Oxidative Stress Response Induced by PEG in Arabidopsis Engineered with the Cu/Zn-SOD and Mn-SOD Antioxidant System Genes

Authors

  • Amrina Shafi  Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India
  • Mudasir A Mir  Centre for Plant Biotechnology, Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, India
  • Insha Zahoor  Department of Biotechnology, School of Biological Sciences, University of Kashmir, Srinagar, Jammu and Kashmir, 190006, India

Keywords:

Cu/Zn-SOD, Mn-SOD, Water stress, PEG, ROS, Germination

Abstract

Among abiotic stresses, water stress is one of the major stress causing growth retardation and yield loss of plants along with an increased accumulation of reactive oxygen species (ROS). To cope with oxidative stress, plants employ a host of antioxidants and enzymatic systems. This consists of enzymes like superoxide dismutases, catalases, peroxidases. In the present study, the genetically engineered Arabidopsis with copper-zinc superoxide dismutase (Cu/Zn-SOD) and manganese superoxide dismutase (Mn-SOD) genes isolated from Potentilla atrosanguinea and Camellia sinensis respectively, both of which are high altitude cold niche area plants of Western Himalaya, were evaluated against drought stress induced by polyethylene glycol (PEG). The genes, Cu/Zn-SOD and Mn-SOD were. It was found that the transgenic plants over-expressing both the genes were more tolerant to PEG-induced stress during growth and development. In both transgenic plants higher levels of total antioxidant enzyme activities, chlorophyll content, total soluble sugars, proline content and lower levels of ROS, ion leakage were recorded when compared to the WT during PEG stress. Transgenic plants showed 2-3 fold increase in SOD activity resulting in the enhanced stress tolerance to PEG stress than WT. In terms of growth and development in number of leaves, rosette area and root length, which was observed to be significantly higher in transgenic lines compared to WT. Also, the transgenic lines showed higher germination percentage at various levels of PEG. Overall, Cu/Zn-SOD and Mn-SODtransgenic lines were able to express greater drought tolerance and thus the present work would pave way for the judicious use of these genes effectively into the relevant crop plants leading to optimum growth and enhanced yield under environmentally stressed conditions.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2017-12-31

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Volume 3, Issue 8, November-December-2017

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Research Articles

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[1]
Amrina Shafi, Mudasir A Mir, Insha Zahoor, " Oxidative Stress Response Induced by PEG in Arabidopsis Engineered with the Cu/Zn-SOD and Mn-SOD Antioxidant System Genes, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 3, Issue 8, pp.40-46, November-December-2017.