Serological and Molecular Detection of Chikungunya Virus among Arthritis Patient in Khartoum State, Sudan

Authors(5) :-Eman M Tatay, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan

Background : Noroviruses (NoVs) are human pathogens associated with acute viral gastroenteritis worldwide and an important cause of childhood morbidity and mortality in developing countries. However, there are still few epidemiological data on the occurrence of these viruses in Sudan. This study was conducted to investigate the molecular epidemiology of NoVs in children less than 5 years with acute gastroenteritis.

Methods: A total of 66 stool samples were collected from children under five years of age presenting with acute gastroenteritis, in Omdurman teaching hospital and Aljazeera teaching hospital, during the period from January to May2017. For the detection of norovirus, total RNA was extracted from all samples, followed by Real time polymerase chain reaction (RT-PCR) Detected noroviruses were then genogrouped.

Results: Out of the 66 samples tested, one sample in Khartoum and 18 samples in Aljazeera were positive for norovirus infections by RT-PCR. The dected noroviruse - positive samples belonged GII and GI genogroups but the dominant genotype was GII.

Conclusions: The present study showed that norovirus are an important causative agents of gastroenteritis in children less than 5 years .There is a great need for introducing routine norovirus testing of hospitalized children with gastroenteritis.

Authors and Affiliations

Eman M Tatay
Department of Medical microbiology, Faculty of Medical Laboratory Science, El Nellin University, Khartoum, Sudan
Abdel Rahim M El Hussein
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
Mohamed O. Mustafa
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
Isam M Elkhidir
Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
Khalid A Enan
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan

Chikungunya virus, ELISA , anti ccp, Reverse transcriptase Real time PCR .

  1. Robinson MC. An epidemic of virus disease in Southern Province,Tanganyika Territory in 1952–53—1: clinical features. Trans R Soc Trop Med Hyg. 1955;49:28.
  2. Tsetsarkin KA, Vanlandingham DL, McGee CE, Higgs S: A single mutation in chikungunya virus affects vector specificity and epidemic potential. PLoSPathog. 2007, 3:e201.
  3. Thiberville SD, Moyen N, Dupuis-Maguiraga L, et al. Chikungunya fever: epidemiology, clinical syndrome, pathogenesis and therapy. Antivir Res. 2013;99:345–70.
  4. Schilte C, Staikowsky F, Couderc T, et al. Chikungunya virusassociated long-term arthralgia: a 36 month prospective longitudinal study. PLoS Negl Trop Dis. 2013;7, e2137. doi:10.1371/ journal.pntd.0002137.
  5. Mathew AJ, Goyal V, George E, Thekkemuriyil DV, Jayakumar B,Chopra A. Rheumatic-musculoskeletal pain and disorders in anaïve group of individuals 15 months following a Chikungunya viral epidemic in south India: a population based observational study. Int J Clin Pract. 2011;65:1306–12.
  6. Borgherini G, Poubeau P, Staikowsky F, et al. Outbreak of chikungunyaon Réunion Island: early clinical and laboratory features in 157 adult patients.ClinInfectDis.Jun1 2007;44(11):1401-1407.
  7. Taubitz W, Cramer JP, Kapaun A, et al. Chikungunya fever in travelers: clinical presentation and course. Clin Infect Dis. Jul 1 2007;45(1):e1-4.
  8. Jupp PG, McIntosh BM. Chikungunya virus disease. In: Monath TP, ed. The Arboviruses: Epidemiology and Ecology. Vol II. Boca Raton, FL: CDC Press, Inc.; 1988.
  9. Ryman KD, Klimstra WB. Closing the gap between viral and noninfectious arthritis. Proc Natl Acad Sci U S A. 2014;111:5767–8.
  10. Adam A, Seidahmed OM, Weber C, Schnierle B, Schmidt-Chanasit J, Reiche S, Jassoy C. Low Seroprevalence Indicates Vulnerability of Eastern and Central Sudan to Infection with Chikungunya Virus. Vector-Borne and Zoonotic Diseases. 2016 Apr 1;16(4):290-1.
  11. Mohamed HM Isa, Abbas B Mohammed,Wafa I Elhag : Serofrequency of Chikungunya Virus Among Blood Donors in Singa City Sinnar State, Sudan 2017; International Journal of Innovative Science and Research Technology Vol 2 (12). Dec 2017 . 165-169 .
  12. Sissoko D, Moendandze A, Malvy D, Giry C, Ezzedine K, Solet J-L,Pierre V: Seroprevalence and risk factors of chikungunya virusinfection in Mayotte, Indian Ocean, 2005-2006: a populationbased survey. PLoS ONE 2008, 3:e3066.
  13. Lokireddy S, Vemula S, Vadde R: Connective tissue metabolism in Chikungunya patients. Virol J 2008, 5:31.
  14. Adam A, Seidahmed OM, Weber C, Schnierle B, Schmidt-Chanasit J, Reiche S, Jassoy C. Low Seroprevalence Indicates Vulnerability of Eastern and Central Sudan to Infection with Chikungunya Virus. Vector-Borne and Zoonotic Diseases. 2016 Apr 1;16(4):290-1.
  15. Gould LH, Osman MS, Farnon EC, Griffith KS, Godsey MS, Karch S, Mulenda B, El Kholy A, Grandesso F, de Radiguès X, Brair ME. An outbreak of yellow fever with concurrent chikungunya virus transmission in South Kordofan, Sudan, 2005. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2008 Dec 1;102(12):1247-54.
  16. Ahmed Y Rodwan,Abbas Bm Rahama, Rania S Ahmed, Nazar M Abdalla, Amani A Osman, Mustafa E Yassin: Seroprevalence of Chikungunya virus within arthritis patients in Khartoum, Sudan 2016. European Academic Research Vol. IV, June 2016. 2210- 2220 .
  17. Moyen N,Thiberville S-D,Pastorino B,Nougairede A, Thirion L,Mombouli J-V et al .(2014) Frist Reported Chikungunya Fever Outbreak in the Republic of Congo,2011. PLoS ONE 9 (12):e 115938. Doi:10.1371.

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Publication Details

Published in : Volume 4 | Issue 9 | July-August 2018
Date of Publication : 2018-08-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 458-462
Manuscript Number : IJSRSET184414
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Eman M Tatay, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Serological and Molecular Detection of Chikungunya Virus among Arthritis Patient in Khartoum State, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 9, pp.458-462, July-August-2018.
Journal URL : http://ijsrset.com/IJSRSET184414

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