Prevalence of Occult Hepatitis B Virus Infections Among Kidney Transplant Patients in Khartoum State, Sudan

Authors

  • Mashaer M Mustafa  Department of Medical microbiology, Faculty of Medical Laboratory Science, El Neelain University, Khartoum, Sudan
  • Abdel Rahim M El Hussein  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan
  • Isam M Elkhidir  Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Khalid A Enan  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan

DOI:

https://doi.org//10.32628/IJSRSET207112

Keywords:

HBV, ELISA, PCR, Renal transplant, Sudan.

Abstract

This study was carried out to detect occult hepatitis B virus (OHB) among renal transplant patients in Khartoum State, Sudan. Antigen capture enzyme linked immunosorbent assay (ELISA) competitive ELISA and polymerase chain reaction (PCR) were used to respectively detect hepatitis B surface antigen (HBsAg), ; Hepatitis B core antibody (HBcAb) and hepatitis B virus (HBV) DNA in 100 plasma samples collected from patients during the period from May to October 2018 . Out of the 100 patients sampled, 70 were males and 30 were females (age 15 to 65 years) and none of these patients showed signs of clinical hepatitis. The results showed that 3 out of the 100 samples were positive for HBsAg, and were subsequently excluded from the study. Out of the remaining HBsAg negative 97 samples, 39(40.2%) showed positive HBcAb and none (0%) tested positive to HBV DNA using competitive ELISA and PCR, respectively. These results indicated that more investigations including more patients from other transplant centers are needed to fully elucidate the situation of occult hepatitis B in renal transplant patients in Sudan.

References

  1. Dr Richard Hunt (2007). Hepatitis viruses. University of Southern California, Department of Pathology and Micro Biology, 2213.Availableat:http://pathmicro.med.sc.edu/virol/hepatitis-virus.htm.
  2. Mast EE, Alter MJ .Prevalence of hepatitis B virus among health Care personnel. In Ellis RW, ed. Hepatitis B vaccine in clinical practice. New York, Marcel Dekker.199;. pp. 295-307.
  3. Ke?Qin Hu, Occult hepatitis B virus infection and its clinical implications. J. Viral Hepatitis.2002, 9:243-257.
  4. Allain JP, Cox L. Challenges in hepatitis B detection among blood donors. Curr Opin Hematol. 2011;18:461-6
  5. Gonzalez R, Torres P, Castro E, Barbolla L,. et al. Efficacy of hepatitis B virus (HBV) DNA screening and characterization of acute and occult HBV infections among blood donors from Madrid, Spain Transfusion.2010;50: 221-30.
  6. Gerlich W, Wagner F, Chudy M, Harritshoj L, et al HBsAg non-reactive HBV infection in blooddonors: transmission and pathogenicity. J Med Virol. 2006;79: 32-6.
  7. Di Stefano M, Volpe A, Stallone G, Tartaglia L, et al, . Occult HBV infection in hemodialysis setting is marked by presence of isolated antibodies to HBcAg and HCV. J Nephrol.2009; 22: 381-386.
  8. Cibele Franz, Renata de Mello Perez, Mariano Gustavo Zalis, et al, of occult hepatitis B virus infection in kidney transplant recipients. Mem. Inst. Oswaldo Cruz [online]. 2013, 108:657-660. http://dx.doi.org/10.1590/0074-0276108052013019
  9. EunsinBae, Chang-Hun Park, Chang-Seok Ki, Sung-Joo Kim, et al(2012) Prevalence and clinical significance of occult hepatitis B virus infection among renal transplant recipients in Korea, Scandinavian Journal of Infectious Diseases.2012; 44: 788-792, DOI: 10.3109/00365548.2012.680488.
  10. Kazemi-Shirazi L, Petermann D, Müller CA . Hepatitis B virus DNA in sera and liver tissue of HBsAg negative patients with chronic hepatitis C. J Hepatol. 2000; 33: 785-790.
  11. Grob P, Jilg WH.. BornhakG. Gerken BG, Gerlich W et al Serological pattern anti HBc alone: report on workshop. J Med Virol 2000; 62: 437-455.
  12. Hassanein EH. Molecular Detection of Occult Hepatitis B Virus DNA in Haemodialysis Patients in El Gazeera State, Sudan. MSc Thesis. Sudan University of Science Technology, Khartoum,2013.
  13. Nafisa AI, Wafa IE . Occult Hepatitis B Virus infection in Sudanese haemodialysis patients. Lab. Med. J.2003; 1:43-49.
  14. Mohammed AA, Enan KA, Khair OA . Hussien MO, El Hussein AM2 and Elkhidir IM. Prevalence of occult hepatitis B virus (HBV) infections in haemodialysis patients in Khartoum State, Sudan from 2012 to 2015. 2015 6: 22-26,
  15. Ali R N, Rahmat Allah T, Osman T E, Almugadam B S. Serological detection of hepatitis B virus infection in patients under hemodialysis MOJ Biol Med. 2018;3:186?188.
  16. Sahr Hagmohamed SA*, Isam ME, M El Hussein AR and Khalid AE. Prevelance of occult Hepatitis B Virus (HBV) Infection among Hemodialysis Patients in Northern State, Sudan. Virol I mmunol J,2019,3(2): 000212.
  17. Ismail H,Soliman M, Ismail N . Occult hepatitis B virus infection in Egyptian hemodialysis patients with or without hepatitis C virus infection. Pathol. Lab. Med. Int.2010; 2:113-120.
  18. Ana Cecilia C, Maria RC, Marcílio FL, Regina CM et al. “Occult hepatitis B virus infection in hemodialysis patients in Recife, State of Pernambuco, Brazil”. Revista da Socieda de Brasileira de MedicinaTropical,2012; 45): 558-562.
  19. Cacciola I, Pollicino T, Squadrito G, Cerenzia G,., et al,. Occult hepatitis B virus infection in patientswith chronic hepatitis C liver disease N Engl J Med. 1999; 341: 22-26.
  20. Duhart Jr. BT, Honaker MR, Shokouh-Amiri MH, Riely CA, et al Retrospective evaluation of the risk of hepatitis B virus reactivation after transplantation.Transpl Infect Dis. 2003;5:1-6.
  21. Sengar DP, Couture RA, Lazarovits AI, Jindal SL Long-term patient and renal allograft survival in HBsAg infection: a recent update.Transplant Proc. 1989; 21: 3358-3359.

International Journal of Scientific Research in Science, Engineering and Technology

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Published

2020-01-30

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Volume 7, Issue 1, January-February-2020

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Research Articles

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[1]
Mashaer M Mustafa, Abdel Rahim M El Hussein, Isam M Elkhidir, Khalid A Enan, " Prevalence of Occult Hepatitis B Virus Infections Among Kidney Transplant Patients in Khartoum State, Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 7, Issue 1, pp.80-84, January-February-2020. Available at doi : https://doi.org/10.32628/IJSRSET207112