Preliminary Phytochemical Evaluation and HPTLC Fingerprint Profile of Jasminum azoricum L

Authors

  • N Hari  Department of Botany, CMS College Kottayam, Kerala, India
  • Vandana P Nair  Department of Botany, CMS College Kottayam, Kerala, India

Keywords:

Jasminum azoricum , HPTLC, phytoconstituents, derivatization

Abstract

The present study was aimed to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of methanol extracts of leaves of Jasminum azoricum. This study was planned to develop a HPTLC fingerprint profile of extracts in the best solvent system in Toluene: Ethyl acetate: Formic acid: Methanol:: 7:5:1:0.5. The HPTLC method for the separation of the active constituents in extract has been developed and TLC of these extracts on silica gel pre-coated aluminium plates of MERCK by automatic TLC applicator and using solvent system HPTLC profiling of the extract confirm about the presence of various phytochemicals. HPTLC fingerprint scanned at 254 nm wavelength for methanol (leaf) and revealed the existence of six phytoconstituents whose Rf  values ranged from 0.44 to 0.81 and in 366 nm it revealed the existence of eight constituents whose Rf values ranged from 0.44 to 0.87. Further  research is necessary to extract the compounds from J. azoricum and it will give programmed for advanced  phytochemical and pharmaceutical applications.

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-02-28

Issue

Volume 4, Issue 1, January-February-2018

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
N Hari, Vandana P Nair, " Preliminary Phytochemical Evaluation and HPTLC Fingerprint Profile of Jasminum azoricum L, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 1, pp.791-795, January-February-2018.