Molecular Detection of NontuberculousMycobacteria (Mycobacterium Chelonae, MycobacteriumAbscessus Group and Mycobacterium Fortuitum Complex) Among Patient with Post-Operative Wound Infection in Khartoum State - Sudan

Authors

  • Mohamed Mostafa  Department of Medical microbiology, Faculty of Medical Laboratory Science, El Nellin University, Khartoum, Sudan
  • Abdel Rahim M El Hussein  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
  • Mohamed O. Mustafa  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan
  • Isam M Elkhidir  Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Khalid A Enan  Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, Khartoum, Sudan

Keywords:

NTM, CTAB, DNA, RGM, MFC, MC–MAG, PCR

Abstract

Background
Nearly 100 mycobacterium belong to rapidly growing mycobacterium (RGM), the most common pathogens being M. chelonae M. abscessus groups and M. fortitum complex, M. abscessus is more resistant to antibiotic and disinfection. Nontuberculosis mycobacteria have been isolated from various sites viz., cutaneous and soft tissue infection, after skin injury following inoculation and after surgical procedures and also, known to cause systemic infection in AIDS and immunocompromised patients. Surgical site infection due to atypical mycobacterium usually has a long incubation period.
Material and Method
Swaps from infected wound surgical were collected randomly from 40 patients, the bacterial DNA were extracted from the samples by using the CTAB method. The different primer designed to detect M. abscessus, M. chelonae, and M. fortitum complex. Were used DNA amplification was performed under different optimization PCR condition for different nontuberculosis mycobacteria species. The PCR products were separated by electrophoresis using 2% agarose and the products were visualized in gel documentation system. A 100pb ladder was used for comparison of product length.
Result
The prevalence of NTM infection among patients with post-operative wounds was 17.5% for M. abscessus, 15% for M. fortitum and 0% for M. chelonae. Infection was higher in males (17.5%) in comparison to females (7.5%). NTM organism were detected in all delayed healing periods from 7 days to two years.

Conclusion

Our result shows that the M. abscessus and M. fortitum infection were found among patient with post operative wounds in Sudan. The infection are higher in males than females. Further investigation is needed to identify strains of NTM found in hospitals for proper control it and to study whether this microorganism can cause (nosocomial infection).

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2018-04-30

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Volume 4, Issue 4, March-April-2018

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Research Articles

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[1]
Mohamed Mostafa, Abdel Rahim M El Hussein, Mohamed O. Mustafa, Isam M Elkhidir, Khalid A Enan, " Molecular Detection of NontuberculousMycobacteria (Mycobacterium Chelonae, MycobacteriumAbscessus Group and Mycobacterium Fortuitum Complex) Among Patient with Post-Operative Wound Infection in Khartoum State - Sudan, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 4, Issue 4, pp.183-189, March-April-2018.