Effect of Chemical Mutagens on Seedling Height in Cluster Beans { Cyamopsis Tetragonoloba (L) Taub.} by Mutation Breeding
Keywords:
ethyl methanesulphonate, Golden Early – 36, Harit Rani, cluster bean.Abstract
In the present study two varieties of cluster bean, namely, Golden Early – 36 (GE-36) and Harit Rani (HR) were used to induce genetic variability. For present study two chemical mutagens, namely, ethyl methanesulphonate (EMS) of different concentrations such as 0.05%, 0.10% and 0.15% and sodium azide (SA) of concentrations such as 0.01%, 0.02% and 0.03% were tried. Seeds from each treatment were sown in field following randomized block design (RBD) with three replications along with control as the M1 generation. Further two consequent generations were taken, M2 and M3 generations respectively. In M1generation seedling height was studied. After 10 days of duration, height of seedlings was noted. The seedling height decreased as the concentration(%) of the two mutagens increased in majority of the treatments in both the varieties of cluster bean.
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