Molecular Detection of Norovirus 1, 2 in Children Less than 5 Years with Gastroenteritis in Khartoum and Al Jazeera States, Sudan

Authors

  • Eman M Tatay  Department of Medical microbiology, Faculty of Medical Laboratory Science, El Neelain University, Khartoum, Sudan
  • Abdel Rahim M El Hussein  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
  • Mohamed O. Mustafa  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
  • Isam M Elkhidir  Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Khalid A Enan  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan

Keywords:

NoVs, RT-PCR, Norovirus, Khartoum, Al Jazeera, Sudan

Abstract

Background: Noroviruses (NoVs) are human pathogens associated with acute viral gastroenteritis worldwide and an important cause of childhood morbidity and mortality in developing countries. However, there are still few epidemiological data on the occurrence of these viruses in Sudan. This study was conducted to investigate the molecular epidemiology of NoVs in children less than 5 years with acute gastroenteritis.
Methods: A total of 66 stool samples were collected from children under five years of age presenting with acute gastroenteritis, in Omdurman teaching hospital and Aljazeera teaching hospital, during the period from January to May2017. For the detection of norovirus, total RNA was extracted from all samples, followed by Real time polymerase chain reaction (RT-PCR) Detected noroviruses were then genogrouped.
Results: Out of the 66 samples tested, one( 5.3%) sample in Khartoum and 18 (94.7%) samples in Aljazeera were positive for norovirus infections by RT-PCR. The detected noroviruse - positive samples belonged to GII and GI genogroups but the dominant genotype was GII.
Conclusions: The present study showed that norovirus are an important causative agents of gastroenteritis in children less than 5 years .There is a great need for introducing routine norovirus testing of hospitalized children with gastroenteritis.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-08-30

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Volume 4, Issue 9, July-August-2018

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Research Articles

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[1]
Eman M Tatay, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Molecular Detection of Norovirus 1, 2 in Children Less than 5 Years with Gastroenteritis in Khartoum and Al Jazeera States, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 9, pp.458-462, July-August-2018.