoglobulin M( IgM) using enzyme-linked immunosorbant assay(ELISA) and for WNV RNA using real time PCR (RT-PCR)   The study group age ranged from 20 to 80years
Result:
Out of the the 93 patients tested, 7(7.5%) were positive for IgM and 86 (92.4%) were negative and no positive RT-PCR results were recorded.
Conclusion:
The frequency of West Nile virus among renal transplant patients in Khartoum State –Sudan was documented through detection of specific IgM antibodies

Authors and Affiliations

Alia H Abaallh
Department of Microbiology, Faculty of Medical Laboratories, Al Neelain University, Khartoum, Sudan
Abdel Rahim M El Hussein
Department of Virology Central Laboratory - The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
Isam M Elkhidir
Department of Microbiology and Parasitology, University of Khartoum, Khartoum, Sudan
Khalid A Enan
Department of Virology Central Laboratory - The Ministry of Higher Education and Scientific Research, Khartoum, Sudan

West Nile Viruses (WNV), IgM .ELISA and Real-Time PCR.

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Publication Details

Published in : Volume 4 | Issue 4 | March-April 2018
Date of Publication : 2018-04-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 667-670
Manuscript Number : IJSRSET173852
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Alia H Abaallh, Abdel Rahim M El Hussein, Isam M Elkhidir, Khalid A Enan, " Prevalence and Molecular Detection of West Nile Virus (WNV) Among Renal Transplant Patients in Khartoum State, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 4, pp.667-670, March-April-2018.
Journal URL : http://ijsrset.com/IJSRSET173852

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