Changes of Physiological Characteristic Indexes in the Aging Process of the Liquid Spawn of Auricularia auricula

Authors

  • Jong Chol  College of Agriculture, Haeju Kim JeWon University of Agriculture, Haeju 999093, Democratic People's Republic of Korea
  • Jin MyongIl  Sariwon Kye UngSang University of Agriculture, Sariwon 950003, Democratic People's Republic of Korea
  • Choe SunIl  Sariwon Kye UngSang University of Agriculture, Sariwon 950003, Democratic People's Republic of Korea
  • Zhu HaiFeng  College of Resources and Environment, Northeast Agricultural University, Harbin, China
  • Li DeTian  College of Resources and Environment, Northeast Agricultural University, Harbin, China
  • Jo TaeYun  College of Agriculture, Haeju Kim JeWon University of Agriculture, Haeju 999093, Democratic People's Republic of Korea
  • Kim GyongChol  College of Agriculture, Haeju Kim JeWon University of Agriculture, Haeju 999093, Democratic People's Republic of Korea

DOI:

https://doi.org/10.32628/IJSRSET218141

Keywords:

Auricularia auricula, liquid spawn, aging, physiological index, edible fungus

Abstract

In order to explore the change rule of physiological indexes during the aging process of the liquid spawn of Auricularia auricula, this test chooses three species of Auricularia auricula(Hei29, Heiweibanjin and Heiweidanpian), which treasured the activities of antioxidant enzymes, antioxidant substances, membrane peroxidation degree and reactive oxygen concentration during different mycelia age. The conclusion showed that: at the same mycelial age, the vitality of Heiweidanpian was the strongest, Hei29 followed, and Heiweibanjin was the weakest; with the increase of mycelia age, Catalase (CAT) activity, Ascorbic Acid(ASA) content, Malondialdehyde (MDA) content and O2- production rate of the three kinds of Auricularia auricula increased continuously. Among them, ASA content, MDA content and O2- production rate increased significantly, while Superoxide Dismutase (SOD) activity decreased significantly; although the Proline (Pro) content and electrical conductivity of the three kinds of Auricularia auricula showed different trends, which decreased significantly at 14 to 16d and increased significantly at 8 to 10d, respectively, and H2O2 content increased significantly at 12 to 16d. It can be seen that SOD activity, CAT activity, ASA content, MDA content and O2- production rate have significant regular changes during the aging process of the liquid spawn of Auricularia auricula.

The quantitative analysis found that when the MDA content is 1.42~1.72 μmol·g-1, the H2O2 content is 3.4~4.3 μmol·g-1 or the O2- production rate is 9.5~12.1 μmol·g-1·min-1, it can be considered Auricularia auricula just started to age or the aging degree is relatively low.

References

  1. Fang Jie Yao, Li-Xin Lu, et al. Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae, Mycobiology. 2018, 46(1): 72-78.
  2. Andrea Hamann, et al. Apoptosis pathways in fungal growth, development and ageing. Trends in Microbiology, 2008, 16(6):276-283.
  3. Heinz D Osiewacz. Aging in fungi: role of mitochondria in Podospora anserine. Mechanisms of Ageing and Development, 2012, 123(7):755-764
  4. Ahmad P, Jaleel C A, Salem M A, et al. Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical Reviews in Biotechnology, 2010, 30(3): 161-175.
  5. Zhang Y. The Research of Reactive Oxygen Species (ROS) in plants. Current Biotechnology, 2011, 1(4): 242-248.
  6. Namal P, et al. Phagocytosis enhancement, endotoxin tolerance, and signal mechanisms of immunologically active glucuronoxylomannan from Auricularia auricula-judae, International Journal of Biological Macromolecules, 2020, 165:495-505
  7. Liu Zhanli, et al. Changes in color, antioxidant, and free radical scavenging enzyme activity of mushrooms under high oxygen modified atmospheres, Postharvest Biology and Technology. 2012, 69:1-6.
  8. Xu Jie, et al. Salinity relief aniline induced oxidative stress in Suaeda salsa: Activities of antioxidative enzyme and EPR measurements. Ecotoxicology and Environmental Safety, 2020, 205:35-45
  9. Hameed A?Iqbal N?Malik S A. Mannose-induced modulations in antioxidants?protease activity?lipid peroxidation?and total phenolics in etiolated wheat leaves. Journal of plant growth regulation?2009, 28(1): 58-65.
  10. Tai T H. Evaluation of seedling cold tolerance in rice cultivars: a comparison of visual ratings and quantitative indicators of physiological changes. Euphytica, 2011, 178(3): 437-447.
  11. Madosa Emilian, et al. Studies on drought tolerance assessed by testing proline content in pepper (Capsicum annuum L.). Current Opinion in Biotechnology, 2013, 24:56-63
  12. Mohammad Rezaee,et al. Development of an electrical conductivity screening test for mine waste assessments. Chemosphere, 2016, 160:13-21.
  13. Xia Fangshan, et al. Effects of non-enzymatic scavengers of reactive oxygen species on aging seeds, Pratacultural Science 2016, 33 (6), 1126-1135.
  14. Gila Idelman,et al. Bilirubin inhibits the up-regulation of inducible nitric oxide synthase by scavenging reactive oxygen species generated by the toll-like receptor 4-dependent activation of NADPH oxidase. Redox Biology, 2015, 5:398-408
  15. Yin Yahui, An Wentao, et al. Changes of antioxidant enzyme activities in different algebraic of Saccharomyces cerevisiae, Food Science and Technology, 2013, 38(8):38-47.
  16. Jiang M, et al. Antioxidant effects of proline accumulation in rice seedlings under oxidative stress, Journal of Plant Physiology, 1997, (4):347-352.
  17. Preeya Kaewnaree, et al. Electrolyte leakage and fatty acid changing associated with seed germination in accelerated aging sweet pepper seeds. Journal of Biotechnology, 2008, 136:108-121.
  18. Pasquini S, Mizzou M, et al. Seed storage in polyethylene bags of a recalcitrant species (Quercus ilex): analysis of some bio-energetic and oxidative parameters. Acta physiological Plantarum. 2012?34(5):1963-1974.
  19. Botella, M. A, Rosado. A, et al. Plant adaptive responses to salinity stress. Plant abiotic stress. 2005(1):37-70.

International Journal of Scientific Research in Science, Engineering and Technology

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Published

2021-02-28

Issue

Volume 8, Issue 1, January-February-2021

Section

Research Articles

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How to Cite

[1]
Jong Chol, Jin MyongIl, Choe SunIl, Zhu HaiFeng, Li DeTian, Jo TaeYun, Kim GyongChol "Changes of Physiological Characteristic Indexes in the Aging Process of the Liquid Spawn of Auricularia auricula " International Journal of Scientific Research in Science, Engineering and Technology (IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 8, Issue 1, pp.204-215, January-February-2021. Available at doi : https://doi.org/10.32628/IJSRSET218141