Characteristics of Ini Vitro Fermentative Digestibility of Odot Grass (Pennisetum Purpureum Cv. Mott) At Different Planting Spacing and Defoliation Age

Authors

  • La Malesi  Animal Science Department, Faculty of Animal Science, Halu Oleo University, Kendari, Sulawesi Tenggara
  • Takdir Saili  Animal Science Department, Faculty of Animal Science, Halu Oleo University, Kendari, Sulawesi Tenggara
  • Ali Bain  Animal Science Department, Faculty of Animal Science, Halu Oleo University, Kendari, Sulawesi Tenggara
  • Tresjia C Rakian  Soil Science Department, Fakulty of Agriculture, Halu Oleo University, Kendari,Sulawesi Tenggara

DOI:

https://doi.org//10.32628/IJSRSET207225

Keywords:

Digestibility, fermentative, in vitro, nutrient, pennisetum purpureum cv. Mott

Abstract

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (p<0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (p>0.05), whereas among the defoliation age treatments were significantly different (p<0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.

References

  1. Akinfemi, A., A.O. Adesanya and V.E. Aya, 2009. Use of an in vitro gas production technique to evaluate some Nigerian feedstuffs. Am.-Eurasian J. Sci. Res., 4(4): 240-245. Retrieved May 6, 2019, from https://www.researchgate.net/publication/239530733
  2. Bain, A. Wiryawan, K.G. Astuti, D.A, Arman, C dan Suharti, S. 2017. Fermentability and Nutrient Digestibility of Ration Supplemented with Soybean Oil Calcium Soap and Cashew Fruit Flour. Pak. J. Nutr., 16(12): 945-953, 2017. Retrieved August 15, 2019 https://scialert.net/fulltext/?doi=pjn.2017.945.953
  3. Bain, A. Wiryawan, K.G. Astuti, D.A, Arman, C dan Suharti, S. 2018. Optimization of the use of soybean calcium soap level in ration on fermentation characteristics, microbial population, and nutrient digestibility in vitro by using a ruminal fluid of Bali cattle (in Indonesian).
  4. Bilal, M.Q. 2009.Effect of molasses and corn as silage additives on the characteristics of Mott dwarf elephant grass silage at different fermentation periods. Vet. J. 29: 19 - 23. Retrieved August 15, 2019. https://core.ac.uk/download/pdf/25877004.pdf
  5. Budiman, R.D. Sutrisno, S.P.S. Budhi, A. Indrianto. 2012. Morphological characteristics, productivity and quality of three Napier grass (Pennisetum purpureum Schum) cultivars harvested at a different age. J. Indonesian Trop. Anim. Agric. 37:294-301. www.jppt.undip.ac.id/pdf/37(4)2012p294-301.pdf
  6. Conway, E.J. 1962. Microdiffusion Analysis and Volumetric Error.3rd Ed. Crostay Loskwood and Sows.Ltd. London.
  7. Djajanegara, A., M. Rangkuti., Siregar, Soedarsono, S.K. Sejati. 1998. Feed and its Factors (in Indonesia). Ruminant Scientific Meeting. Department of Agriculture, Bogor.
  8. Firsoni dan R. Yunita. 2014. Degradability test of complete feed containing Chromolaena odorata leaves in vitro (in Indonesia). Jurnal Peternakan Indonesia, Juni 2014, ISSN 1907-1760.16(2).89-93.
  9. Firsoni. 2014. Effect of Chromolaena Odorata application in the concentrate on methane gas production in vitro (in Indonesia). Proceedings of National Seminar of Feed Sinergy and Renewable Energy. P ; 424-427.
  10. Firsoni, L. Puspitasari dan L. Andini. 2011. Effect of paitan leaves (Tithoniadiversifolia (HEMSLEY) A. GRAY) and Kelor (Moringa oleifera, LAMK) in complete feed in vitro (in Indonesia). Proceedings of National Seminar of Livestock and Veterinary Technology. ISBN 978-602-8475-46-4.
  11. Getachew, G., P. H. Robinson, E. J. DePetersand S. J. Taylor. 2004. Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds, Animal Feed Science and Technology, 111 (2004) 57-71. https://doi.org/10.1016/S0377-8401(03)00217-7
  12. Haaland GL, Tyrrel HF, Moe PW, Wheeler WE. 1982. Effect of crude protein level and limestone buffer in diets feed at two levels intake on rumen pH, ammonia-nitrogen, buffering capacity and VFA concentration of cattle. J Anim Sci. 55(4): 943.
  13. Hariadi, B. Tj. & B. Santoso. 2010. Evaluation of tropical plants containing tannin on in vitro methanogenesis and fermen¬tation parameters using rumen fluid. J. Sci. Food. Agric. 90:456-461.
  14. Halim, M.R.A, Samsuri S, Bakar I.A. 2013. Yield and nutritive quality of nine Napier grass varieties in Malaysia. J. Anim Sci. Malaysian 16(2) : 37-44. http://msap.my/mjas16-2/5%28YIELD-HalimRidzwan_37-44%29-rev3.pdf
  15. Hartadi, H., Reksohadiprodjo, S. Lebdosoekoyo, Tillman A. D, Kearl L.C, Harris L. E. 1980. Tables of Animal Feed Stuff for Indonesia (in Indonesia). Logan Utah. Utah University.
  16. Hindratiningrum, N., M. Bata dan S. A. Santosa. 2011. Rumen fermentation product and microbial protein production of local cattle fed with ammoniated rice straw and some feedstuff of energy source (in Indonesia). Agripet 11(2) : 29-34
  17. Kozloski, G.V., J. Perettoni, L.M.B. Sanchez. (2006). Influence of regrowth age on nutritive value of dwarf elephant grass (Pennisetum purpureum schum cv. Mott) consumed by lamb. Journal of Animal Feed Science, 119 : 1-11. https://doi.org/10.1016/j.anifeedsci.2004.12.012
  18. McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, L.A. Sinclair and R.G. Wilkinson 2010. Animal Nutrition.7th Ed. Gosport (UK): Ashford Color Pr. Pearson Education Ltd., England.
  19. Menke, K. H., Raab, L. L., Salewski., A., Steingas, H., and Schneider, W., 2009. The estimation of digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor. J. Agric. Sci. 93:217-222. https://doi.org/10.1017/S0021859600086305
  20. Owens, F.N. dan A.L. Goetsch. 1988. Ruminal Fermentation. In D.C. Church Ed.The Ruminant Animal Digestive Physiology and Nutrition. A. Reston Book. Prentice-Hall, Eaglewood Cliffs, New Jersey.
  21. Parakkasi, A. 1999. Feed and Nutrition Science of Ruminant Animal (in Indonesian). UI Press, Jakarta.
  22. Pellikaana, W. F., W. H. Hendriksa, G.Uwimanaa, L. J. G. M. Bongersa, P. M.Beckerc, and J. W. Conea. 2011. A novel method to determine simultaneously methane production during in vitro gas production using fully automated equipment, Animal Feed Science and technology 168 ; 196- 205. https://doi.org/10.1016/j.anifeedsci.2011.04.096
  23. Rahmadi, D., Sunarso., J. Achmadi., A. Muktiani., M. Christiyanto dan Surono. 2004. Basic Ruminology (in Indonesian). Undip Press, Semarang.
  24. Sutardi, T. 1993. Utilization of horticultural by-product as ruminant feed. Proceedings of Exhibition of Livestock Production and Technology, page 102-121.
  25. Sirait J, Tarigan A, Simanihuruk K. 2015. Morphology characteristics of mini king grass (Pennisetum purpureum cv. Mott) at different planting spacing in two agroecosystems in North Sumatra (in Indonesia).
  26. Steel, R. G. D dan J. H. Torrie. 1995. Principal and Procedure of Statistics (in Indonesian). Translation of Sumantri B. PT. Gramedia Pustaka Utama, Jakarta.
  27. Sulistyawati, T.A. dan Mariyono. 2013. The productivity of superior grass in the mine area (in Indonesian). National Seminar of Livestock and Veterinary Technology, page 455-460
  28. Tilley, J. M. A dan R. A. Terry. 1963. A Two Stages Technique For The In Vitro Digestion of Forage Crops. J. of British Grassland 18: 104 – 111. https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2494.1963.tb00335.x
  29. Wanapat, M., S. Kang and S. Polyorach.2013. Development of feeding systems and strategies of supplementation to enhance rumen fermentation and ruminant production in the tropics, Journal of Animal Science and Biotechnology, 4:32. https://link.springer.com/article/10.1186/2049-1891-4-32
  30. Wahyuni, D. S. 2008. In vitro fermentability and degradability and production of microbial biomass of combination of complete feed and natural grass, rich-nutrient concentrate supplement (in Indonesia). IPB University, Bogor.

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Published

2020-04-30

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Research Articles

How to Cite

[1]
La Malesi, Takdir Saili, Ali Bain, Tresjia C Rakian, " Characteristics of Ini Vitro Fermentative Digestibility of Odot Grass (Pennisetum Purpureum Cv. Mott) At Different Planting Spacing and Defoliation Age, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 7, Issue 2, pp.368-376, March-April-2020. Available at doi : https://doi.org/10.32628/IJSRSET207225