Determination of Various Parameters of Crude and Partially Purified Dialysed Pectinase Enzyme Produced from Aspergillus Niger Using Pineapple Peels

Authors

  • B. Leelamani  Research Scholar of Rayalaseema University, Kurnool, Andhra Pradesh, India
  • Dr. D. Sailaja  Professor, Dean, EDC, Gokaraju Rangaraju College of Engineering and Technology. Hyderabad, Telangana, India
  • Dr. K. Kamala  Assistant Professor and Coordinator, Rayalaseema University.Kurnool, Andhra Pradesh, India

Keywords:

Pineapple Peels, Pectin, Aspergillus niger, Pectinase and Partial Purification.

Abstract

The agricultural wastes generated from pineapple (Ananas cosmosus) represents about 35% of the entire fruit. These wastes can be converted to most useful products such as pectin. Pectin was extracted from pineapple peels with a percentage yield of 8.33% at pH 2.2 and temperature of 70° C. Three pectinolytic fungi: Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus were isolated from natural sources and tested for their pectinolytic activity. In submerged fermentation system containing pineapple pectin broth, Aspergillus niger was inoculated and incubated for 4 days. The crude enzyme pectinase was harvested after fermentation by filteration process. Crude enzyme obtained from Aspergillus niger was precipitated with 80% ammonium sulphate saturation. The dialysis is carried out according to Dixon and Webb (1964) for 12 hours to obtain partially purified enzyme. Then the protein concentration and activities of the crude and dialyzed pectinase enzymes were compared.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-08-30

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Volume 4, Issue 9, July-August-2018

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Research Articles

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[1]
B. Leelamani, Dr. D. Sailaja, Dr. K. Kamala, " Determination of Various Parameters of Crude and Partially Purified Dialysed Pectinase Enzyme Produced from Aspergillus Niger Using Pineapple Peels, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 9, pp.538-549, July-August-2018.