Molecular Detection of Human Herpes Virus types 1 and 2 Among pregnant women in Khartoum State, Sudan
DOI:
https://doi.org//10.32628/18410IJSRSETKeywords:
ELISA, Real-time PCR, Sudan, Herpes simplex, Pregnancy.Abstract
Background : Infection with herpes simplex is one of the most common sexually transmitted infections. Because the infection is common in women of reproductive age it can be contracted and transmitted to the fetus during pregnancy and to the newborn. Herpes simplex virus is considered as an important cause of neonatal infection, which can lead to death or long-term disabilities. The greatest risk of transmission to the fetus and the newborn occurs in case of an initial maternal infection contracted in the second half of pregnancy. The risk of transmission of maternal-fetal-neonatal herpes simplex can be decreased by performing a treatment with antiviral drugs or resorting to a caesarean section in some specific cases. The purpose of this paper is to determine the prevalenceof HSV-1 and HSV-2 infections among pregnant women in Khartoum State using ELISA and Real-time PCR
Methods: Pregnant females suspected to have herpes simplex infection, were enrolled in the study. Ninghty serum samples were collected from such patients in different hospitals in Khartoum State.The sera were Tested for Herpes simplex virus types 1 and2 specific immunoglobin (IgM) antibodies using enzyme-linked immunosorbent assay(ELISA), and Real-time PCR was used for detection of HSV1&2 DNA
Results :Out of the 90 sera tested, 4 patient’s (4.4%) showed HSV1 IgM, and 4 (4.4% ) HSV1 DNA and 7 patients (7.7%) showed HSV2 IgM with 6 samples (6.6%)showing HSV-2 DNA .
Conclusion: The prevalence of HSV1 and HSV2 in pregnant females in Khartoum State, was documented through the molecular detection of HSV1 and HSV2 antibodies and detection of HSV1DNA and HSV 2 DNA. Further study using various diagnostic methods should be considered to determine the prevalence of HSV1 and HSV2 diseases at the national level.
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