Molecular Detection of Astrovirus, Rotavirus (A and B) in Children Less than 5 Years with Gastroenteritis in Aljazeera State, Sudan

Authors(5) :-Einas E Mohamed, Abdel Rahim M E Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan

Background: Diarrheal diseases represent a major worldwide public health problem particularly in developing countries. Each year, at least four million children under five years of age die from diarrhea. Although rotavirus is a leading cause, other viruses including astrovirus are also important, but have been the subject of limited studies. The objective of this study was to estimate the Rotavirus and astrovirus gastroenteritis among children less than 5 years.

Methods: This study was active surveillance cross sectional study included a total of 66 diarrhea specimens collected from children less than 5 years with gastroenteritis in   Aljazeera Children’s Hospital and Omdurman children  Hospital during  January to May2017.From all specimens RNA was extracted, followed by Real Time-PCR amplification to detected Rotavirus and astrovirus was performed. Results:

From 66 samples, one positive in Aljazeera samples (1,5%) while no positive in Khartoum samples for Rotavirus infection by RT-PCR. While for Astrovirus there is one positive in Aljazeera samples (1.5%) and one positive in Khartoum samples (1.5%) for Astrovirus infection by RT-PCR.

Conclusion: The current study showed that infection with astroviruses may be an important cause of gastroenteritis, as well as rotavirus, these findings highlight the need to implement rotavirus and astrovirus detection assays in clinical diagnosis and the nosocomial prevention of gastroenteritis viral infections in pediatric departments. It is recommended to conduct genotyping of Rotavirus on large samples before starting vaccination in the country.

Authors and Affiliations

Einas E Mohamed
Department of Medical microbiology, Faculty of Medical Laboratory Science, El Nellin University, Khartoum, Sudan
Abdel Rahim M E Hussein
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
Mohamed O. Mustafa
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
Isam M Elkhidir
Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
Khalid A Enan
Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan

Rotavirus, Astrovirus, Diarrhea, Vomiting.

  1. Parkin, PC., CMacarthur, AKhambalia, RDGoldman, and JN.Friedman1 June 2009Clinical and laboratory assessment of dehydration severity in children With acute gastroenteritisClinPediatr49:235–239[Epub ahead of print.]
  2. GBD 2013 Mortality and Causes of Death CollaboratorsGlobal, regional, and national agesex specific allcause and causespecific mortality for 240 causes of death, 1990–2013: a systematic analysis for the Global Burden of Disease Study 2013Lancet2014Epub 2014/12/23doi: 10.1016/s01406736 (14)616822 PMID: 25530442.
  3. Walter JE, Mitchell DKAstrovirus infection in childrenCurr Opin Infect Dis.2003;16(3):247–53.
  4. Mendez E, Arias CFAstrovirusesIn: Knipe DM, Howley PM, Cohen JI, Griffin DE, Lamb RA, Martin MA, Racaniello VR, Roizman B, editorsFields virology5th edPhiladelphia: LippincottWilliams and Wilkins; 2007p981–1000.
  5. Astrovirus Gastroenteritis in Children in Taipei HsiaoChuan Lin,1 ChuanLiang Kao,2 LuanYin Chang,3 YuChia Hsieh,3 PeiLan Shao,3 PingIng Lee,3 ChunYi Lu,3 ChinYun Lee,3 LiMin Huang3.
  6. Bosch A, Pintó RM, Guix SHuman astrovirusesClin Microbiol Rev2014; 27(4):1048–74.
  7. Nguyen TA, Hoang L, le Pham D, Hoang KT, Mizuguchi M, Okitsu S, et alIdentification of human astrovirus infections among children with acute gastroenteritis in the Southern Part of Vietnam during 2005–2006J Med Virol2008;80(2):298–305. 2009/Accepted 25 February 2010.
  8. Black RE, Cousens S, Johnson HL, Lawn JE, Rudan I, Bassani DG, Jha P,Campbell H, Walker CF, Cibulskis R, et al: Global, regional, and nationalcauses of child mortality in 2008: a systematic analysisLancet 2010,375(9730):1969–1987.
  9. Widdowson MA, Bresee JS, Gentsch JR, Glass RI: Rotavirus disease and itspreventionCurr Opin Gastroenterol 2005, 21:26–31.
  10. Bines JE: Rotavirus vaccines and intussusception riskCurr OpinGastroenterol 2005, 21:20–25.
  11. Kane EM, Turcios RM, Arvay ML, Garcia S, Bresee JS, Glass RI: Theepidemiology of rotavirus diarrhea in Latin AmericaAnticipatingrotavirus vaccinesRev Panam Salud Publica 2004, 16:371–377.
  12. WHO: Rotavirus and other viral diarrheasBull WHO 2007, 58(2):183–198.
  13. Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI: Global illness and deaths caused by rotavirus disease in childrenEmerg Infect Dis 2003,9(5):565–572.
  14. Diggle L: Rotavirus diarrhoea and future prospects for preventionBr JNurs 2007, 16(16):970–974.
  15. Grimwood K, Lambert SB: Rotavirus vaccines: opportunities andchallengesHum Vaccin 2009, 5(2):57–69.
  16. Parashar UD, Gibson CJ, Bresse JS, Glass RI: Rotavirus and severe childhood diarrheaEmerg Infect Dis 2006, 12(2):304–306.
  17. Guerrant RL, Hughes JM, Lima NL, Crane J: Diarrhea in developed anddeveloping countries: magnitude, special settings, and etiologiesRevInfect Dis 1990, 12(Suppl 1):S41–S50.
  18. de Wit, MA., MPKoopmans, LMKortbeek, WJWannet, JVinje, F.van Leusden, AIBartelds, and YTvan Duynhoven2001Sensor, apopulationbased cohort study on gastroenteritis in the Netherlands: incidenceand etiologyAmJEpidemiol154:666–674
  19. Levidiotou, S., CGartzonika, DPapaventsis, CChristaki, EPriavali, N.Zotos, EKapsali, and GVrioni2009Viral agents of acute gastroenteritis in hospitalized children in GreeceClinMicrobiolInfect15:596–598
  20. Oh, D.Y., GGaedicke, and ESchreier2003Viral agents of acute gastroenteritis in German children: prevalence and molecular diversityJMedVirol71:82–93
  21. Mertens TE, Wijenayake R, Pinto MR, Peiris JS, Wijesundera MD, Eriyagama NB, Karunarathne KG, Ranaweera LR: Microbiological agents associated with childhood diarrhea in the dry zone of Sri LankaTrop Med Parasitol 1990, 41(1):115–120.
  22. McIver CJ, Hansman G, White P, Doultree JC, Catton M, Rawlinson WD: Diagnosis of enteric pathogens in children with gastroenteritisPathology 2001, 33:353–358.
  23. Lopman BA, Reacher MH, Duijnhoven V, Hanon YF, Brown XD, Koopmans M: Viral gastroenteritis outbreaks in Europe, 1995–2000Emerg Infect Dis 2003, 9:90–96.
  24. Simpson R, Aliyu S, IturrizaGomara M, Desselberger U, Gray J: Infantile viral gastroenteritis: on the way to closing the diagnostic gapJ Med Virol 2007, 70:258–262.
  25. Parashar UD, Breseee JS, Gentsch JR, Glass RI: RotavirusEmerg Infect Dis 1998, 4:561–570.
  26. Bryce J, BoschiPinto C, Shibuya K, Black REWHO estimates of the causes of death in childrenLancet2005; 365 (9465): 114752
  27. Sixl W, SixlVoigt B, Stünzner D, Arbesser C, Reinthaler F, Mascher F, Rosegger H, Schneeweiss W, Schuhmann GInvestigations in the problem of diarrhoea in the Melut district, South Sudan (19811982)J Hyg Epidemiol Microbiol Immunol1987; 31(4): 4869
  28. Trabelsi A, Peenze I, Pager C, Jeddi M, Steele DDistribution of rotavirus VP7 serotypes and VP4 genotypes circulating in Sousse, Tunisia from 1995 to 1999: emergence of natural human reassortantsJ Clin Microbiol2000; 38(9):34159
  29. Kiulia NM, Peenze I, Dewar J, Nyachieo A, Galo M, Omolo E, Steele AD, Mwenda JMMolecular characterisation of the rotavirus strains prevalent in Maua, Meru North, KenyaEast Afr Med J2006; 83(7): 360365
  30. Rahouma A, Klena JD, Krema Z, Abobker AA, Treesh K, Franka E, Abusnena O, Shaheen HI, El Mohammady H, Abudher A, Ghenghesh KSEnteric pathogens associated with childhood diarrhea in TripoliLibyaAm J Trop Med Hyg2011; 84(6): 886891
  31. Monastiri et alBMC Public Health (2016) 16:57 DOI 10.1186/s1288901627265.
  32. Bosch A, Pintó RM, Guix SHuman astrovirusesClin Microbiol Rev2014; 27(4):1048–74.

Publication Details

Published in : Volume 4 | Issue 10 | September-October 2018
Date of Publication : 2018-09-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 07-13
Manuscript Number : IJSRSET184473
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Einas E Mohamed, Abdel Rahim M E Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Molecular Detection of Astrovirus, Rotavirus (A and B) in Children Less than 5 Years with Gastroenteritis in Aljazeera State, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 10, pp.07-13, September-October.2018
URL : http://ijsrset.com/IJSRSET184473

Follow Us

Contact Us