Determination of Colour and Total Viable Counts in Fresh and Charcoal Flue Gases Treated Beef Using Digital Colour Meter

Authors

  • Catherine W. Njeru  Chemistry Department, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya
  • Isaac W. Mwangi  Chemistry Department, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya
  • Ruth W. Wanjau  Chemistry Department, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya
  • Grace N. Kiriro  Chemistry Department, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya
  • Gerald W. Mbugua  Chemistry Department, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya

DOI:

https://doi.org//10.32628/IJSRSET207470

Keywords:

Vitamins, Briquettes, Flue gases, Carboxy-myoglobin and Colour

Abstract

Flesh from animals is one of the nutritious foods rich in vitamin B complex, iron, zinc, selenium, phosphorous, fats and magnesium. Due to this high food value, it attracts bacteria, yeasts, molds and other lower life forms that infest on it and hence undergoes natural decay process fast. Poor handling practices by small scale enterprise in the beef industry contribute to fifty percent loss, excluding post slaughter losses by livestock farmers. Therefore, there is need to develop accessible preservation methods that are supported by available infrastructure in order to curb beef losses. This study used charcoal flue gases sourced from combustion of charcoal briquettes in prolonging beef shelf life. Fresh beef samples were purchased from local abattoirs and exposed to flue gases. The optimum treatment time was between 10 to 15 hours depending on beef load mass. The reaction of the carbon monoxide and carbon dioxide with beef functional groups was studied using color determination. The colour lightness (L*) and redness (a*) values were L* 44 and a* 9 values for fresh beef, after treatment L* 30 and a* 4.5 indicating a strong bond of carboxy-myoglobin and on day 35 the L* 23 and a* 1.5 indicated onset of beef spoilage. The mean total aerobic counts of fresh beef, after treatment and onset spoilage were significantly different (p<0.05) 4.16±4.20, 2.16±2.19 and 5.16±5.20 log CFU/cm2 respectively. The results from this study, was not more than 6.00 log CFU/cm2 approved safe microbial standards of beef for consumption by FAO.

References

  1. Adu-Gyamfi, A., Torgby-Tetteh, W. and Appiah, V. (2012). Microbiological quality of chicken sold in Accra and determination of D10-value of E. coli. Food and Nutrition Sciences, 3(05), 693-698
  2. Ercolini, D., Russo, F., Torrieri, E., Masi, P. and Villani, F. (2006). Changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Applied and Environmental Microbiology, 72(7), 4663-4671
  3. FAO (2007). Meat processing technology for small–to-medium-scale producers. [http://www.fao.org/docrep/010/ai407e/ai407e00.htm] site visited 14/03/2020.
  4. Giannuzzi, L., Pinotti, A. and Zaritzky, N. (1998). Mathematical modelling of microbial growth in packaged refrigerated beef stored at different temperatures. International Journal of Food Microbiology, 39(1-2), 101-110.
  5. Hernández-Macedo, M. L., Barancelli, G. V. and Contreras-Castillo, C. J. (2011). Microbial deterioration of vacuum-packaged chilled beef cuts and techniques for microbiota detection and characterization: a review. Brazilian Journal of Microbiology, 42(1), 1-11
  6. IS 15478-2 (2004): Meat and Meat Products - Sampling and Preparation of Test Samples, Part 2: Preparation of Test Samples for Microbiological Examination
  7. ISO 3100-1:1991. Meat and meat products - Sampling and preparation of test samples
  8. Jayasingh, P.; Cornforth, D.P.; Carpenter, C.E. and Whittier, D. (2001). Evaluation of carbon monoxide treatment in modified atmosphere packaging or vacuum packaging to increase color stability of fresh beef. Meat Science, in press, 59(3), 317-324.
  9. Jones, R. J. (2004). Observations on the succession dynamics of lactic acid bacteria populations in chill-stored vacuum-packaged beef. International Journal of Food Microbiology, 90(3), 273-282.
  10. King Jr, A. D. and Nagel, C. W. (1967). Growth inhibition of a Pseudomonas by carbon dioxide. Journal of Food Science, 32(5), 575-579
  11. Lean, Michael E.J. (2019). Principles of Human Nutrition. Medicine, 47(3), 140-144.
  12. Mancini, R. A. and Hunt, M. (2005). Current research in meat color. Meat Science, 71(1), 100-121.
  13. Martínez, L., Djenane, D., Cilla, I., Beltrán, J. A. and Roncalés, P. (2005). Effect of different concentrations of carbon dioxide and low concentration of carbon monoxide on the shelf-life of fresh pork sausages packaged in modified atmosphere. Meat Science, 71(3), 563-570.
  14. Parry, R. T. (Ed.). (2012). Principles and applications of modified atmosphere packaging of foods. 2nd ed (pp. 1-305) Springer Science and Business Media.
  15. Pipek, P., Staruch, L. and Izumimoto, M. (2008). Stabilization of minced meat colour by carbon monoxide.Czech. Journal of Food Science, 26(5), 333-338.
  16. Sarker, M. S., Fazl-I-Rabbi, S. and Siddika (2016). A. Chemical and microbial analysis of dry meat. Journal of Natural and Social Sciences, 3(3), 86-89.
  17. Sørheim, O., Nissen, H. and Nesbakken, T. (1999). The storage life of beef and pork packaged in an atmosphere with low carbon monoxide and high carbon dioxide. Meat Science, 52(2), 157-164.

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Published

2020-08-30

Issue

Section

Research Articles

How to Cite

[1]
Catherine W. Njeru, Isaac W. Mwangi, Ruth W. Wanjau, Grace N. Kiriro, Gerald W. Mbugua, " Determination of Colour and Total Viable Counts in Fresh and Charcoal Flue Gases Treated Beef Using Digital Colour Meter, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 7, Issue 4, pp.274-278, July-August-2020. Available at doi : https://doi.org/10.32628/IJSRSET207470