Production and characterization of the biosurfactant obtained by Bacillus subtilis
Keywords:
Biosurfactants, FT-IR, CMC and Bacillus subtilisAbstract
Biosurfactants are surface active amphiphilic compounds with effective surface-active and biological properties applicable to several industries and processes. In this study, isolation and identification of biosurfactant producing strain were assessed. Soil samples from petrol pumps in Kalyan and Ulhasnagar area was collected and 38 strains were isolated. To confirm the ability of isolates to produce biosurfactant, emulsification assay, E24 test and surface tension measurement tests were performed. Bacillus subtilis isolated from petrol pump station, was used to produce biosurfactant using a modified mineral salt medium with 2% diesel as sole source of carbon. The chemical composition of the biosurfactant was qualitatively analyzed by thin layer chromatography. The FT-IR analysis revealed the lipopeptide nature of the biosurfactant. The surface tension measurement using Kruss processor tensiometer showed the CMC of 140 mgL-1. The biosurfactant was found to be quite stable under varying conditions of temperature and pH.
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