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Neuroprotective Effects of Asparagus racemosus Willd and Withania Somnifera Dunal: An In Vitro And In Vivo Study

Authors(1):

Durgesh Sharma
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Aims of the study   was to investigate and compare  the  protective effects of  two plants Asparagus racemosus  Willd  (AR) and Withania somnifera Dunal (WS) in  in vitro and in vivo  models of neurodegeneration.  Further, it was also tested that the protective action was due to antioxidant activity of both the plants.  In vitro  study was carried out in hippocampal neuron cells in primary culture  and in  vivo in young adult  Wistar rats.  Root   extracts  of A.racemosus and  W.somnifera  were obtained from a  authorized supplier.  To asses  neuroprotective  action  of  the plants,  cell viability tests ( AO/EB staining, MTT assay, LDH leakage assay), extracellular Free Ca2+,  ROS determination, GSH-GSSG ratio  and  lipid peroxidation were studied in  in vitro.  In  in vivo  study,  ROS determination,  Free Ca2+,  GSH-GSSG ratio, GPx level and lipid peroxidation were determined in hippocampal tissue.  In addition dose response and exposure time response was also studied in in vitro.  Results suggested that in culture, significant decrease in dead cells was observed after   treatment with WS as compared to   AR+WS.  MTT assay also confirmed these observations. A significant decrease in Free Ca2+ ,   ROS and lipid peroxidation and increase in GSH-GSSG ratio was observed.  In  hippocampal tissue as well ,  a significantly decrease in  ROS level,  Free Ca2+,  GPx level and lipid peroxidation  but increase in GSH-GSSG ratio  was observedStudy thus demonstrates that AR and WS both have potent neuroprotective action and that   this effect is due to their antioxidant properties

Durgesh Sharma

A.racemosus, Antioxidants, Hippocampal cell culture, Neuroprotection, W.somnifera

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Publication Details

Published in : Volume 1 | Issue i | November-December - 2014
Date of Publication Print ISSN Online ISSN
2014-04-30 2395-1990 2394-4099
Page(s) Manuscript Number   Publisher
111-121 IJSRSET1844271   Technoscience Academy

Cite This Article

Durgesh Sharma, "Neuroprotective Effects of Asparagus racemosus Willd and Withania Somnifera Dunal: An In Vitro And In Vivo Study", International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 1, Issue i, pp.111-121, November-December-2014.
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