Determination of Various Parameters of Crude and Partially Purified Dialysed Pectinase Enzyme Produced from Aspergillus Niger Using Pineapple Peels

Authors(3) :-B. Leelamani, Dr. D. Sailaja, Dr. K. Kamala

The agricultural wastes generated from pineapple (Ananas cosmosus) represents about 35% of the entire fruit. These wastes can be converted to most useful products such as pectin. Pectin was extracted from pineapple peels with a percentage yield of 8.33% at pH 2.2 and temperature of 70° C. Three pectinolytic fungi: Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus were isolated from natural sources and tested for their pectinolytic activity. In submerged fermentation system containing pineapple pectin broth, Aspergillus niger was inoculated and incubated for 4 days. The crude enzyme pectinase was harvested after fermentation by filteration process. Crude enzyme obtained from Aspergillus niger was precipitated with 80% ammonium sulphate saturation. The dialysis is carried out according to Dixon and Webb (1964) for 12 hours to obtain partially purified enzyme. Then the protein concentration and activities of the crude and dialyzed pectinase enzymes were compared.

Authors and Affiliations

B. Leelamani
Research Scholar of Rayalaseema University, Kurnool, Andhra Pradesh, India
Dr. D. Sailaja
Professor, Dean, EDC, Gokaraju Rangaraju College of Engineering and Technology. Hyderabad, Telangana, India
Dr. K. Kamala
Assistant Professor and Coordinator, Rayalaseema University.Kurnool, Andhra Pradesh, India

Pineapple Peels, Pectin, Aspergillus niger, Pectinase and Partial Purification.

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Publication Details

Published in : Volume 4 | Issue 9 | July-August 2018
Date of Publication : 2018-08-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 538-549
Manuscript Number : IJSRSET184990
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

B. Leelamani, Dr. D. Sailaja, Dr. K. Kamala, " Determination of Various Parameters of Crude and Partially Purified Dialysed Pectinase Enzyme Produced from Aspergillus Niger Using Pineapple Peels, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 9, pp.538-549, July-August-2018.
Journal URL : http://ijsrset.com/IJSRSET184990

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