Association of TGF-β2 Gene Polymorphism with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens

Authors

  • Muh Akramullah  Master's Student of Animal Production and Technology Study Program, Postgraduate School, Bogor Agricultural University, Bogor, Indonesia.
  • Cece Sumantri  Teaching Staff of the Department of Animal Husbandry Production and Technology, Bogor Agricultural University, Bogor, Indonesia.
  • Niken Ulupi  Teaching Staff of the Faculty of Animal Husbandry, Halu Oleo University, Kendari, Indonesia.
  • Muh Amrullah Pagala  Teaching Staff of the Faculty of Animal Husbandry, Halu Oleo University, Kendari, Indonesia.

DOI:

https://doi.org//10.32628/IJSRSET20716

Keywords:

Tolaki chicken, TGF-β2 gene, SNP (640) T>C, Salmonella pullorum

Abstract

Transformation growth factor-beta 2 gene (TGF-β2) is a gene which belongs to the cytokine gene group. The present study aimed to identify Single Nucleotide Polymorphism (SNP) g. 640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) and associate its genotypes with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens. This study used blood samples collected from 70 chickens. Discussion was conducted on leucocyte concentration, leucocyte differentiation (heterophils, monocytes, lymphocytes, eosinophils, basophils and H/L ratio) and clearance test. Identification of Single Nucleotide Polymorphism of Transformation growth factor-beta 2 gene (TGF-β2) was performed by PCR-RFLP method. Association of (SNP) g. 640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) with leukocyte profiles and their differentiation and resistance to bacterial infections was carried out using analysis of variance with GLM (General Linear Model). SNP g.640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) was polymorphic and was in Hardy-Weinberg equilibrium. Based on molecular and biological testing, Tolaki chickens in genotypes (TT, TC and CC) have normal leucocyte concentration and differenciation and resistant to Salmonella pullorum.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2020-01-30

Issue

Volume 7, Issue 1, January-February-2020

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Research Articles

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How to Cite

[1]
Muh Akramullah, Cece Sumantri, Niken Ulupi, Muh Amrullah Pagala, " Association of TGF-β2 Gene Polymorphism with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 7, Issue 1, pp.46-54, January-February-2020. Available at doi : https://doi.org/10.32628/IJSRSET20716