Molecular Detection of Torque Teno Virus (TTV) in Plasma of People in Contact with Domestic Village Chickens using Polymerase Chain Reaction (PCR)

Authors

  • Ahmed Y Mohamed  Department of Microbiology, Faculty of Medical Laboratory Sciences El Neelalin University, Sudan
  • Abdel Rahim M. El Hussein  Department of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan
  • Isam M Elkhidir  Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Khalid A Enan  

Keywords:

West Nile Viruses (WNV), IgM .ELISA and Real-Time PCR.

Abstract

Background : Torque teno virus (TTV) was first detected in 1997 in Japan in a patient with post-transfusion hepatitis. The virus was also detected in the liver and blood of people with hepatic pathologies of unknown etiology. In addition to man,TTV was also found to infect domestic and wild animals including cattle, chickens, pigs, cats, and dogs, etc.
Objective : This study was designed to detect TTV DNA among people who have contact with domestic chickens in Khartoum State during the period December 2016toJune 2017.
Method : A total 100 blood samples were collected,out of these 60 blood samples were collected from people who had contact with domestic village chickens and 40 blood samples were collected from people who had no contact and did not eat chickens. The samples were tested for TTV DNA using nested Polymerase Chain Reaction (nested- PCR) .
Result : TTV DNA was detected in 3(5 %) out of 60 blood samples from people who have contact with domestic chickens and all the samples were negative in the group of people who had no contact and  did not eat chickens.
Conclusion : We observed low TTV prevalence and no association between contact with domestic chickens and TTV infection prevalence was  detected.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-12-31

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Volume 3, Issue 8, November-December-2017

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Research Articles

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How to Cite

[1]
Ahmed Y Mohamed, Abdel Rahim M. El Hussein, Isam M Elkhidir, Khalid A Enan, " Molecular Detection of Torque Teno Virus (TTV) in Plasma of People in Contact with Domestic Village Chickens using Polymerase Chain Reaction (PCR), International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.250-254, November-December-2017.