Protease Production Using Solid State Fermentation

Authors

  • Dhaliwal MK  Department of Microbiology, Birla College, Kalyan, Maharashtra, India
  • Patil RN  Department of Microbiology, Birla College, Kalyan, Maharashtra, India
  • Sambare S  Department of Microbiology, Birla College, Kalyan, Maharashtra, India

Keywords:

Bioprocess, Protease, Solid State Fermentation, Bran, Optimization.

Abstract

Proteases are enzymes that break down protein molecules through peptide bond hydrolysis. Microbial proteases are the most important industrial enzymes with considerable applications in food, medicines and pharmacy. They are extracellular enzymes that can be produced by both submerged and solid state fermentation (SSF). Aspergillus sp. are known to produce various types of proteases.  Solid state fermentation processes generally use natural raw materials as carbon and energy source. Different agro-industrial waste products will be evaluated to check possibility of potential utilization of substrates in SSF. The production of enzymes by bioprocesses is a good value added to agro industry residues. A comparative study was carried out on the production of protease using Wheat bran and Rice bran as substrates in solid state fermentation by Aspergillus niger ATCC 16404. Among them Wheat bran produced higher activity protease as 1.785 U/ml than Rice bran 1.487 U/ml activity under solid state fermentation conditions. The optimized conditions for producing maximum yield of protease were incubation at room temperature (28±2)°C, 120hours, pH 6, concentration of nitrogen source 0.5% and additional carbon source is 1% sucrose. The protease production from agricultural waste products can be commercially used in detergents and leather industry and it adds great value to agro-industrial wastes.<

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-02-28

Issue

Volume 5, Issue 4, January-February-2018

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Research Articles

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How to Cite

[1]
Dhaliwal MK, Patil RN, Sambare S, " Protease Production Using Solid State Fermentation, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 5, Issue 4, pp.80-88, January-February-2018.