Detection of West Nile Virus in Hospitalized Children with Acute Meningitis using Reverse Transcription Loop-Mediated Isothermal Amplification in Khartoum state, Sudan

Authors

  • Roua A Osman  Department of Medical microbiology, Faculty of Medical Laboratory Science, El Nellin University, Khartoum, Sudan
  • Abdel Rahim M El Hussein  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
  • Mohamed O. Mustafa  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
  • Isam M Elkhidir  Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Khalid A Enan  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan

Keywords:

West Nile Virus, aseptic meningitis, Real Time RT-LAMP Sudan

Abstract

Background: This study was carried out to detect West Nile virus (WNV) among hospitalized children with acute aseptic meningitis in Khartoum State, Sudan.
Methods: Cerebrospinal fluid specimens were collected from 70 aseptic meningitis patients from Mohammed Alamin Hamid Hospital during the period January to May 2017.
Out of these 70 patients, 41 (59%) were males and 29 (41%) were females with age that ranged between 1 and 24 months.
The collected CSF specimens were  subjected to Real time LAMP for detection of WNV RNA.
Results:  Out of 70 patients, 14 (20 %) were positive using Real time LAMP   to WNV RNA,, 9 from them were males and the other 5 were females.
Conclusions: In Sudan, the existence   of WNV in aseptic meningitis patients was confirmed through detection of WNV RNA. Further study using various diagnostic methods should be considered to determine the prevalence of WNV at the national level.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-06-30

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Volume 4, Issue 8, May-June-2018

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Research Articles

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[1]
Roua A Osman, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Detection of West Nile Virus in Hospitalized Children with Acute Meningitis using Reverse Transcription Loop-Mediated Isothermal Amplification in Khartoum state, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 8, pp.659-663, May-June-2018.