In Vitro Biofilm Study in Tetracycline Resistant Escherichia Coli from Mastitis Samples

Abstract

Antimicrobial resistance is a global concern in both human and veterinary medicine as there is therapeutic failure for infectious disease. Every year millions of people dying worldwide because of multiple drug resistance (MDR). Multiple drug resistant bacteria and genes can transfer between humans and animals. Transfer of MDR bacteria and genes form food producing animals to humans has great contribution in spread and occurrence of MDR. Drug resistance in mastitis producing organism is very common due to indiscriminate use of antibiotics in the treatment of mastitis. Among various resistance mechanisms biofilm formation of antibiotic resistance is gaining importance as mastitis causing organism are more prone to biofilm production due to availability of raw materials like lactose for biofilm synthesis and causing therapeutic failure. In this study biofilm is assayed by simplest method called congo red dye assay in which congo red dye is used and sucrose is added as energy source. Six tetracycline resistant E. coli strains are analysed for biofilm and all E coli isolates from mastitis milk were negative for biofilm production. E coli used in this study failed to produce biofilm may be because involvement of other resistant mechanisms like altered binding site, Efflux pump and inactivation of antibiotics by enzymes.