Invitro Anti-arthritic and Anti-inflammatory activities of Rhynchosia rufescens (Willd.)DC. Seed extract on Inhibition of Protein Denaturation

Authors

  • Panneerselvam M  Department of Botany, Govt. Arts College, Tiruvannamalai, Tamil Nadu, India
  • Shanthi K  Department of Botany, Govt. Arts College, Tiruvannamalai, Tamil Nadu, India

Keywords:

Anti-arthritic, Anti-inflammatory, Protein denaturation, Diclofenac sodium and Rhynchosia rufescens.

Abstract

The Rheumatic arthritis is an auto immune inflammatory disorder which cause destruction of cartilage and bone damage with risk of infections. Inflammation is the response of living tissues to injury and is a complex pathophysiological process associated with extravasation of fluid and proteins and accumulation of leukocytes at the site of injury. In the present study, the invitro anti-arthritic and anti-inflammatory activity of the ethanolic seed extract of Rhynchosia rufescens was evaluated with the inhibition of protein denaturation. Diclofenac sodium was used as control drug. The methanolic seed extract shows high percentage of inhibition of protein denaturation in the concentration of 100µl/ml and significant result of anti-inflammatory activity at 250µl/ml concentration. The results suggested that the Rhynchosia rufescens would be a valuable herbal plant for scientific investigations.

References

  1. Agarwal, A., (2005):Pharma Times 37(6): 9-11.
  2. Chopra, R.N., Nayar, S.L. and Chopra, I.C. (1956): In Glossary of Indian medicinal plants, Council of Scientific and Industrial Research, New Delhi.1;197
  3. Murugan P, Rajesha A, Athiperumalsami T, and Mohan VR: Screening of Certain Ethnomedicinal plants for antibacterial activity. Ethnobotanical Leaflets, 2008; 12: 433-438.
  4. 3. Firestein GS: Evolving concepts of rheumatoid arthritis. Nature, 2003; 356-361.
  5. 4. Hard E: Extra articular manifestations of rheumatoid arthritis. Arthritis Rheum. 1979; 8: 151-176.
  6. [18] Ashley NT, Weil ZM, Nelson RJ. Inflammation: mechanisms, costs, and natural variation. Annu Rev Ecol Evol Syst 2012; 43: 385-406.
  7. [19] Williams LA, O’Connar A, Latore L, Dennis O, Ringer S, Whittaker JA, et al. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med J 2008; 57: 327-31.
  8. Kalidass, C. and Mohan, V. R.2012. Biochemical composition and nutritional assessment of selected under-utilized food legume of the genus Rhynchosia, International Food Research Journal. 19 (3): 977-984
  9. Mizushima, Y., Kobayashi, M. 1968. Interaction of anti inflammatory drugs with serum proteins, especially with some biologically active proteins. J. Pharma. Pharmacol., 20: 169 173.
  10. Padmanabhan P, Jangle SN. Evaluation of in-vitro anti-inflammatory activity of herbal preparation, a combination of four herbal plants. Int J Basic Appl Med Sci 2012; 2(1): 109-16.
  11. Vivacons M, Moreno J. J Free, Rad Biol Med 2005; 39: 91-97.

International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-10-31

Issue

Volume 3, Issue 6, September-October-2017

Section

Research Articles

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How to Cite

[1]
Panneerselvam M, Shanthi K, " Invitro Anti-arthritic and Anti-inflammatory activities of Rhynchosia rufescens (Willd.)DC. Seed extract on Inhibition of Protein Denaturation, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 6, pp.409-412, September-October-2017.