Polymorphism Analysis of TGF-β2 Gene and Its Association with Body Weight and Body Size Measurements of Tolaki Chicken
DOI:
https://doi.org//10.32628/IJSRSET196617Keywords:
Tolaki Chicken, TGF-?2 Gene, Body Weight, Body Size MeasurementsAbstract
This study aims to identify the transforming growth factor-beta2 (TGF-β2) gene then associate it with body weight and body size measurements in Tolaki chicken. Tolaki chicken used in this study amounted to 60 hens, the results of hatching in the Lab. Halu Oleo University Poultry Unit, Kendari. Maintenance from 6 weeks to 12 weeks. Observed data include; body weight, back length, chest circumference, shank length, tibia length, femur length, and wing length, were measured every week during the study. The diversity of the TGF-β2 gene was identified using the PCR-RFLP method. The genotype frequency values ??of TT (39), TC (18), and CC (3), respectively 0.65, 0.30, and 0.05. The value of frequency of T allele and C allele are 0.80 and 0.20 of the total population, respectively. The results of the chi-square TGF-β2 gene are in Hardy-Weinberg balance. Statistical results showed no significant difference (P> 0.05) between the TT, TC and CC genotypes in the TGF-β2 gene with the weight parameters and body measurements of the Tolaki chicken.
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