Molecular Detection of Rotavirus (A and B) and Astrovirus in Children Less than 5 Years with Gastroenteritis in Khartoum and Aljazeera States, Sudan

Authors

  • Einas E Mohamed  Department of Medical microbiology, Faculty of Medical Laboratory Science, El Nellin University, Khartoum, Sudan
  • Abdel Rahim M E Hussein  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
  • Mohamed O. Mustafa  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
  • Isam M Elkhidir  Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Khalid A Enan  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan

DOI:

https://doi.org//10.32628/18410IJSRSET

Keywords:

Rotavirus, Astrovirus, Diarrhea, Vomiting.

Abstract

Background: Diarrheal diseases represent a major worldwide public health problem particularly in developing countries. Each year, at least four million children under five years of age die from diarrhoea. Although rotavirus is a leading cause, other viruses including astroviruses are also important, but have been the subject of limited studies. The objective of this study was to estimate the Rotavirus and astrovirus gastroenteritis among children less than 5 years.

Methods: This study was an active surveillance cross sectional study in a total of 66 diarrhoea specimens collected from children of less than 5 years old with gastroenteritis in Aljazeera Children’s Hospital and Omdurman children’s Hospital during January to May 2017. RNA was extracted from all specimens, followed by Real Time-PCR amplifications to detect rotavirus and astrovirus were performed.

Results: of the 66 samples tested for rota virus, one sample was positive in Aljazeera samples (1,5%) while no positives were detected in Khartoum samples. While for astrovirus there was one positive in Khartoum samples (1.5%) and one positive in samples from AlJazeera (1.5%). 

Conclusion: The current study showed that infection with astroviruses may be as an important cause of gastroenteritis as rotavirus. These findings highlight the need to implement rotavirus and astrovirus detection assays in clinical diagnosis to prevent nosocomial spread of viral gastroenteritis infections in paediatric departments. It is recommended to conduct genotyping of rotavirus on large samples before starting vaccination in the country.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-09-30

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Volume 4, Issue 10, September-October-2018

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Research Articles

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How to Cite

[1]
Einas E Mohamed, Abdel Rahim M E Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Molecular Detection of Rotavirus (A and B) and Astrovirus in Children Less than 5 Years with Gastroenteritis in Khartoum and Aljazeera States, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 10, pp.07-13, September-October-2018. Available at doi : https://doi.org/10.32628/18410IJSRSET