Study of Antibacterial activity of Mulethi and Amla against gram negative bacteria Escherichia coli

Abstract

Present investigation is performed on comparative study of antibacterial potential of two plants viz., Glycyrrhiza glabra and Emblica officinalis commonly known as Mulethi and Amla respectively, against the gram negative bacteria Escherechia coli. E.coli is a well-known and a commonly used bacterium which is a useful tool for genetic research because of its relatively small size, easy availability and ability to grow rapidly under conducive situations. Glycyrrhiza glabra is commonly used as condiment in sweets, teas, soft drinks and tobacco products. It not only acts as a good flavouring agent but is also widely used home remedy because of its medicinal properties. This medicinal herb has wide reaching health benefits in naturally treating sore throat, chest congestion, rheumatoid arthritis, strengthening of bones and muscles, kidney problems, mouth ulcers, etc. According to Ayurveda, Emblica officinalis balances all the three fundamental bio-elements of the body (viz., pitta, vatta and kapha). Apart from its ability to soothe digestion, Amla may be used as a rejuvenating agent, anti-inflammatory agent, and anti-diabetic agent, helps to reduce fever, stimulate hair growth and enhance intellect. Aqueous and crude extracts of these plants were prepared to find their antibacterial efficacy. The crude extracts of Mulethi and Amla -exhibited greater antibacterial properties against the bacteria, as confirmed with the help of antibacterial assays. The growth inhibition of bacteria was studied using growth curve assay at 660 nm using Elico CL63 photometer. Well diffusion assay was performed for 1%, 10% and 100% crude extracts for either plant materials and it was found that 100% extract had maximum antibacterial effect.