Studies on effect of Copper Oxide nanoparticles on Chickpea Cicer arietinum seed germination and root elongation
Keywords:
Copper Oxide Nanoparticles, Cicer arietinum , Seed germination .Abstract
Nanotechnology is an emerging branch of science. Nanoparticles have unique physical and chemical properties and thus used in diverse applications. In present study copper oxide nanoparticles were used to determine their effect on seed germination and root elongation. 5ppm and 10ppm concentrations of CuO NP prepared in distilled water. 20 seeds were treated with 5ppm and 10ppm concentration respectively for 30 min. 10 treated seeds were transferred aseptically per sterilized petriplates containing moist filter paper and labeled respectively. Similar experiment was carried out using distilled water as control. The experiment was carried out in duplicate. The plates were kept in dark for 7 days. 5ppm, 10ppm as well as distilled water treated seeds showed 100 % germination. The highest mean root length 16.93 cm was observed in 5ppm CuO NP treated seeds followed by 13.69 cm in 10ppm CuO NP treated seeds. While in control the root length was found to be 11.93cm. The current experiment reveled that these concentrations of CuO NP does not affect the germination but enhance the root elongation mechanism.
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