Green synthesis of silver nanoparticles from plant sources and evaluation of their antimicrobial activity

Authors(7) :-Sapna G. Yadav, Sudeep H Patil, Pratima Patel, Vineeetha Nair , Shahida Khan, Shivani Kakkar , Annika Durve Gupta

Nanoparticles are used widely due to its small size, orientation, physical properties, which are reportedly shown to change the performance of any other material which is in contact with these particles. Silver nanoparticles have received substantial attention in the field of antimicrobial research due to the antimicrobial activity of silver. These particles can be synthesized easily by different chemical, physical, and biological approaches. The biological approach is the most emerging approach of preparation, as this method is easier than the other methods, ecofriendly, non toxic and less time consuming. In the present study green synthesis of silver nanoparticles was carried out using the plant extracts of Neem (Azadirachta indica), Tea (Camellia sinensis), Peels from kitchen waste and the mixture of all the three extracts. Silver was of a particular interest for this process due to its evocative physical and chemical properties. The reduction process of Ag+ to Ag0 was observed by formation of brown colour within 24 hours of incubation period and synthesized SNPs showed an absorption peak at around 450 nm in the UV-visible spectrum. The reaction was followed by the characterization of the silver nanoparticles using UV-Vis and FTIR. The antimicrobial activity was tested using agar well method with Gram positive and Gram negative microorganism. It was observed that the antimicrobial activity was higher against Gram negative bacteria. Local plants such as tea leaves and waste is an alternative to a safer, more eco-friendly alternatives of synthesizing silver nanoparticles. This environment friendly, method provides simple, easy and cost effective faster synthesis of nanoparticles than chemical method and can be used in several areas such as catalysis, medical application etc.

Authors and Affiliations

Sapna G. Yadav
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.
Sudeep H Patil
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.
Pratima Patel
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.
Vineeetha Nair
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.
Shahida Khan
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.
Shivani Kakkar
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.
Annika Durve Gupta
Department of biotechnology, university of Mumbai, Kalyan, Maharashtra, India.

Silver nanoparticles, UV-Visible Spectroscopy, FTIR, Antimicrobial activity

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Publication Details

Published in : Volume 5 | Issue 4 | January-February 2018
Date of Publication : 2018-02-28
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 133-139
Manuscript Number : IJSRSET185425
Publisher : Technoscience Academy

Print ISSN : 2395-1990, Online ISSN : 2394-4099

Cite This Article :

Sapna G. Yadav, Sudeep H Patil, Pratima Patel, Vineeetha Nair , Shahida Khan, Shivani Kakkar ,Annika Durve Gupta, " Green synthesis of silver nanoparticles from plant sources and evaluation of their antimicrobial activity, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 5, Issue 4, pp.133-139, January-February-2018. Citation Detection and Elimination     |     
Journal URL : https://ijsrset.com/IJSRSET185425

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