Molecular Detection of Human Herpes Virus types 1 and 2 Among pregnant women in Khartoum State, Sudan

Authors(5) :-Alaa Talaat, Abdel Rahim M El Hussein, Azza Babiker, Isam M Elkhidir, Khalid A Enan

Background:Infection with herpes simplex is one of the most common sexually transmitted infections. Because the infection is common in women of reproductive age it can be contracted and transmitted to the fetus during pregnancy and to the newborn. Herpes simplex virus is considered as an important cause of neonatal infection, which can lead to death or long-term disabilities. The greatest risk of transmission to the fetus and the newborn occurs in case of an initial maternal infection contracted in the second half of pregnancy. The risk of transmission of maternal-fetal-neonatal herpes simplex can be decreased by performing a treatment with antiviral drugs or resorting to a caesarean section in some specific cases. The purpose of this paper is to determine the prevalenceof HSV-1 and HSV-2 infections among pregnant women in Khartoum State using ELISA and Real-time PCR

Methods: Pregnant females suspected to have herpes simplex infection, were enrolled in the study. Ninghty serum samples were collected from such patients in different hospitals in Khartoum State.The sera were Tested  for Herpes simplex virus types 1 and2 specific immunoglobin (IgM) antibodies using enzyme-linked immunosorbent assay(ELISA), and Real-time PCR  was used for detection of  HSV1&2 DNA

Results :Out of the 90 sera tested, 4 patient’s (4.4%) showed HSV1  IgM, and  4 (4.4% ) HSV1 DNA and 7 patients (7.7%)  showed HSV2 IgM  with 6 samples (6.6%)showing HSV-2 DNA .

Conclusion: The prevalence of HSV1 and HSV2 in pregnant females in Khartoum State, was documented through the molecular detection of HSV1 and HSV2 antibodies and detection of HSV1DNA and HSV 2 DNA.  Further study using various diagnostic methods should be considered to determine the prevalence of HSV1 and HSV2 diseases at the national level.

Authors and Affiliations

Alaa Talaat
Department of Microbiology, Faculty of Medical Laboratories, University of Al-Neelain, Khartoum, Sudan
Abdel Rahim M El Hussein
Department of Virology, Central Laboratory, Ministry of High Education and Scientific Research, Khartoum, Sudan
Azza Babiker
Department of Virology, Central Laboratory, Ministry of High Education and Scientific Research, Khartoum, Sudan
Isam M Elkhidir
Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
Khalid A Enan
Department of Virology, Central Laboratory, Ministry of High Education and Scientific Research, Khartoum, Sudan

ELISA, Real-time PCR, Sudan, Herpes simplex, Pregnancy.

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Publication Details

Published in : Volume 4 | Issue 10 | September-October 2018
Date of Publication : 2018-10-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 324-328
Manuscript Number : IJSRSET1841066
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Alaa Talaat, Abdel Rahim M El Hussein, Azza Babiker, Isam M Elkhidir, Khalid A Enan, " Molecular Detection of Human Herpes Virus types 1 and 2 Among pregnant women in Khartoum State, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 10, pp.324-328, September-October-2018. Available at doi : https://doi.org/10.32628/18410IJSRSET
Journal URL : http://ijsrset.com/IJSRSET1841066

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